دورية أكاديمية
Pharmacological Investigation of CC-LAAO, an L-Amino Acid Oxidase from Cerastes cerastes Snake Venom
| العنوان: | Pharmacological Investigation of CC-LAAO, an L-Amino Acid Oxidase from Cerastes cerastes Snake Venom |
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| المؤلفون: | Abdelkafi-Koubaa, Zaineb, Elbini-Dhouib, Ines, Souid, Soumaya, Jebali, Jed, Doghri, Raoudha, Srairi-Abid, Najet, Essafi-Benkhadir, Khadija, Micheau, Olivier, Marrakchi, Naziha |
| المساهمون: | Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Université de Tunis El Manar (UTM), University of Louisiana, Salah Azaiez Institute, Lipides - Nutrition - Cancer Dijon - U1231 (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, The authors are grateful to the Ministry of Higher Education and Scientific Research of Tunisia for financial support (LR20IPT01). |
| المصدر: | EISSN: 2072-6651 ; Toxins ; https://inserm.hal.science/inserm-03516725Test ; Toxins, 2021, 13 (12), pp.904. ⟨10.3390/toxins13120904⟩ |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2021 |
| المجموعة: | Université de Bourgogne (UB): HAL |
| مصطلحات موضوعية: | SV-LAAOs, apoptosis, envenomation, glioblastoma cells, toxicity, MESH: Alanine Transaminase, MESH: Animals, MESH: L-Lactate Dehydrogenase, MESH: Male, MESH: Mice, MESH: Viper Venoms, MESH: Viperidae, MESH: Cell Line, Tumor, MESH: Cell Survival, MESH: Chick Embryo, MESH: Creatinine, MESH: Edema, MESH: Hemorrhage, MESH: Humans, MESH: L-Amino Acid Oxidase, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| الوصف: | International audience ; Snake venom proteins, which are responsible for deadly snakebite envenomation, induce severe injuries including neurotoxicity, myotoxicity, cardiotoxicity, hemorrhage, and the disruption of blood homeostasis. Yet, many snake-venom proteins have been developed as potential drugs for treating human diseases due to their pharmacological effects. In this study, we evaluated the use of, an L-amino acid oxidase isolated from Cerastes cerastes snake venom CC-LAAO, as a potential anti-glioblastoma drug, by investigating its in vivo and in vitro pharmacological effects. Our results showed that acute exposure to CC-LAAO at 1 and 2.5 µg/mL does not induce significant toxicity on vital organs, as indicated by the murine blood parameters including aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH) activities, and creatinine levels. The histopathological examination demonstrated that only at high concentrations did CC-LAAO induce inflammation and necrosis in several organs of the test subjects. Interestingly, when tested on human glioblastoma U87 cells, CC-LAAO induced a dose-dependent apoptotic effect through the H2O2 generated during the enzymatic reaction. Taken altogether, our data indicated that low concentration of CC-LAAO may be safe and may have potential in the development of anti-glioblastoma agents. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34941741; inserm-03516725; https://inserm.hal.science/inserm-03516725Test; https://inserm.hal.science/inserm-03516725/documentTest; https://inserm.hal.science/inserm-03516725/file/toxins-13-00904.pdfTest; PUBMED: 34941741; PUBMEDCENTRAL: PMC8704781 |
| DOI: | 10.3390/toxins13120904 |
| الإتاحة: | https://doi.org/10.3390/toxins13120904Test https://inserm.hal.science/inserm-03516725Test https://inserm.hal.science/inserm-03516725/documentTest https://inserm.hal.science/inserm-03516725/file/toxins-13-00904.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.35BE81D5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/toxins13120904 |
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