التفاصيل البيبلوغرافية
| العنوان: |
Sequence Diversity in tRNA Gene Locus A-L among Iranian Isolates of Entamoeba dispar. |
| المؤلفون: |
Nazemalhosseini-Mojarad, E.1 ehsanmojarad@gmail.com, Azimirad, M.1, Nochi, Z.1, Romani, S.1, Tajbakhsh, M.1, Rostami-Nejad, M.1, Haghighi, A.2, Zali, M. R.1 |
| المصدر: |
Iranian Journal of Parasitology. 2012, Vol. 7 Issue 1, p97-103. 7p. |
| مصطلحات موضوعية: |
*GENETIC research, *AMPLIFIED fragment length polymorphism, *ENTAMOEBA, *ENDAMOEBIDAE, *ENTAMOEBA bovis |
| مصطلحات جغرافية: |
IRAN |
| مستخلص: |
Background: A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was analyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates. Methods: A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR and PCR products were sequenced .The sequences obtained were edited manually and aligned using Gene Runner software. Results: With sequencing of PCR products a reliable genetic diversity in size, number and position of the repeat units were observed among the Iranian E. dispar isolates in locus A-L gene. Sequences showed variation in length from 448bp to 507bp and seven distinct types were identified. Conclusion: The genetic diversity of loci like A-L shows them to be suitable for epidemiological studies such as the characterization of the routes of transmission of these parasites in Iran. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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