SNARE proteins are required for macroautophagy
| العنوان: | SNARE proteins are required for macroautophagy |
|---|---|
| المؤلفون: | Noor Gammoh, Misuzu Baba, Tyler J. Moss, James A. McNew, Thomas J. Melia, Diana N. Richerson, Xuejun Jiang, Janice Griffith, Usha Nair, Ke Wang, Anjali Jotwani, Shanta Nag, Daniel J. Klionsky, Wei Lien Yen, Fulvio Reggiori, Jiefei Geng |
| المصدر: | Nair, U, Jotwani, A, Geng, J, Gammoh, N, Richerson, D, Yen, W-L, Griffith, J, Nag, S, Wang, K, Moss, T, Baba, M, McNew, J A, Jiang, X, Reggiori, F, Melia, T J & Klionsky, D J 2011, ' SNARE proteins are required for macroautophagy ', Cell, vol. 146, no. 2, pp. 290-302 . https://doi.org/10.1016/j.cell.2011.06.022Test Nair, U, Jotwani, A, Geng, J, Noor, G, Richerson, D, Yen, W-L, Griffith, J, Nag, S, Wang, K, Moss, T, Baba, M, McNew, J A, Jiang, X, Reggiori, F, Melia, T J & Klionsky, D J 2011, ' SNARE proteins are required for macroautophagy ', Cell, vol. 146, no. 2, pp. 290-302 . https://doi.org/10.1016/j.cell.2011.06.022Test |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Autophagosome, Phagosomes/metabolism, Autophagy-Related Protein 8 Family, Saccharomyces cerevisiae Proteins, Endosome, ATG8, Saccharomyces cerevisiae/cytology, Qa-SNARE Proteins/metabolism, Autophagy-Related Proteins, Saccharomyces cerevisiae, Biology, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Lysosome, Phagosomes, medicine, Autophagy, 030304 developmental biology, 0303 health sciences, Biochemistry, Genetics and Molecular Biology(all), Qa-SNARE Proteins, Phosphatidylethanolamines, 030302 biochemistry & molecular biology, Membrane Proteins, Phosphatidylethanolamines/metabolism, Saccharomyces cerevisiae Proteins/metabolism, Cell biology, medicine.anatomical_structure, Liposomes/metabolism, Autophagosome membrane, Liposomes, Microtubule-Associated Proteins/metabolism, SNARE Proteins/metabolism, Membrane Proteins/metabolism, SNARE Proteins, Microtubule-Associated Proteins, Biogenesis |
| الوصف: | Summary Macroautophagy mediates the degradation of long-lived proteins and organelles via the de novo formation of double-membrane autophagosomes that sequester cytoplasm and deliver it to the vacuole/lysosome; however, relatively little is known about autophagosome biogenesis. Atg8, a phosphatidylethanolamine-conjugated protein, was previously proposed to function in autophagosome membrane expansion, based on the observation that it mediates liposome tethering and hemifusion in vitro. We show here that with physiological concentrations of phosphatidylethanolamine, Atg8 does not act as a fusogen. Rather, we provide evidence for the involvement of exocytic Q/t-SNAREs in autophagosome formation, acting in the recruitment of key autophagy components to the site of autophagosome formation, and in regulating the organization of Atg9 into tubulovesicular clusters. Additionally, we found that the endosomal Q/t-SNARE Tlg2 and the R/v-SNAREs Sec22 and Ykt6 interact with Sso1-Sec9, and are required for normal Atg9 transport. Thus, multiple SNARE-mediated fusion events are likely to be involved in autophagosome biogenesis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bc3d6b2c149ab46faa83c3e152302dfTest https://pure.au.dk/portal/da/publications/snare-proteins-are-required-for-macroautophagyTest(46e145d4-996c-4a10-af2b-52d7dc54720d).html |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1bc3d6b2c149ab46faa83c3e152302df |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |