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Increased Fatty Acid Oxidation in Differentiated Proximal Tubular Cells Surviving a Reversible Episode of Acute Kidney Injury

التفاصيل البيبلوغرافية
العنوان: Increased Fatty Acid Oxidation in Differentiated Proximal Tubular Cells Surviving a Reversible Episode of Acute Kidney Injury
المؤلفون: Aurélien Bataille, Iman Sadia, Alexandre Hertig, Badreddine Mohand Oumoussa, Marie-Julia Ziliotis, Stéphane Le Crom, Morgane Commereuc, Sophie Vandermeersch, Eric Rondeau, Pierre Galichon, David Legouis, Nadjim Chelghoum, Raphael Cohen, Yi-Chun Xu-Dubois, Noémie Simon-Tillaux
المساهمون: Sorbonne Université (SU), Des Maladies Rénales Rares aux Maladies Fréquentes, Remodelage et Réparation, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Marqueurs cardiovasculaires en situation de stress (MASCOT (UMR_S_942 / U942)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Université Sorbonne Paris Nord, Urgences néphrologiques et transplantation rénale [CHU Tenon], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Plateforme Post-génomique de la Pitié-Salpêtrière (P3S), UMS omique (OMIQUE), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Analyse des Données à Haut Débit en Génomique (ADHDG), Evolution Paris Seine, Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Service d'Urgences néphrologiques et transplantation rénale [CHU Tenon], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), Gestionnaire, Hal Sorbonne Université, AP-HP Hôpital Tenon [Paris], Hôpital Lariboisière, Hôpital Lariboisière-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université de Paris, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris-Université Paris-Sud - Paris 11 (UP11)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Sorbonne Paris Nord, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Tenon [APHP], Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Evolution Paris-Seine, Centre National de la Recherche Scientifique (CNRS)-Université Sorbonne Paris Cité (USPC)-Université des Antilles (UA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Sorbonne Université (SU)-Institut de Recherche pour le Développement (IRD)
المصدر: Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry, Karger, 2018, 47 (4), pp.1338-1351. ⟨10.1159/000490819⟩
Cellular Physiology and Biochemistry, Vol 47, Iss 4, Pp 1338-1351 (2018)
Cellular Physiology and Biochemistry, Vol. 47, No 4 (2018) pp. 1338-1351
Cellular Physiology and Biochemistry, 2018, 47 (4), pp.1338-1351. ⟨10.1159/000490819⟩
بيانات النشر: HAL CCSD, 2018.
سنة النشر: 2018
مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, Physiology, Cell Survival, [SDV]Life Sciences [q-bio], lcsh:Physiology, Epithelium, Kidney Tubules, Proximal, lcsh:Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, Mice, Fibrosis, Internal medicine, AC133 Antigen/metabolism, Chronic Kidney Disease, medicine, Renal fibrosis, Animals, Glycolysis, lcsh:QD415-436, AC133 Antigen, Beta oxidation, Kidney, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Fatty acid metabolism, lcsh:QP1-981, business.industry, Fatty Acids, Acute kidney injury, Acute Kidney Injury/metabolism/pathology, Acute Kidney Injury, medicine.disease, [SDV] Life Sciences [q-bio], Fatty Acids/metabolism, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, chemistry, business, Fatty Acid Oxidation, Oxidation-Reduction, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Kidney disease, Kidney Tubules, Proximal/metabolism/pathology
الوصف: International audience; Background/aims: Fatty acid oxidation (FAO), the main source of energy produced by tubular epithelial cells in the kidney, was found to be defective in tubulo-interstitial samples dissected out in kidney biopsies from patients with chronic kidney disease (CKD). Experimental data indicated that this decrease was a strong determinant of renal fibrogenesis, hence a focus for therapeutic interventions. Nevertheless, whether persistently differentiated renal tubules, surviving in a pro-fibrotic environment, also suffer from a decrease in FAO, is currently unknown.Methods: To address this question, we isolated proximal tubules captured ex vivo on the basis of the expression of an intact brush border antigen (Prominin-1) in C57BL6/J mice subjected to a controlled, two-hit model of renal fibrosis (reversible ischemic acute kidney injury (AKI) or sham surgery, followed by angiotensin 2 administration). A transcriptomic high throughput sequencing was performed on total mRNA from these cells, and on whole kidneys.Results: In contrast to mice subjected to sham surgery, mice with a history of AKI displayed histologically more renal fibrosis when exposed to angiotensin 2. High throughput RNA sequencing, principal component analysis and clustering showed marked consistency within experimental groups. As expected, FAO transcripts were decreased in whole fibrotic kidneys. Surprisingly, however, up- rather than down-regulation of metabolic pathways (oxidative phosphorylation, fatty acid metabolism, glycolysis, and PPAR signalling pathway) was a hallmark of the differentiated tubules captured from fibrotic kidneys. Immunofluorescence co-staining analysis confirmed that the expression of FAO enzymes was dependent of tubular trophicity.Conclusions: These data suggest that in differentiated proximal tubules energetic hyperactivity is promoted concurrently with organ fibrogenesis.
وصف الملف: application/pdf
اللغة: English
تدمد: 1015-8987
1421-9778
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffeb4a3671bfd1c8aced574525fcb009Test
https://hal.sorbonne-universite.fr/hal-02565135/documentTest
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....ffeb4a3671bfd1c8aced574525fcb009
قاعدة البيانات: OpenAIRE
الوصف
تدمد:10158987
14219778