المؤلفون: Haixue Kuang, Ji Li, Nannan Xing, Pengling Ge, Dongwei Han, Lei Zhu

المصدر: BioMed Research International, Vol 2015 (2015)
BioMed Research International

مصطلحات موضوعية: medicine.medical_specialty, Vinyl Compounds, Article Subject, endocrine system diseases, media_common.quotation_subject, Panax, lcsh:Medicine, Ovary, 12E7 Antigen, Primary Ovarian Insufficiency, Dinoprostone, General Biochemistry, Genetics and Molecular Biology, Follicle-stimulating hormone, Western blot, Antigens, CD, Internal medicine, Cyclohexenes, medicine, Animals, Humans, Ovulation, American ginseng, media_common, General Immunology and Microbiology, medicine.diagnostic_test, biology, Plant Extracts, lcsh:R, General Medicine, Luteinizing Hormone, medicine.disease, biology.organism_classification, female genital diseases and pregnancy complications, Rats, Premature ovarian failure, medicine.anatomical_structure, Endocrinology, Gene Expression Regulation, Female, Follicle Stimulating Hormone, Human, Luteinizing hormone, Cell Adhesion Molecules, Research Article, Hormone

الوصف: Premature ovarian failure (POF) is defined as lost ovarian functions before the age of 40. Three possible molecular markers (PLA2G4A,miR-29a, andmiR-144) have been identified in our previous study by integrated analysis of mRNA and miRNA expression profiles. The present study aimed to evaluate American ginseng root’s protective potential against POF by studying transcriptional and protein variations between American ginseng treatments and controls in rats. 4-Vinylcyclohexene diepoxide (VCD) was administered to rats for 14 days to induce POF. Additionally, American ginseng was administered to POF rats for one month, andPLA2G4A,miR-29a, andmiR-144expressions were measured in rat ovaries by qRT-PCR. PLA2G4A protein expression was examined by Western Blot, and PGE2, LH, FSH, and E2 serum levels were detected by ELISA.PLA2G4AmRNA and protein were downregulated in American ginseng-treated rats,miR-29aandmiR-144levels increased, and PGE2serum levels decreased, while LH, FSH, and E2 increased compared to POF induction alone. Analysis of transcriptional and protein variations suggested that American ginseng protects the ovary against POF by regulating prostaglandin biosynthesis, ovulation, and preventing ovarian aging. High hormone levels (PGE2, FSH, and LH) were reduced, and E2 secretion approached normal levels, leading to improved POF symptoms and abnormal ovulation.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aff4adfb6eb81bd8a4f9655655b0d263Test
https://doi.org/10.1155/2015/767124Test

المؤلفون: Pengling Ge, Nannan Xing, Ji Li, Yanhai Ren, Dongwei Han, Haixue Kuang, Lei Zhu

المصدر: Drug Development Research. 75:521-528

مصطلحات موضوعية: medicine.medical_specialty, endocrine system diseases, biology, business.industry, H&E stain, CCL2, biology.organism_classification, medicine.disease, Endometrium, PLA2G4A, female genital diseases and pregnancy complications, Premature ovarian failure, Ginseng, Endocrinology, medicine.anatomical_structure, Internal medicine, Drug Discovery, medicine, biology.protein, business, American ginseng, Hormone

الوصف: Preclinical Research Premature ovarian failure (POF) is defined by the WHO as the loss of physiological ovarian function before the age of 40. The effect of American ginseng and its underlying mechanisms in preventing and treating premature ovarian failure (POF) was studied in female Sprague-Dawley rats where POF was induced by ip administration of 4-vinylcyclohexene diepoxide (VCD). Rat behavior, serum hormone levels, ovarian and uterine size, pathological features, and ovarian tissue expression of genes associated with POF were assessed in controls, untreated POF model rats, and POF model rats treated with low- (1.125 g/kg), medium- (2.25 g/kg), and high-dose (4.5 g/kg) American ginseng. Compared with untreated POF model rats, those treated with medium- and high-dose American ginseng had more stable behavior and better coat appearance as well as serum hormone levels closer to those in control rats. Moreover, treatment with medium- or high-dose American ginseng increased ovarian and uterine size. Hematoxylin and eosin-staining revealed mature follicles and endometrium with an alternating concave/convex surface structure with visible capillaries and glands in ginseng- treated POF rats. PLA2G4A expression was positively correlated with POF, while the expression levels of PAPPA, STC2, CCL2, and NELL1 were negatively correlated with POF. Our study showed that American ginseng may effectively prevent POF and alleviate POF symptoms by regulating serum hormone levels and altering the expression levels of genes related to POF in ovarian tissue.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::35f278b3c5ee5c347710bbbc28f3db3bTest
https://doi.org/10.1002/ddr.21234Test

المؤلفون: Jiaming Xie, Ji Li, Pengling Ge, Dongwei Han, Shengjun Fan, Haixue Kuang

المصدر: Experimental and molecular pathology. 97(3)

مصطلحات موضوعية: endocrine system diseases, Clinical Biochemistry, Enzyme-Linked Immunosorbent Assay, Biology, Primary Ovarian Insufficiency, Pathology and Forensic Medicine, Rats, Sprague-Dawley, Complementary DNA, Gene expression, medicine, Animals, Cluster Analysis, Gene Regulatory Networks, RNA, Messenger, Molecular Biology, Transcription factor, Gene, Oligonucleotide Array Sequence Analysis, Genetics, Messenger RNA, Principal Component Analysis, Gene Expression Profiling, Computational Biology, medicine.disease, female genital diseases and pregnancy complications, Premature ovarian failure, Rats, Gene expression profiling, Disease Models, Animal, Female, Signal transduction

الوصف: Premature ovarian failure (POF) remains one of the major gynecological problems worldwide which affected 1% of women. Even though tremendous achievements had been acquired as opposed to years past, molecular pathogenesis associated with POF is still unclear and needs to be well-defined. The aim of this study was to analyze the gene expression profiles in the POF rat model. To predict potential regulating factors, we firstly treated female Sprague Dawley (SD) rat with 4-vinylcyclohexene diepoxide (VCD). Total RNA from ovarian tissue was converted to cDNA and hybridized to mRNA Chip array. The differentially expressed genes (DEGs) were identified by two-sample t test and assessed using hierarchical clustering and Principal Component Analysis methods. Potential regulatory targets associated with these DEGs were constructed using BisoGenet in Cytoscape. Gene Ontology (GO) and functional enrichment analysis were performed using BiNGO and DAVID, respectively. As the results, 25 DEGs were found to be closely associated with POF initiation. Hierarchical clustering and Principal Component Analysis on the transcriptional profiles revealed an excellent separation of the vehicle and POF compartments. Pathway enrichment analysis based on the disease-gene interaction network analysis led to the identification of two core signaling pathways that were strongly affected during POF initiation and progression: immune response and cardiovascular disorders. In conclusion, we constructed a gene regulatory network associated with POF using the microarray gene expression profiling, and screened out some genes or transcription factors that may be used as potential molecular therapeutic targets for POF.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee4e7e6e84aa552ddf9841447d66c95aTest
https://pubmed.ncbi.nlm.nih.gov/25445499Test

المؤلفون: Haixue Kuang, Pengling Ge, Ji Li, Jiaming Xie, Dongwei Han, Yongxin Yan

المصدر: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology. 30(1)

مصطلحات موضوعية: endocrine system diseases, Endocrinology, Diabetes and Metabolism, CCL2, Primary Ovarian Insufficiency, Bioinformatics, Rats, Sprague-Dawley, Endocrinology, microRNA, medicine, Animals, Humans, Gene Regulatory Networks, biology, Microarray analysis techniques, Gene Expression Profiling, Obstetrics and Gynecology, medicine.disease, Microarray Analysis, PLA2G4A, female genital diseases and pregnancy complications, Premature ovarian failure, Rats, Gene expression profiling, Disease Models, Animal, MicroRNAs, Cancer research, biology.protein, Female, DNA microarray, Hormone

الوصف: Premature ovarian failure (POF) contributes to amenorrhoea, infertility, early onset of menostasia and osteoporosis. This study profiled differentially expressed miRNAs for association with POF development. Ovarian tissue samples from 4-vinylcyclohexene diepoxide (VCD)-induced rat POF and normal rats were profiled for differentially expressed miRNAs using miRNA microarrays. A total of 63 miRNAs were up-regulated and 20 miRNAs were down-regulated in rat POF tissues versus the control tissues. qRT-PCR verified some of these altered miRNAs, i.e. miR-29a and miR-144 were down-regulated in POF tissues, which may target expression of PLA2G4A that is involved in prostaglandin biosynthesis, whereas miR-27b and miR-190 were up-regulated in POF tissues by negative control of PAPPA and CCL2 expression, respectively, both of which have been shown to relate to response to hormone stimulus. Moreover, the up-regulated miR-151 and miR-672 can also target expression of TNFSF10 and FNDC1, which have been shown to positively regulate cell apoptosis. Profiling of differentially expressed miRNAs in POF provided a novel insight into the molecular events involving the role of miRNAs in POF development with specific emphasis upon miR-27b, miR-190, miR-151, miR-672, miR-29a and miR-144.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44ef5eba10a988bf3bea1a700102d234Test
https://pubmed.ncbi.nlm.nih.gov/24188450Test