دورية أكاديمية
MiR-205 targets angiogenesis and EMT concurrently in anaplastic thyroid carcinoma
| العنوان: | MiR-205 targets angiogenesis and EMT concurrently in anaplastic thyroid carcinoma |
|---|---|
| المؤلفون: | Vosgha, Haleh, Ariana, Armin, Smith, Robert Anthony, Lam, Alfred King Yin |
| المصدر: | Endocrine-Related Cancer |
| بيانات النشر: | BioScientifica Ltd. |
| سنة النشر: | 2018 |
| المجموعة: | Queensland University of Technology: QUT ePrints |
| مصطلحات موضوعية: | Anaplastic thyroid carcinoma, Angiogenesis, EMT, Invasion, miRNA-205 |
| الوصف: | The current study aims to evaluate for the first time the inhibitory roles of miR-205 in the pathogenesis of anaplastic thyroid carcinoma. In addition, we investigated the mechanisms by which miR-205 regulates angiogenesis and epithelial-to-mesenchymal transition (EMT) in cancer. Two anaplastic thyroid carcinoma cell lines were transfected with the expression vector pCMV-MIR-205. Selected markers of angiogenesis and EMT including vascular endothelial growth factor A (VEGF-A) and zinc finger E-box-binding homeobox 1 (ZEB1) were investigated by Western blot. The interaction of miR-205 expression with EMT and angiogenesis were also investigated by assessment of matrix metalloproteinases 2 and 9 (MMP2 and MMP 9), SNAI1 (Snai1 family zinc finger 1), vimentin, E-cadherin and N-cadherin. The function of miR-205 was further tested with VEGF enzyme-linked immunosorbent assay (ELISA), wound healing, invasion and tube formation assays. Using an animal model, we studied the association of miR-205 with angiogenesis, proliferation and invasion. The following results were obtained. Permanent overexpression of miR-205 significantly suppressed angiogenesis and EMT by simultaneously targeting VEGF-A, ZEB1 and downstream products. Ectopic expression of miR-205 in cancer cells led to decreased migration, invasion and tube formation of endothelial cells. In addition, inhibition of tumour growth, vascularisation and invasion were noted in the mouse tumour xenografts. Our findings provide insights into simultaneous regulatory role of miR-205 in the pathogenesis of anaplastic thyroid carcinoma by suppressing both angiogenesis and EMT. This may open avenues to exploit miR-205 as an alternative cancer therapeutic strategy in the future. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | Vosgha, Haleh, Ariana, Armin, Smith, Robert Anthony, & Lam, Alfred King Yin (2018) MiR-205 targets angiogenesis and EMT concurrently in anaplastic thyroid carcinoma. Endocrine-Related Cancer, 25(3), pp. 323-337.; https://eprints.qut.edu.au/229785Test/; Institute of Health and Biomedical Innovation |
| الإتاحة: | https://doi.org/10.1530/ERC-17-0497Test https://eprints.qut.edu.au/229785Test/ |
| حقوق: | © 2018 Society for Endocrinology ; This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
| رقم الانضمام: | edsbas.E26E1722 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |