التفاصيل البيبلوغرافية
| العنوان: |
Distinct functions and temporal regulation of methylated histone H3 during early embryogenesis. |
| المؤلفون: |
Mutlu, Beste, Huei-Mei Chen, Gutnik, Silvia, Hall, David H., Keppler-Ross, Sabine, Mango, Susan E. |
| المصدر: |
Development (09501991); Oct2019, Vol. 146 Issue 19, p1-46, 46p |
| مصطلحات موضوعية: |
EMBRYOLOGY, HETEROCHROMATIN, CELL cycle, HISTONES, CELL cycle regulation, METHYLTRANSFERASES, EMBRYOS |
| مستخلص: |
During the first hours of embryogenesis, formation of higher-order heterochromatin coincides with the loss of developmental potential. Here we examine the relationship between these two events, and we probe the processes that contribute to the timing of their onset. Mutations that disrupt histone H3 lysine 9 (H3K9) methyltransferases reveal that the methyltransferase MET-2 helps terminate developmental plasticity, through mono- and di- methylation of H3K9 (me1/me2), and promotes heterochromatin formation, through H3K9me3. While loss of H3K9me3 perturbs formation of higher-order heterochromatin, embryos are still able to terminate plasticity, indicating that the two processes can be uncoupled. Methylated H3K9 appears gradually in developing embryos and depends on nuclear localization of MET- 2. We find that the timing of H3K9me2 and nuclear MET-2 is sensitive to rapid cell cycles, but not to zygotic genome activation or cell counting. These data reveal distinct roles for different H3K9 methylation states in the generation of heterochromatin and loss of developmental plasticity by MET-2 and identify the cell cycle as a critical parameter of MET-2 regulation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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