التفاصيل البيبلوغرافية
| العنوان: |
Intrauterine infusion of low levels of interferon‐tau on day‐8 post‐estrus stimulates the bovine endometrium to secrete apolipoprotein‐A1: A possible implication for early embryo tolerance. |
| المؤلفون: |
Rashid, Mohammad B., Marey, Mohamed A., Fukuda, Kenji, Haneda, Shingo, Kusama, Kazuya, Shimada, Masayuki, Imakawa, Kazuhiko, Miyamoto, Akio |
| المصدر: |
American Journal of Reproductive Immunology; Sep2022, Vol. 88 Issue 3, p1-14, 14p |
| مصطلحات موضوعية: |
MONONUCLEAR leukocytes, BOS, EMBRYOS, ENDOMETRIUM, TRANSFORMING growth factors-beta |
| مستخلص: |
We previously reported that interferon‐tau (IFNT), derived from day‐7 blastocyst, generates anti‐inflammatory responses in bovine endometrial epithelial cells (BEECs) in vitro. However, the real in vivo impact of early embryo‐derived IFNT on the uterine proteomic profile is mostly unknown. This study aimed to investigate proteomic changes of uterine flush (UF) when infused with a low physiological level of IFNT without embryo on day‐8 post‐estrus and its possible impact on the uterine immunological microenvironment. First, a fresh medium was infused into the uterine lumen on day‐6, from which UF was obtained 24 h later, and this procedure was repeated on day‐7 (control UF). On day‐8, this procedure was done with a medium containing recombinant bovine IFNT (100 pg/ml) (IFNT‐supplemented UF). Control and IFNT‐supplemented UF were tested for immune responses in peripheral blood mononuclear cells (PBMCs). Real‐time PCR results revealed that IFNT‐supplemented UF downregulated pro‐inflammatory cytokines (TNFA, IL1B) and upregulated anti‐inflammatory cytokine (TGFB1) and PTGES in PBMCs. Through 2‐D PAGE, followed by TOF/TOF mass spectrometer, apolipoprotein‐A1 (Apo‐A1) protein was identified in the IFNT‐supplemented UF, which was confirmed by ELISA analysis. Proteomic analysis revealed again that the in vitro stimulation of BEECs by IFNT upregulated Apo‐A1 expression. Further, stimulation of PBMCs with recombinant bovine Apo‐A1 downregulated TNFA and NFKB and upregulated TGFB1 and PTGES in PBMCs. Altogether, our results suggest that minute amounts of IFNT alone, normally secreted from bovine blastocyst, stimulate Apo‐A1 secretion from the endometrial epithelium in the absence of embryo that initiates an anti‐inflammatory environment, which could pave the way for the acceptance of early embryo in the uterus. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
