المؤلفون: Normand, Anne-Cécile, Imbert, Sébastien, Brun, Sophie, Al-Hatmi, Abdullah, M S, Chryssanthou, Erja, Cassaing, Sophie, Schuttler, Christine, Hasseine, Lilia, Mahinc, Caroline, Costa, Damien, Bonnal, Christine, Ranque, Stephane, Sautour, Marc, Rubio, Elisa, Delhaes, Laurence, Riat, Arnaud, Sendid, Boualem, Kristensen, Lise, Brandenberger, Marcel, Guitard, Juliette, Packeu, Ann, Piarroux, Renaud, Fekkar, Arnaud

المساهمون: CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Avicenne AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Radboud University Medical Center Nijmegen, Karolinska Institutet Stockholm, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre Hospitalier Universitaire de Nice (CHU Nice), Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E), CHU Rouen, Normandie Université (NU), CIC - CHU Bichat, Institut National de la Santé et de la Recherche Médicale (INSERM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées Brétigny-sur-Orge (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), University of Barcelona, Instituto de Salud Global - Institute For Global Health Barcelona (ISGlobal), CHU de Bordeaux Pellegrin Bordeaux, Geneva University Hospitals and Geneva University, Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Aarhus University Hospital, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Saint-Antoine AP-HP, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 2309-608X ; Journal of Fungi ; https://hal.sorbonne-universite.fr/hal-03184998Test ; Journal of Fungi, 2021, 7 (4), pp.246. ⟨10.3390/jof7040246⟩.

مصطلحات موضوعية: fusariosis, mALDI ToF mass spectrometry, DNA sequencing, elongation factor, Fusarium species complex, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

العلاقة: hal-03184998; https://hal.sorbonne-universite.fr/hal-03184998Test; https://hal.sorbonne-universite.fr/hal-03184998/documentTest; https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

الإتاحة: https://doi.org/10.3390/jof7040246Test
https://hal.sorbonne-universite.fr/hal-03184998Test
https://hal.sorbonne-universite.fr/hal-03184998/documentTest
https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

المؤلفون: Normand, Anne-Cécile, Imbert, Sébastien, Brun, Sophie, Al-Hatmi, Abdullah, M S, Chryssanthou, Erja, Cassaing, Sophie, Schuttler, Christine, Hasseine, Lilia, Mahinc, Caroline, Costa, Damien, Bonnal, Christine, Ranque, Stephane, Sautour, Marc, Rubio, Elisa, Delhaes, Laurence, Riat, Arnaud, Sendid, Boualem, Kristensen, Lise, Brandenberger, Marcel, Guitard, Juliette, Packeu, Ann, Piarroux, Renaud, Fekkar, Arnaud

المساهمون: CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Avicenne AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Radboud University Medical Center Nijmegen, Karolinska Institutet Stockholm, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre Hospitalier Universitaire de Nice (CHU Nice), Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E), CHU Rouen, Normandie Université (NU), CIC - CHU Bichat, Institut National de la Santé et de la Recherche Médicale (INSERM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées Brétigny-sur-Orge (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), University of Barcelona, Instituto de Salud Global - Institute For Global Health Barcelona (ISGlobal), CHU de Bordeaux Pellegrin Bordeaux, Geneva University Hospitals and Geneva University, Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Université de Lille, Aarhus University Hospital, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Saint-Antoine AP-HP, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 2309-608X ; Journal of Fungi ; https://hal.sorbonne-universite.fr/hal-03184998Test ; Journal of Fungi, 2021, 7 (4), pp.246. ⟨10.3390/jof7040246⟩.

مصطلحات موضوعية: fusariosis, mALDI ToF mass spectrometry, DNA sequencing, elongation factor, Fusarium species complex, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

العلاقة: hal-03184998; https://hal.sorbonne-universite.fr/hal-03184998Test; https://hal.sorbonne-universite.fr/hal-03184998/documentTest; https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

الإتاحة: https://doi.org/10.3390/jof7040246Test
https://hal.sorbonne-universite.fr/hal-03184998Test
https://hal.sorbonne-universite.fr/hal-03184998/documentTest
https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

المؤلفون: Normand, Anne-Cécile, Imbert, Sébastien, Brun, Sophie, Al-Hatmi, Abdullah, M S, Chryssanthou, Erja, Cassaing, Sophie, Schuttler, Christine, Hasseine, Lilia, Mahinc, Caroline, Costa, Damien, Bonnal, Christine, Ranque, Stephane, Sautour, Marc, Rubio, Elisa, Delhaes, Laurence, Riat, Arnaud, Sendid, Boualem, Kristensen, Lise, Brandenberger, Marcel, Guitard, Juliette, Packeu, Ann, Piarroux, Renaud, Fekkar, Arnaud

المساهمون: CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Avicenne AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Radboud University Medical Center Nijmegen, Karolinska Institutet Stockholm, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre Hospitalier Universitaire de Nice (CHU Nice), Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E), CHU Rouen, Normandie Université (NU), CIC - CHU Bichat, Institut National de la Santé et de la Recherche Médicale (INSERM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées Brétigny-sur-Orge (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), University of Barcelona, Instituto de Salud Global - Institute For Global Health Barcelona (ISGlobal), CHU de Bordeaux Pellegrin Bordeaux, Geneva University Hospitals and Geneva University, Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Aarhus University Hospital, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Saint-Antoine AP-HP, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 2309-608X ; Journal of Fungi ; https://hal.sorbonne-universite.fr/hal-03184998Test ; Journal of Fungi, 2021, 7 (4), pp.246. ⟨10.3390/jof7040246⟩.

مصطلحات موضوعية: fusariosis, mALDI ToF mass spectrometry, DNA sequencing, elongation factor, Fusarium species complex, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

العلاقة: hal-03184998; https://hal.sorbonne-universite.fr/hal-03184998Test; https://hal.sorbonne-universite.fr/hal-03184998/documentTest; https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

الإتاحة: https://doi.org/10.3390/jof7040246Test
https://hal.sorbonne-universite.fr/hal-03184998Test
https://hal.sorbonne-universite.fr/hal-03184998/documentTest
https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

المؤلفون: Normand, Anne-Cécile, Imbert, Sébastien, Brun, Sophie, Al-Hatmi, Abdullah, M S, Chryssanthou, Erja, Cassaing, Sophie, Schuttler, Christine, Hasseine, Lilia, Mahinc, Caroline, Costa, Damien, Bonnal, Christine, Ranque, Stephane, Sautour, Marc, Rubio, Elisa, Delhaes, Laurence, Riat, Arnaud, Sendid, Boualem, Kristensen, Lise, Brandenberger, Marcel, Guitard, Juliette, Packeu, Ann, Piarroux, Renaud, Fekkar, Arnaud

المساهمون: CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Avicenne AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Radboud University Medical Center Nijmegen, Karolinska Institutet Stockholm, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre Hospitalier Universitaire de Nice (CHU Nice), Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E), CHU Rouen, Normandie Université (NU), CIC - CHU Bichat, Institut National de la Santé et de la Recherche Médicale (INSERM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées Brétigny-sur-Orge (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), University of Barcelona, Instituto de Salud Global - Institute For Global Health Barcelona (ISGlobal), CHU de Bordeaux Pellegrin Bordeaux, Geneva University Hospitals and Geneva University, Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Aarhus University Hospital, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Saint-Antoine AP-HP, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 2309-608X ; Journal of Fungi ; https://hal.sorbonne-universite.fr/hal-03184998Test ; Journal of Fungi, 2021, 7 (4), pp.246. ⟨10.3390/jof7040246⟩.

مصطلحات موضوعية: fusariosis, mALDI ToF mass spectrometry, DNA sequencing, elongation factor, Fusarium species complex, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

العلاقة: hal-03184998; https://hal.sorbonne-universite.fr/hal-03184998Test; https://hal.sorbonne-universite.fr/hal-03184998/documentTest; https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

الإتاحة: https://doi.org/10.3390/jof7040246Test
https://hal.sorbonne-universite.fr/hal-03184998Test
https://hal.sorbonne-universite.fr/hal-03184998/documentTest
https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

المؤلفون: Normand, Anne Cécile, Imbert, Sébastien, Brun, Sophie, Al-Hatmi, Abdullah M.S., Chryssanthou, Erja, Cassaing, Sophie, Schuttler, Christine, Hasseine, Lilia, Mahinc, Caroline, Costa, Damien, Bonnal, Christine, Ranque, Stéphane, Sautour, Marc, Rubio, Elisa, Delhaes, Laurence, Riat, Arnaud, Sendid, Boualem, Kristensen, Lise, Brandenberger, Marcel, Guitard, Juliette, Packeu, Ann, Piarroux, Renaud, Fekkar, Arnaud

المصدر: Normand , A C , Imbert , S , Brun , S , Al-Hatmi , A M S , Chryssanthou , E , Cassaing , S , Schuttler , C , Hasseine , L , Mahinc , C , Costa , D , Bonnal , C , Ranque , S , Sautour , M , Rubio , E , Delhaes , L , Riat , A , Sendid , B , Kristensen , L , Brandenberger , M , Guitard , J , Packeu , A , Piarroux , R & Fekkar , A 2021 , ' Clinical origin ....

مصطلحات موضوعية: DNA sequencing, Elongation factor, Fusariosis, Fusarium species complex, MALDI ToF mass spectrometry

الوصف: Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

العلاقة: https://pure.au.dk/portal/en/publications/c49f90e9-646f-495f-976f-c89acf6bcf2cTest

الإتاحة: https://doi.org/10.3390/jof7040246Test
https://pure.au.dk/portal/en/publications/c49f90e9-646f-495f-976f-c89acf6bcf2cTest
http://www.scopus.com/inward/record.url?scp=85103961583&partnerID=8YFLogxKTest

المؤلفون: Normand, Anne-Cécile, Imbert, Sébastien, Brun, Sophie, Al-Hatmi, Abdullah, M S, Chryssanthou, Erja, Cassaing, Sophie, Schuttler, Christine, Hasseine, Lilia, Mahinc, Caroline, Costa, Damien, Bonnal, Christine, Ranque, Stephane, Sautour, Marc, Rubio, Elisa, Delhaes, Laurence, Riat, Arnaud, Sendid, Boualem, Kristensen, Lise, Brandenberger, Marcel, Guitard, Juliette, Packeu, Ann, Piarroux, Renaud, Fekkar, Arnaud

المساهمون: CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Avicenne AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Radboud University Medical Center Nijmegen, Karolinska Institutet Stockholm, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre Hospitalier Universitaire de Nice (CHU Nice), Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E), CHU Rouen, Normandie Université (NU), CIC - CHU Bichat, Institut National de la Santé et de la Recherche Médicale (INSERM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées Brétigny-sur-Orge (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), University of Barcelona, Instituto de Salud Global - Institute For Global Health Barcelona (ISGlobal), CHU de Bordeaux Pellegrin Bordeaux, Geneva University Hospitals and Geneva University, Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Aarhus University Hospital, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Saint-Antoine AP-HP, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 2309-608X ; Journal of Fungi ; https://hal.sorbonne-universite.fr/hal-03184998Test ; Journal of Fungi, 2021, 7 (4), pp.246. ⟨10.3390/jof7040246⟩.

مصطلحات موضوعية: fusariosis, mALDI ToF mass spectrometry, DNA sequencing, elongation factor, Fusarium species complex, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

العلاقة: hal-03184998; https://hal.sorbonne-universite.fr/hal-03184998Test; https://hal.sorbonne-universite.fr/hal-03184998/documentTest; https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

الإتاحة: https://doi.org/10.3390/jof7040246Test
https://hal.sorbonne-universite.fr/hal-03184998Test
https://hal.sorbonne-universite.fr/hal-03184998/documentTest
https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

المؤلفون: Normand, Anne-Cécile, Imbert, Sébastien, Brun, Sophie, Al-Hatmi, Abdullah, M S, Chryssanthou, Erja, Cassaing, Sophie, Schuttler, Christine, Hasseine, Lilia, Mahinc, Caroline, Costa, Damien, Bonnal, Christine, Ranque, Stephane, Sautour, Marc, Rubio, Elisa, Delhaes, Laurence, Riat, Arnaud, Sendid, Boualem, Kristensen, Lise, Brandenberger, Marcel, Guitard, Juliette, Packeu, Ann, Piarroux, Renaud, Fekkar, Arnaud

المساهمون: CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Avicenne AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Radboud University Medical Center Nijmegen, Karolinska Institutet Stockholm, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre Hospitalier Universitaire de Nice (CHU Nice), Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E), CHU Rouen, Normandie Université (NU), CIC - CHU Bichat, Institut National de la Santé et de la Recherche Médicale (INSERM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées Brétigny-sur-Orge (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), University of Barcelona, Instituto de Salud Global - Institute For Global Health Barcelona (ISGlobal), CHU de Bordeaux Pellegrin Bordeaux, Geneva University Hospitals and Geneva University, Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Aarhus University Hospital, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Saint-Antoine AP-HP, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 2309-608X ; Journal of Fungi ; https://hal.sorbonne-universite.fr/hal-03184998Test ; Journal of Fungi, 2021, 7 (4), pp.246. ⟨10.3390/jof7040246⟩.

مصطلحات موضوعية: fusariosis, mALDI ToF mass spectrometry, DNA sequencing, elongation factor, Fusarium species complex, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

العلاقة: hal-03184998; https://hal.sorbonne-universite.fr/hal-03184998Test; https://hal.sorbonne-universite.fr/hal-03184998/documentTest; https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

الإتاحة: https://doi.org/10.3390/jof7040246Test
https://hal.sorbonne-universite.fr/hal-03184998Test
https://hal.sorbonne-universite.fr/hal-03184998/documentTest
https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

المؤلفون: Normand, Anne-Cécile, Imbert, Sébastien, Brun, Sophie, Al-Hatmi, Abdullah, Chryssanthou, Erja, Cassaing, Sophie, Schuttler, Christine, Hasseine, Lilia, Mahinc, Caroline, Costa, Damien, Bonnal, Christine, Ranque, Stephane, Sautour, Marc, Rubio, Elisa, Delhaes, Laurence, Riat, Arnaud, Sendid, Boualem, Kristensen, Lise, Brandenberger, Marcel, Guitard, Juliette, Packeu, Ann, Piarroux, Renaud, Fekkar, Arnaud

المساهمون: Service de parasitologie - mycologie CHU Pitié-Salpétrière, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière AP-HP, Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Avicenne AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Radboud University Medical Centre Nijmegen, The Netherlands, Karolinska Institutet Stockholm, CHU Toulouse Toulouse, Centre Hospitalier Universitaire de Nice (CHU Nice), Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), CHU Rouen, Normandie Université (NU), CIC - CHU Bichat, Institut National de la Santé et de la Recherche Médicale (INSERM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), University of Barcelona, Instituto de Salud Global - Institute For Global Health Barcelona (ISGlobal), CHU de Bordeaux Pellegrin Bordeaux, Geneva University Hospitals and Geneva University, CHU Lille, Aarhus University Hospital, Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de Parasitologie - Mycologie CHU Saint-Antoine, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre d'Immunologie et de Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 2309-608X ; Journal of Fungi ; https://hal.sorbonne-universite.fr/hal-03184998Test ; Journal of Fungi, MDPI, 2021, 7 (4), pp.246. ⟨10.3390/jof7040246⟩.

مصطلحات موضوعية: fusariosis, mALDI ToF mass spectrometry, DNA sequencing, elongation factor, Fusarium species complex, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

العلاقة: hal-03184998; https://hal.sorbonne-universite.fr/hal-03184998Test; https://hal.sorbonne-universite.fr/hal-03184998/documentTest; https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest

الإتاحة: https://doi.org/10.3390/jof7040246Test
https://hal.sorbonne-universite.fr/hal-03184998Test
https://hal.sorbonne-universite.fr/hal-03184998/documentTest
https://hal.sorbonne-universite.fr/hal-03184998/file/jof-07-00246-v2.pdfTest