Evaluation of high-resolution mass spectrometry for the quantitative analysis of mycotoxins in complex feed matrices
| العنوان: | Evaluation of high-resolution mass spectrometry for the quantitative analysis of mycotoxins in complex feed matrices |
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| المؤلفون: | Nils Preußke, Sarah De Saeger, Marthe De Boevre, Tim Birr, Joseph-Alexander Verreet, Tolke Jensen, Frank D. Sönnichsen |
| المصدر: | TOXINS Toxins Volume 11 Issue 9 Toxins, Vol 11, Iss 9, p 531 (2019) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | LC-HRMS, ELECTROSPRAY-IONIZATION, Health, Toxicology and Mutagenesis, lcsh:Medicine, Toxicology, 01 natural sciences, METABOLITES, Mass Spectrometry, chemistry.chemical_compound, Fusarium, MULTIPLE, Mathematics, validation, Silage, forage maize, biology, Orbitrap (TM), article, OrbitrapTM, 04 agricultural and veterinary sciences, 040401 food science, LC-MS/MS METHOD, Chemistry, ddc:540, PESTICIDE-RESIDUES, CHROMATOGRAPHY, Food Contamination, Forage, Mass spectrometry, FUSARIUM TOXINS, Zea mays, Article, 0404 agricultural biotechnology, RESOLVING POWER, Mycotoxin, ddc:5, Orbitraptm, Chromatography, Pesticide residue, MYCOTOXINS, 010401 analytical chemistry, lcsh:R, Biology and Life Sciences, Mycotoxins, PERFORMANCE, biology.organism_classification, 0104 chemical sciences, chemistry, Quantitative analysis (chemistry), maize silage, Decision limit, Chromatography, Liquid |
| الوصف: | The selective and sensitive analysis of mycotoxins in highly complex feed matrices is a great challenge. In this study, the suitability of OrbitrapTM-based high-resolution mass spectrometry (HRMS) for routine mycotoxin analysis in complex feeds was demonstrated by the successful validation of a full MS/data-dependent MS/MS acquisition method for the quantitative determination of eight Fusarium mycotoxins in forage maize and maize silage according to the Commission Decision 2002/657/EC. The required resolving power for accurate mass assignments (< 5 ppm) was determined as 35,000 full width at half maximum (FWHM) and 70,000 FWHM for forage maize and maize silage, respectively. The recovery (RA), intra-day precision (RSDr), and inter-day precision (RSDR) of measurements were in the range of 94 to 108%, 2 to 16%, and 2 to 12%, whereas the decision limit (CC&alpha ) and the detection capability (CC&beta ) varied from 11 to 88 µ g/kg and 20 to 141 µ g/kg, respectively. A set of naturally contaminated forage maize and maize silage samples collected in northern Germany in 2017 was analyzed to confirm the applicability of the HRMS method to real samples. At least four Fusarium mycotoxins were quantified in each sample, highlighting the frequent co-occurrence of mycotoxins in feed. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6651 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02fef6a6e4cae22da1c586e694a6991bTest https://hdl.handle.net/1854/LU-8646519Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....02fef6a6e4cae22da1c586e694a6991b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20726651 |
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