التفاصيل البيبلوغرافية
| العنوان: |
A Temperature-Sensitive Recombinant of Avian Coronavirus Infectious Bronchitis Virus Provides Complete Protection against Homologous Challenge. |
| المؤلفون: |
Keep, Sarah1, Stevenson-Leggett, Phoebe1, Dowgier, Giulia1,2, Foldes, Katalin1, Webb, Isobel1, Fones, Albert1, Littolff, Kieran1, Everest, Holly1, Britton, Paul1, Bickerton, Erica1 sarah.keep@pirbright.ac.uk |
| المصدر: |
Journal of Virology. Sep2022, Vol. 96 Issue 17, p1-21. 21p. |
| مصطلحات موضوعية: |
*AVIAN infectious bronchitis virus, *CORONAVIRUSES, *COVID-19, *EGGS, *POULTRY diseases, *COVID-19 vaccines, *VACCINE development |
| مستخلص: |
Avian coronavirus infectious bronchitis virus (IBV) is the etiological agent of infectious bronchitis, an acute highly contagious economically relevant respiratory disease of poultry. Vaccination is used to control IBV infections, with live-attenuated vaccines generated via serial passage of a virulent field isolate through embryonated hens' eggs. A fine balance must be achieved between attenuation and the retention of immunogenicity. The exact molecular mechanism of attenuation is unknown, and vaccines produced in this manner present a risk of reversion to virulence as few consensus level changes are acquired. Our previous research resulted in the generation of a recombinant IBV (rIBV) known as M41-R, based on a pathogenic strain M41-CK. M41-R was attenuated in vivo by two amino acid changes, Nsp10-Pro85Leu and Nsp14-Val393Leu; however, the mechanism of attenuation was not determined. Pro85 and Val393 were found to be conserved among not only IBV strains but members of the wider coronavirus family. This study demonstrates that the same changes are associated with a temperature-sensitive (ts) replication phenotype at 41°C in vitro, suggesting that the two phenotypes may be linked. Vaccination of specific-pathogen-free chickens with M41-R induced 100% protection against clinical disease, tracheal ciliary damage, and challenge virus replication following homologous challenge with virulent M41-CK. Temperature sensitivity has been used to rationally attenuate other viral pathogens, including influenza, and the identification of amino acid changes that impart both a ts and an attenuated phenotype may therefore offer an avenue for future coronavirus vaccine development. [ABSTRACT FROM AUTHOR] |
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