Inhibitor-resistant TEM- And OXA-1-producing Escherichia coli isolates resistant to amoxicillin-clavulanate are more clonal and possess lower virulence gene content than susceptible clinical isolates
| العنوان: | Inhibitor-resistant TEM- And OXA-1-producing Escherichia coli isolates resistant to amoxicillin-clavulanate are more clonal and possess lower virulence gene content than susceptible clinical isolates |
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| المؤلفون: | Oteo Iglesias, Jesús, González López, Juan José, Ortega Fernández, Adriana, Quintero Zárate, J. Natalia, Bou Arévalo, Germán, Cercenado Mansilla, Emilia, Conejo Gonzalo, María Carmen, Martínez Martínez, Luis, Navarro Risueño, Ferrán, Oliver Palomo, Antonio, Bartolomé Comas, Rosa María, Campos Marqués, José, Spanish Network for Research in Infectious Diseases (REIPI) |
| المساهمون: | Universidad de Sevilla. Departamento de Microbiología, Instituto de Salud Carlos III, Red de Investigación Cooperativa en Investigación en Patología Infecciosa (España), Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), Universidad de Cantabria |
| المصدر: | idUS. Depósito de Investigación de la Universidad de Sevilla instname ANTIMICROBIAL AGENTS AND CHEMOTHERAPY r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau Repisalud Instituto de Salud Carlos III (ISCIII) Antimicrobial Agents and Chemotherapy. 2014 Jul;58(7):3874-81 UCrea Repositorio Abierto de la Universidad de Cantabria Universidad de Cantabria (UC) |
| بيانات النشر: | American Society for Microbiology, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | clone (Java method), musculoskeletal diseases, Virulence Factors, Population, Virulence, Biology, medicine.disease_cause, Amoxicillin-Potassium Clavulanate Combination, beta-Lactamases, Microbiology, Epidemiology and Surveillance, Drug Resistance, Bacterial, medicine, Escherichia coli, Humans, Pharmacology (medical), education, Beta-Lactamase Inhibitors, Escherichia coli Infections, Phylogeny, Pharmacology, Genetics, education.field_of_study, Molecular Epidemiology, Molecular epidemiology, Phylogenetic tree, Anti-Bacterial Agents, Infectious Diseases, Multilocus sequence typing, beta-Lactamase Inhibitors, Multilocus Sequence Typing |
| الوصف: | In a previous prospective multicenter study in Spain, we found that OXA-1 and inhibitor-resistant TEM (IRT) β-lactamases constitute the most common plasmid-borne mechanisms of genuine amoxicillin-clavulanate (AMC) resistance in Escherichia coli . In the present study, we investigated the population structure and virulence traits of clinical AMC-resistant E. coli strains expressing OXA-1 or IRT and compared these traits to those in a control group of clinical AMC-susceptible E. coli isolates. All OXA-1-producing ( n = 67) and IRT-producing ( n = 45) isolates were matched by geographical and temporal origin to the AMC-susceptible control set ( n = 56). We performed multilocus sequence typing and phylogenetic group characterization for each isolate and then studied the isolates for the presence of 49 virulence factors (VFs) by PCR and sequencing. The most prevalent clone detected was distinct for each group: group C isolates of sequence type (ST) 88 (C/ST88) were the most common in OXA-1 producers, B2/ST131 isolates were the most common in IRT producers, and B2/ST73 isolates were the most common in AMC-susceptible isolates. The median numbers of isolates per ST were 3.72 in OXA-1 producers, 2.04 in IRT producers, and 1.69 in AMC-susceptible isolates; the proportions of STs represented by one unique isolate in each group were 19.4%, 31.1%, and 48.2%, respectively. The sum of all VFs detected, calculated as a virulence score, was significantly higher in AMC-susceptible isolates than OXA-1 and IRT producers (means, 12.5 versus 8.3 and 8.2, respectively). Our findings suggest that IRT- and OXA-1-producing E. coli isolates resistant to AMC have a different and less diverse population structure than AMC-susceptible clinical E. coli isolates. The AMC-susceptible population also contains more VFs than AMC-resistant isolates. |
| وصف الملف: | application/pdf |
| تدمد: | 0066-4804 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f302269f4e9ac1a44e0edd5b20cec0fTest https://idus.us.es/handle/11441/65015Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5f302269f4e9ac1a44e0edd5b20cec0f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00664804 |
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