Comparative Microbiome Study of Mummified Peach Fruits by Metagenomics and Metatranscriptomics
| العنوان: | Comparative Microbiome Study of Mummified Peach Fruits by Metagenomics and Metatranscriptomics |
|---|---|
| المؤلفون: | Chang-Gi Back, Won Kyong Cho, Yeonhwa Jo, Hoseong Choi |
| المصدر: | Plants Volume 9 Issue 8 Plants, Vol 9, Iss 1052, p 1052 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Frateuria aurantia, RNA-sequencing, Plant Science, Article, peach, 03 medical and health sciences, mycobiome, Botany, Microbiome, bacteria, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, 0303 health sciences, Monilinia fructigena, DNA shotgun sequencing, Ecology, biology, 030306 microbiology, food and beverages, Monilinia, biology.organism_classification, Metagenomics, QK1-989, Mycovirus, Ewingella americana, fungi, Proteobacteria |
| الوصف: | The dried peach fruits clinging to peach trees or lying on the ground nearby are known as mummified peach fruits. Here, we examined the microbiome communities of three different mummified peach fruits from the nectarine cultivar &ldquo Hahong&rdquo by DNA- and RNA-sequencing. We found the dominance of Monilinia fructigena followed by Sclerotinia borealis, S. sclerotiorum, and Botrytis cinerea in the mummified peach fruits. Moreover, we found a high number of Proteobacteria, including Frateuria aurantia, Neoasaia chiangmaiensis, Robbsia andropogonis, and Ewingella Americana. Furthermore, we identified several viruses and viroids. Bacteriophages were identified by DNA- and RNA-sequencing, while viruses and viroids with RNA genomes were identified by only RNA-sequencing. Moreover, we identified a novel mycovirus referred to as Monilinia umbra-like virus 1 (MULV1) from M. fructigena. Our results revealed the co-inhabitance of fungi and bacteria in the mummified peach fruits, although dominant microorganisms were present. RNA-sequencing revealed that several fungal and bacterial genes were actively transcribed. Comparative analyses suggested that RNA-sequencing provides more detailed information on microbial communities however, combining DNA- and RNA-sequencing results increased the diversity of microorganisms, suggesting the importance of databases and analysis tools for microbiome studies. Taken together, our study provides a comprehensive overview of microbial communities in mummified peach fruits by DNA shotgun sequencing and RNA-sequencing. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2223-7747 |
| DOI: | 10.3390/plants9081052 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38e2fae603ea9f93c82398ed702af389Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....38e2fae603ea9f93c82398ed702af389 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22237747 |
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| DOI: | 10.3390/plants9081052 |