التفاصيل البيبلوغرافية
| العنوان: |
The association of IL-17A rs2275913 single nucleotide polymorphism with anti-tuberculous drug resistance in patients with pulmonary tuberculosis. |
| المؤلفون: |
Elmadbouly, Asmaa A., Abdul-Mohymen, Abeer Mohammed, Eltrawy, Heba H., Abou Elhasan, Hanaa A., Althoqapy, Azza Ali, Amin, Doaa R. |
| المصدر: |
Journal of Genetic Engineering & Biotechnology; 9/4/2023, Vol. 21 Issue 1, p1-9, 9p |
| مصطلحات موضوعية: |
TUBERCULOSIS, SINGLE nucleotide polymorphisms, TUBERCULOSIS patients, DRUG resistance, GENETIC polymorphisms, ENZYME-linked immunosorbent assay |
| مصطلحات جغرافية: |
EGYPT |
| مستخلص: |
Background Drug-resistant Tuberculosis (DR-TB) is a global health burden with high morbidity and mortality in developing countries including Egypt. The susceptibility to infection with DR-TB strains may be genetically determined. Several interleukin gene polymorphisms were investigated as risk factors for tuberculosis infection but focusing on their association with DR-TB was limited. Therefore, the objective of this study is to assess the association of IL 17 - 197 G > A (rs2275913) single nucleotide polymorphism (SNP) with susceptibility to DR-TB strains in comparison to drug-sensitive tuberculosis (DS-TB) strains in Egyptian patients with pulmonary TB. This cross-sectional study was conducted on 80 patients with DR-TB strains and 80 with DS-TB strains as a control group. Both age and sex were comparable among the study's groups. IL-17 - 197 G > A (rs2275913) SNP was genotyped by real-time PCR, and IL-17 serum concentration was measured by enzyme-linked immunosorbent assay (ELISA). Results The GA and AA genotype frequencies of IL 17 - 197 G > A (rs2275913) SNP were significantly higher in patients with DR-TB strains than those with DS-TB strains (p < 0.001). The frequency of the A allele was significantly (p < 0.001) higher in patients with DR-TB group (32.5%) compared to the control group (13.8%). Substantial higher serum levels of IL-17 were detected in the DR-TB group with significant association with AA and AG genotypes. Conclusion Polymorphism in IL-17 -197 G > A (rs2275913) resulted in higher serum levels of IL-17 and Egyptian patients with such polymorphism are three times at risk of infection with DR-TB strains than patients with wild type. [ABSTRACT FROM AUTHOR] |
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