Effect of NIR Laser Therapy by MLS-MiS Source on Fibroblast Activation by Inflammatory Cytokines in Relation to Wound Healing
| العنوان: | Effect of NIR Laser Therapy by MLS-MiS Source on Fibroblast Activation by Inflammatory Cytokines in Relation to Wound Healing |
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| المؤلفون: | Valerio Ciccone, Monica Monici, Elettra Sereni, Shirley Genah, Francesca Cialdai, Lucia Morbidelli |
| المصدر: | Biomedicines, Vol 9, Iss 307, p 307 (2021) Biomedicines Volume 9 Issue 3 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_treatment, Medicine (miscellaneous), Inflammation, wound healing, Article, General Biochemistry, Genetics and Molecular Biology, Proinflammatory cytokine, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Downregulation and upregulation, NIR laser radiation, fibroblasts, photobiomodulation, medicine, Fibroblast, lcsh:QH301-705.5, inflammation, Granulation tissue, Vascular endothelial growth factor, 030104 developmental biology, medicine.anatomical_structure, Cytokine, chemistry, lcsh:Biology (General), 030220 oncology & carcinogenesis, Cancer research, medicine.symptom, Wound healing |
| الوصف: | The fine control of inflammation following injury avoids fibrotic scars or impaired wounds. Due to side effects by anti-inflammatory drugs, the research is continuously active to define alternative therapies. Among them, physical countermeasures such as photobiomodulation therapy (PBMT) are considered effective and safe. To study the cellular and molecular events associated with the anti-inflammatory activity of PBMT by a dual-wavelength NIR laser source, human dermal fibroblasts were exposed to a mix of inflammatory cytokines (IL-1β and TNF-α) followed by laser treatment once a day for three days. Inducible inflammatory key enzymatic pathways, as iNOS and COX-2/mPGES-1/PGE2, were upregulated by the cytokine mix while PBMT reverted their levels and activities. The same behavior was observed with the proangiogenic factor vascular endothelial growth factor (VEGF), involved in neovascularization of granulation tissue. From a molecular point of view, PBMT retained NF-kB cytoplasmatic localization. According to a change in cell morphology, differences in expression and distribution of fundamental cytoskeletal proteins were observed following treatments. Tubulin, F-actin, and α-SMA changed their organization upon cytokine stimulation, while PBMT reestablished the basal localization. Cytoskeletal rearrangements occurring after inflammatory stimuli were correlated with reorganization of membrane α5β1 and fibronectin network as well as with their upregulation, while PBMT induced significant downregulation. Similar changes were observed for collagen I and the gelatinolytic enzyme MMP-1. In conclusion, the present study demonstrates that the proposed NIR laser therapy is effective in controlling fibroblast activation induced by IL-1β and TNF-α, likely responsible for a deleterious effect of persistent inflammation. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2227-9059 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c22ce4cc4e27c619fabebb4e63b5fe72Test https://www.mdpi.com/2227-9059/9/3/307Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c22ce4cc4e27c619fabebb4e63b5fe72 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22279059 |
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