دورية أكاديمية
TLR2 and TLR4 activation induces p38 MAPK-dependent phosphorylation of S6 kinase 1 in C2C12 myotubes.
| العنوان: | TLR2 and TLR4 activation induces p38 MAPK-dependent phosphorylation of S6 kinase 1 in C2C12 myotubes. |
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| المؤلفون: | Zbinden-Foncea, Hermann, Deldicque, Louise, Pierre, Nicolas, Francaux, Marc, Raymackers, Jean-Marc |
| المصدر: | Cell Biology International, 36 (12), 1107-13 (2012) |
| بيانات النشر: | Wiley-Blackwell |
| سنة النشر: | 2012 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Animals, Cell Line, Down-Regulation, Mice, Muscle Fibers, Skeletal/metabolism, Palmitic Acid/metabolism, Phosphorylation, Proto-Oncogene Proteins c-akt/metabolism, Ribosomal Protein S6 Kinases, 90-kDa/metabolism, TOR Serine-Threonine Kinases/metabolism, Toll-Like Receptor 2/genetics/metabolism, Toll-Like Receptor 4/genetics/metabolism, p38 Mitogen-Activated Protein Kinases/metabolism, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | peer reviewed ; Toll-like receptors 2 (TLR2) and 4 (TLR4) are present in the plasma membrane of skeletal muscle cells where their functions remain incompletely resolved. They can bind various extracellular ligands, such as FSL-1, lipopolysaccharide (LPS) and/or palmitic acid (PA). We have investigated the link between PA, TLR2/4 and ribosomal S6 kinase 1 (S6K1) in C2C12 myotubes. Incubation with agonists of either TLR2 or TLR4, and with a high concentration of PA, increased S6K1 phosphorylation. Canonical upstream kinases of S6K1, protein kinase B (PKB) and mammalian target of rapamycin complex 1 (mTORC1), were regulated in the opposite way by PA, indicating that these kinases were probably not involved. By using the SB202190 inhibitor, p38 MAPK (mitogen-activated protein kinase) was found to be a key mediator of PA-induced phosphorylation of S6K1. Downregulation of either tlr2 or tlr4 gene expression by small interfering RNAs prevented the activation of both p38 MAPK and S6K1 by FSL-1, LPS or PA. Thus TLR2 and TLR4 agonists can increase the level of S6K1 phosphorylation in a p38 MAPK-dependent way in C2C12 myotubes. As PA induced the same intracellular signalling, a novel atypical pathway for PA is induced at the cellular membrane level and results in a higher phosphorylation state of S6K1. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1065-6995 1095-8355 |
| العلاقة: | urn:issn:1065-6995; urn:issn:1095-8355; https://orbi.uliege.be/handle/2268/237035Test; info:hdl:2268/237035; scopus-id:2-s2.0-84871998534; info:pmid:22931089 |
| DOI: | 10.1042/CBI20120081 |
| الإتاحة: | https://doi.org/10.1042/CBI20120081Test https://orbi.uliege.be/handle/2268/237035Test |
| حقوق: | restricted access ; http://purl.org/coar/access_right/c_16ecTest ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.6C7592A9 |
| قاعدة البيانات: | BASE |
| تدمد: | 10656995 10958355 |
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| DOI: | 10.1042/CBI20120081 |