دورية أكاديمية
Tri-methylation of H3K79 is decreased in TGF-β1-induced epithelial-to-mesenchymal transition in lung cancer
| العنوان: | Tri-methylation of H3K79 is decreased in TGF-β1-induced epithelial-to-mesenchymal transition in lung cancer |
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| المؤلفون: | Evanno, Emilie, Godet, Julie, Piccirilli, Nathalie, Guilhot, Joëlle, Milin, Serge, Gombert, Jean Marc, Fouchaq, Benoit, Roche, Joëlle |
| المساهمون: | Eurofins-Cerep, Laboratoire de neurosciences expérimentales et cliniques (LNEC Poitiers ), Université de Poitiers = University of Poitiers (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre hospitalier universitaire de Poitiers = Poitiers University Hospital (CHU de Poitiers La Milétrie ), Ischémie Reperfusion en Transplantation d’Organes Mécanismes et Innovations Thérapeutiques U 1082 ( IRTOMIT Poitiers ), INSERM CIC 0802 (INSERM - CHU de Poitiers), Université de Poitiers = University of Poitiers (UP)-Centre hospitalier universitaire de Poitiers = Poitiers University Hospital (CHU de Poitiers La Milétrie )-Institut National de la Santé et de la Recherche Médicale (INSERM), Écologie et biologie des interactions (EBI Poitiers ), Université de Poitiers = University of Poitiers (UP)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 1868-7083 ; Clinical Epigenetics ; https://hal.science/hal-03283985Test ; Clinical Epigenetics, 2017, 9 (1), ⟨10.1186/s13148-017-0380-0⟩. |
| بيانات النشر: | HAL CCSD BioMed Central |
| سنة النشر: | 2017 |
| المجموعة: | Université de Poitiers: Publications de nos chercheurs.ses (HAL) |
| مصطلحات موضوعية: | Lung cancer, NSCLC, EMT, H3K79me3, DOT1L, PD-L1, SEMA3C, NRP2, Epigenetic treatment, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience ; Background : The epithelial-to-mesenchymal transition (EMT) enables epithelial cancer cells to acquire mesenchymal features and contributes to metastasis and resistance to treatment. This process involves epigenetic reprogramming for gene expression. We explored global histone modifications during TGF-β1-induced EMT in two non-small cell lung cancer (NSCLC) cell lines and tested different epigenetic treatment to modulate or partially reverse EMT.Results: Loss of classical epithelial markers and gain of mesenchymal markers were verified in A549 and H358 cell lines during TGF-β1-induced EMT. In addition, we noticed increased expression of the axonal guidance protein semaphorin 3C (SEMA3C) and PD-L1 (programmed death-ligand 1) involved in the inhibition of the immune system, suggesting that both SEMA3C and PD-L1 could be the new markers of TGF-β1-induced EMT. H3K79me3 and H2BK120me1 were decreased in A549 and H358 cell lines after a 48-h TGF-β1 treatment, as well as H2BK120ac in A549 cells. However, decreased H3K79me3 was not associated with expression of the histone methyltransferase DOT1L. Furthermore, H3K79me3 was decreased in tumors compared in normal tissues and not associated with cell proliferation. Associations of histone deacetylase inhibitor (SAHA) with DOT1L inhibitors (EPZ5676 or SGC0946) or BET bromodomain inhibitor (PFI-1) were efficient to partially reverse TGF-β1 effects by decreasing expression of PD-L1, SEMA3C, and its receptor neuropilin-2 (NRP2) and by increasing epithelial markers such as E-cadherin.Conclusion : Histone methylation was modified during EMT, and combination of epigenetic compounds with conventional or targeted chemotherapy might contribute to reduce metastasis and to enhance clinical responses. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/28804523; hal-03283985; https://hal.science/hal-03283985Test; PUBMED: 28804523; PUBMEDCENTRAL: PMC5549304 |
| DOI: | 10.1186/s13148-017-0380-0 |
| الإتاحة: | https://doi.org/10.1186/s13148-017-0380-0Test https://hal.science/hal-03283985Test |
| رقم الانضمام: | edsbas.4F910DA8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13148-017-0380-0 |
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