التفاصيل البيبلوغرافية
| العنوان: |
Effect of TrisEDTA and Chlorhexidine 0.12% on an In Vitro -Defined Biofilm Representing the Subgingival Plaque Biofilm of the Dog. |
| المؤلفون: |
Kling, Katherine E., Maddox, Carol W., Manfra Marretta, Sandra, Nowicki, Christina, Schaeffer, David J. |
| المصدر: |
Journal of Veterinary Dentistry; Mar2022, Vol. 39 Issue 1, p9-20, 12p |
| مصطلحات موضوعية: |
CHLORHEXIDINE, BIOFILMS, TITANIUM alloys, BACTERIAL growth, CANIS, DOGS |
| مستخلص: |
This study was designed to investigate the effects of chlorhexidine 0.12%, TrisEDTA (tromethamine ethylenediamintetraacetic acid), and a combination of chlorhexidine 0.12% and TrisEDTA on an in vitro plaque biofilm model comprised of three bacterial species commonly found in canine subgingival plaque. Porphyromonas gulae, Actinomyces canis, and Neisseria canis were grown in a biofilm on polished hydroxyapatite coated titanium alloy pucks for 72 h prior to exposure to one of four test solutions: TrisEDTA, chlorhexidine 0.12%, a combination of TrisEDTA and chlorhexidine 0.12%, or sterile deionized water as a control. Following exposure to the test solution, a sample was collected of the biofilm either immediately or following 24 h of additional incubation in a broth medium. Lower numbers of CFU/mL of Porphyromonas gulae resulted when the biofilm was treated with a solution of chlorhexidine 0.12% and TrisEDTA compared to with chlorhexidine 0.12% alone, TrisEDTA alone, or the control and so this solution can be said to be synergistic against Porphyromonas gulae in this controlled in vitro model. Greater reductions in the numbers of CFU/mL of Actinomyces canis and Neisseria canis resulted from treatment with chlorhexidine 0.12% alone than if treated with the combination of TrisEDTA and chlorhexidine 0.12%. When treated biofilm samples were allowed 24 h of additional growth in fresh media, greater variance resulted and this variance highlights the complex dynamics involved in bacterial growth within a biofilm. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Veterinary Dentistry is the property of Sage Publications Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
