التفاصيل البيبلوغرافية
| العنوان: |
Assessment of recombinant dog allergens Can f 1 and Can f 2 for the diagnosis of dog allergy. |
| المؤلفون: |
Saarelainen, S., Taivainen, A., Rytkönen-Nissinen, M., Auriola, S., Immonen, A., Mäntyjärvi, R., Rautiainen, J., Kinnunen, T., Virtanen, T. |
| المصدر: |
Clinical & Experimental Allergy; Oct2004, Vol. 34 Issue 10, p1576-1582, 7p |
| مصطلحات موضوعية: |
ALLERGY in dogs, RECOMBINANT molecules, ALLERGY in animals, ALLERGENS, DOG diseases, VETERINARY diagnosis |
| مستخلص: |
The use of recombinant allergens for the diagnosis and immunotherapy of allergy may offer several advantages over allergen extracts.To produce recombinant dog allergens Can f 1 and Can f 2 inPichia pastorisyeast and to assess their suitability for the diagnosis of dog allergy.Clinically diagnosed dog-allergic patients' and healthy non-atopic dog owners' reactivities against recombinant Can f 1 and Can f 2 and commercial dog epithelial extract were studied by a panel of methods including skin prick test (SPT), ELISA and IgE immunoblotting.Recombinant Can f 1 and Can f 2 were found immunologically functional: they bound dog-allergic patients' IgE in immunoblotting and inhibited specifically the binding of IgE to their natural counterparts in the dog allergen extract. Moreover, patients' IgE reactivity in immunoblotting to natural Can f 1 and their SPT with the recombinant allergen were perfectly concordant (φ coefficient 1.0,P<0.001). The concordance was slightly lower with recombinant Can f 2 (φ coefficient 0.92,P<0.001). A lower number of dog-allergic patients, 52%, reacted against Can f 1 than previously reported. About one-third of the patients reacted to Can f 2. In immunoblotting, the highest prevalence of reactivity, 60%, was directed to an 18 kDa component. Aminoterminal sequencing showed this to be a previously unidentified allergenic protein.The recombinant allergens can be used reliably to identify Can f 1 and Can f 2-sensitized individuals. However, the two allergens are insufficient as reagents for diagnosing dog allergy. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
