كتاب
In vitro measurement of DNA base excision repair in isolated mitochondria.
| العنوان: | In vitro measurement of DNA base excision repair in isolated mitochondria. |
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| المؤلفون: | Page, Melissa, Stuart JA |
| المساهمون: | Brock University, Canada - Departement of Biological Sciences |
| المصدر: | Methods in Molecular Biology, Vol. 554, p. 213-231 |
| بيانات النشر: | Humana Press, Inc. |
| سنة النشر: | 2009 |
| المجموعة: | DIAL@USL-B (Université Saint-Louis, Bruxelles) |
| مصطلحات موضوعية: | mitochondria, mtDNA, base excision repair (BER), glycosylase, AP endonuclease, saccharomyces cerevisiae, DNA repair |
| الوصف: | Mitochondrial DNA (mtDNA) is in relatively close proximity to reactive oxygen species (ROS) arising from spontaneous superoxide formation during respiration. As a result, it sustains oxidative damage that may include base modifications, base loss, and strand breaks. mtDNA replication past sites of oxidative damage can result in the introduction of mutations. mtDNA mutations are associated with various human diseases and can manifest as loss of bioenergetic function. DNA repair processes exist in mitochondria from apparently all metazoans. A fully functional DNA base excision repair (BER) pathway is present in mitochondria of vertebrates. This pathway is catalyzed by a number of DNA glycosylases, an AP endonuclease, polymerase gamma, and a DNA ligase. This chapter outlines the step-by-step protocols for isolating mitochondrial fractions, from a number of different model organisms, of sufficient purity to allow mtDNA repair activities to be measured. It details in vitro assays for the measurement of BER enzyme activities in lysates prepared from isolated mitochondria. |
| نوع الوثيقة: | book part |
| اللغة: | English |
| تدمد: | 1064-3745 1940-6029 |
| العلاقة: | boreal:193867; http://hdl.handle.net/2078/193867Test; urn:ISSN:1064-3745; urn:EISSN:1940-6029 |
| DOI: | 10.1007/978-1-59745-521-3_14 |
| الإتاحة: | https://doi.org/10.1007/978-1-59745-521-3_14Test http://hdl.handle.net/2078/193867Test |
| رقم الانضمام: | edsbas.1020B6B2 |
| قاعدة البيانات: | BASE |
| تدمد: | 10643745 19406029 |
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| DOI: | 10.1007/978-1-59745-521-3_14 |