المؤلفون: Agarwal, Ashok, Farkouh, Ala'a, Saleh, Ramadan, Hamoda, Taha Abo-Almagd Abdel-Meguid, Salvio, Gianmaria, Boitrelle, Florence, Harraz, Ahmed M, Ghayda, Ramy Abou, Kavoussi, Parviz, Gül, Murat, Toprak, Tuncay, Russo, Giorgio Ivan, Durairajanayagam, Damayanthi, Rambhatla, Amarnath, Birowo, Ponco, Cannarella, Rossella, Phuoc, Nguyen Ho Vinh, Zini, Armand, Arafa, Mohamed, Wyns, Christine, Tremellen, Kelton, Sarıkaya, Selçuk, Lewis, Sheena, Evenson, Donald P, Ko, Edmund, Calogero, Aldo E, Bahar, Fahmi, Martínez, Marlon, Ambar, Rafael F, Colpi, Giovanni M, Bakircioglu, Mustafa Emre, Henkel, Ralf, Kandil, Hussein, Serefoglu, Ege Can, Alfakhri, Abdullah, Tsujimura, Akira, Kheradmand, Alireza, Marino, Angelo, Adamyan, Aram, Zilaitiene, Birute, Ozer, Cevahir, Pescatori, Edoardo, Vogiatzi, Paraskevi, Busetto, Gian Maria, Balercia, Giancarlo, Elbardisi, Haitham, Akhavizadegan, Hamed, Sajadi, Hesamoddin, Taniguchi, Hisanori, Park, Hyun Jun, Maldonado Rosas, Israel, Al-Marhoon, Mohamed, Sadighi Gilani, Mohammad Ali, Alhathal, Naif, Quang, Nguyen, Pinggera, Germar-Michael, Kothari, Priyank, Micic, Sava, Homa, Sheryl, Long, Tran Quang Tien, Zohdy, Wael, Atmoko, Widi, Ibrahim, Wael, Sabbaghian, Marjan, Abumelha, Saad Mohammed, Chung, Eric, Ugur, Muhammet Rasit, Ozkent, Mehmet Serkan, Selim, Osama, Darbandi, Mahsa, Fukuhara, Shinichiro, Jamali, Mounir, de la Rosette, Jean, Kuroda, Shinnosuke, Smith, Ryan P, Baser, Aykut, Kalkanli, Arif, Tadros, Nicholas N, Aydos, Kaan, Mierzwa, Tiago Cesar, Khalafalla, Kareim, Malhotra, Vineet, Moussa, Mohamad, Finocchi, Federica, Rachman, Rinaldo Indra, Giulioni, Carlo, Avidor-Reiss, Tomer, Kahraman, Oguzhan, Çeker, Gökhan, Zenoaga-Barbăroșie, Cătălina, Barrett, Trenton L, Yilmaz, Mehmet, Kadioglu, Ates, Jindal, Sunil, Omran, Huda, Bocu, Kadir, Karthikeyan, Vilvapathy Senguttuvan, Franco, Giorgio, Solorzano, Jesús Fernando, Vishwakarma, Ranjit B, Arianto, Eko, Garrido, Nicolas, Jain, Divyanu, Gherabi, Nazim, Sokolakis, Ioannis, Palani, Ayad, Calik, Gokhan, Kulaksiz, Deniz, Simanaviciene, Vaida, Simopoulou, Mara, Güngör, Nur Dokuzeylül, Blecher, Gideon, Falcone, Marco, Jezek, Davor, Preto, Mirko, Amar, Edouard, Le, Tan V, Ahn, Sun Tae, Rezano, Andri, Singh, Keerti, Rocco, Lucia, Savira, Missy, Rajmil, Osvaldo, Darbandi, Sara, Sogutdelen, Emrullah, Boeri, Luca, Hernández, Guadalupe, Hakim, Lukman, Morimoto, Yoshiharu, Japari, Andrian, Sofikitis, Nikolaos, Altay, Baris, Metin Mahmutoglu, Asli, Al Hashimi, Manaf, Ziouziou, Imad, Anagnostopoulou, Christina, Lin, Haocheng, Shah, Rupin

المساهمون: Agarwal, Ashok, Farkouh, Ala'A, Saleh, Ramadan, Hamoda, Taha Abo-Almagd Abdel-Meguid, Salvio, Gianmaria, Boitrelle, Florence, Harraz, Ahmed M, Ghayda, Ramy Abou, Kavoussi, Parviz, Gül, Murat, Toprak, Tuncay, Russo, Giorgio Ivan, Durairajanayagam, Damayanthi, Rambhatla, Amarnath, Birowo, Ponco, Cannarella, Rossella, Phuoc, Nguyen Ho Vinh, Zini, Armand, Arafa, Mohamed, Wyns, Christine, Tremellen, Kelton, Sarıkaya, Selçuk, Lewis, Sheena, Evenson, Donald P, Ko, Edmund, Calogero, Aldo E, Bahar, Fahmi, Martínez, Marlon, Ambar, Rafael F, Colpi, Giovanni M, Bakircioglu, Mustafa Emre, Henkel, Ralf, Kandil, Hussein, Serefoglu, Ege Can, Alfakhri, Abdullah, Tsujimura, Akira, Kheradmand, Alireza, Marino, Angelo, Adamyan, Aram, Zilaitiene, Birute, Ozer, Cevahir, Pescatori, Edoardo, Vogiatzi, Paraskevi, Busetto, Gian Maria, Balercia, Giancarlo, Elbardisi, Haitham, Akhavizadegan, Hamed, Sajadi, Hesamoddin, Taniguchi, Hisanori, Park, Hyun Jun, Maldonado Rosas, Israel, Al-Marhoon, Mohamed, Sadighi Gilani, Mohammad Ali, Alhathal, Naif, Quang, Nguyen, Pinggera, Germar-Michael, Kothari, Priyank, Micic, Sava, Homa, Sheryl, Long, Tran Quang Tien, Zohdy, Wael, Atmoko, Widi, Ibrahim, Wael, Sabbaghian, Marjan, Abumelha, Saad Mohammed, Chung, Eric, Ugur, Muhammet Rasit, Ozkent, Mehmet Serkan, Selim, Osama, Darbandi, Mahsa, Fukuhara, Shinichiro, Jamali, Mounir, de la Rosette, Jean, Kuroda, Shinnosuke, Smith, Ryan P, Baser, Aykut, Kalkanli, Arif, Tadros, Nicholas N, Aydos, Kaan, Mierzwa, Tiago Cesar, Khalafalla, Kareim, Malhotra, Vineet, Moussa, Mohamad, Finocchi, Federica, Rachman, Rinaldo Indra, Giulioni, Carlo, Avidor-Reiss, Tomer, Kahraman, Oguzhan, Çeker, Gökhan, Zenoaga-Barbăroșie, Cătălina, Barrett, Trenton L, Yilmaz, Mehmet, Kadioglu, Ate, Jindal, Sunil, Omran, Huda, Bocu, Kadir, Karthikeyan, Vilvapathy Senguttuvan, Franco, Giorgio, Solorzano, Jesús Fernando, Vishwakarma, Ranjit B

مصطلحات موضوعية: DNA fragmentation, Delphi method, Diagnostic test, Infertility, male, Survey

الوصف: Purpose: Sperm DNA fragmentation (SDF) is a functional sperm abnormality that can impact reproductive potential, for which four assays have been described in the recently published sixth edition of the WHO laboratory manual for the exami-nation and processing of human semen. The purpose of this study was to examine the global practices related to the use of SDF assays and investigate the barriers and limitations that clinicians face in incorporating these tests into their practice. Materials and Methods: Clinicians managing male infertility were invited to complete an online survey on practices related to SDF diagnostic and treatment approaches. Their responses related to the technical aspects of SDF testing, current profes-sional society guidelines, and the literature were used to generate expert recommendations via the Delphi method. Finally, challenges related to SDF that the clinicians encounter in their daily practice were captured. Results: The survey was completed by 436 reproductive clinicians. Overall, terminal deoxynucleotidyl transferase deoxyuri-dine triphosphate Nick-End Labeling (TUNEL) is the most commonly used assay chosen by 28.6%, followed by the sperm chromatin structure assay (24.1%), and the sperm chromatin dispersion (19.1%). The choice of the assay was largely in-fluenced by availability (70% of respondents). A threshold of 30% was the most selected cut-off value for elevated SDF by 33.7% of clinicians. Of respondents, 53.6% recommend SDF testing after 3 to 5 days of abstinence. Although 75.3% believe SDF testing can provide an explanation for many unknown causes of infertility, the main limiting factors selected by respon-dents are a lack of professional society guideline recommendations (62.7%) and an absence of globally accepted references for SDF interpretation (50.3%). Conclusions: This study represents the largest global survey on the technical aspects of SDF testing as well as the barriers en-countered by clinicians. Unified global recommendations regarding clinician implementation ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37635341; info:eu-repo/semantics/altIdentifier/wos/WOS:001063965600001; volume:42; issue:1; firstpage:202; lastpage:215; numberofpages:14; journal:THE WORLD JOURNAL OF MEN'S HEALTH; https://hdl.handle.net/11566/325985Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85173813215

الإتاحة: https://doi.org/10.5534/wjmh.230076Test
https://hdl.handle.net/11566/325985Test

المؤلفون: Agarwal, Ashok, Farkouh, Ala’a, Saleh, Ramadan, Abdel-Meguid Hamoda, Taha Abo-Almagd, Harraz, Ahmed, M, Kavoussi, Parviz, Arafa, Mohamed, Salvio, Gianmaria, Rambhatla, Amarnath, Toprak, Tuncay, Gül, Murat, Phuoc, Nguyen, Ho Vinh, Boitrelle, Florence, Birowo, Ponco, Ghayda, Ramy, Abou, Cannarella, Rossella, Kuroda, Shinnosuke, Durairajanayagam, Damayanthi, Zini, Armand, Wyns, Christine, Sarikaya, Selcuk, Tremellen, Kelton, Mostafa, Taymour, Sokolakis, Ioannis, Evenson, Donald, P, Henkel, Ralf, Zohdy, Wael, Chung, Eric, Ziouziou, Imad, Falcone, Marco, Russo, Giorgio, I, Al-Hashimi, Manaf, Calogero, Aldo, E, Ko, Edmund, Colpi, Giovanni, Lewis, Sheena, Serefoglu, Ege, Can, Bahar, Fahmi, Martinez, Marlon, Nguyen, Quang, Ambar, Rafael, F, Bakircioglu, Mustafa, Emre, Kandil, Hussein, Mogharabian, Nasser, Sabbaghian, Marjan, Taniguchi, Hisanori, Tsujimura, Akira, Sajadi, Hesamoddin, Ibrahim, Wael, Atmoko, Widi, Vogiatzi, Paraskevi, Gunes, Sezgin, Gilani, Mohammad, Ali Sadighi, Roychoudhury, Shubhadeep, Güngör, Nur, Dokuzeylül, Hakim, Lukman, Adriansjah, Ricky, Kothari, Priyank, Jindal, Sunil, Amar, Edouard, Park, Hyun, Jun, Long, Tran, Quang Tien, Homa, Sheryl, Zilaitiene, Birute, Rosas, Israel, Maldonado, Marino, Angelo, Pescatori, Edoardo, Ozer, Cevahir, Akhavizadegan, Hamed, Garrido, Nicolas, Busetto, Gian, Maria, Adamyan, Aram, Al-Marhoon, Mohamed, Elbardisi, Haitham, Dolati, Parisa, Darbandi, Mahsa, Darbandi, Sara, Balercia, Giancarlo, Pinggera, Germar-Michael, Micic, Sava, Ho, Christopher, Chee Kong, Moussa, Mohamad, Preto, Mirko, Zenoaga-Barbăroşie, Cătălina, Smith, Ryan, P, Kosgi, Raghavender, Rosette, Jean, de La, El-Sakka, Ahmed, I, Abumelha, Saad, Mohammed, Mierzwa, Tiago, Cesar, Ong, Teng, Aik, Banihani, Saleem, A, Bowa, Kasonde, Fukuhara, Shinichiro, Boeri, Luca, Danacıoğlu, Yavuz, Onur, Gokalp, Fatih, Selim, Osama, Mohamed, Cho, Chak-Lam, Tadros, Nicholas, N, Ugur, Muhammet, Rasit, Ozkent, Mehmet, Serkan, Chiu, Peter, Kalkanli, Arif, Khalafalla, Kareim, Vishwakarma, Ranjit, B, Finocchi, Federica, Andreadakis, Sotiris, Giulioni, Carlo, Çeker, Gökhan, Ceyhan, Erman, Malhotra, Vineet, Yilmaz, Mehmet, Timpano, Massimiliano, Barrett, Trenton, L, Kim, Shannon, Hee Kyung, Ahn, Sun-Tae, Giacone, Filippo, Palani, Ayad, Duarsa, Gede, Wirya Kusuma, Kadioglu, Ates, Gadda, Franco, Zylbersztejn, Daniel, Suslik, Aydos, Kaan, Kulaksız, Deniz, Gupte, Deepak, Calik, Gokhan, Karna, Keshab, Kumar, Drakopoulos, Panagiotis, Baser, Aykut, Kumar, Vijay, Molina, Juan, Manuel Corral, Rajmil, Osvaldo, Ferreira, Raphael, H, Leonardi, Sofia, Avoyan, Armen, Sogutdelen, Emrullah, Franco, Giorgio, Ramsay, Jonathan, Ramirez, Liliana, Shah, Rupin, Karthikeyan, Vilvapathy Senguttuvan

المساهمون: Cleveland Clinic, Sohag University, King Abdulaziz University, Minia University, Mansoura University Egypt, Weill Cornell Medical College-Qatar, Qatar, Cairo University, Polytechnic University of Marche Ancona, Italy / Università Politecnica delle Marche Ancona, Italia (UNIVPM), Vattikuti Urology Institute, Henry Ford Hospital, Faculty of Medicine Selcuk University Konya, Turkey, Selçuk University = Selçuk Üniversitesi, CHI Poissy-Saint-Germain, Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Cipto Mangunkusumo Hospital Jakarta, Case Western Reserve University, Cleveland, Università degli studi di Catania = University of Catania (Unict), Universiti Teknologi MARA Shah Alam (UiTM ), McGill University = Université McGill Montréal, Canada, Cliniques Universitaires Saint-Luc Bruxelles, Gulhane Training and Research Hospital, Flinders University of South Australia, Department of Urology, Martha-Maria Hospital Nuremberg, Nuremberg, Germany., Imperial College London, University of the Western Cape (UWC), Princess Alexandra Hospital, Brisbane, Molinette Hospital, Burjeel Hospital, Khalifa University, Loma Linda University, Biruni University, University of Santo Tomas Manila, Philippines, Vietnam National University Hanoï (VNU), Shahroud University of Medical Sciences, Royan Institute for Reproductive Biomedicine Tehran, Iran, Kansai University, Ondokuz Mayis University (OMU), Assam University, Bahcesehir University Istanbul, Universitas Airlangga (UNAIR), Universitas Padjadjaran (UnPad), Hôpital Américain de Paris, Pusan National University (PNU), University of Kent Canterbury, Lithuanian University of health Sciences (LSMU), Başkent University Hospital Adana, Turkey, Tehran University of Medical Sciences (TUMS), Università degli Studi di Foggia = University of Foggia (Unifg), Sultan Qaboos University (SQU), Hamad Medical Corporation Doha, Qatar, Weill Cornell Medicine Qatar, Shiraz University (Shiraz University ), Innsbruck Medical University = Medizinische Universität Innsbruck (IMU), Taylor’s University, الجامعة اللبنانية بيروت = Lebanese University Beirut = Université libanaise Beyrouth (LU / ULB), University of Bucharest (UniBuc), University of Virginia, Istanbul Medipol University, University of Malaya = Universiti Malaya Kuala Lumpur, Malaisie (UM), Jordan University of Science and Technology Irbid, Jordan (JUST), University of Zambia Lusaka (UNZA), Osaka University Osaka, Hatay Mustafa Kemal University, Cairo University - Faculty of Medicine, The Chinese University of Hong Kong Hong Kong (CUHK), University of Illinois Chicago (UIC), University of Illinois System, Lilavati Hospital and Research Centre, Universitäts Klinikum Freiburg = University Medical Center Freiburg (Uniklinik), Macquarie University Sydney, University of Garmian, Udayana University Bali, Istanbul University, Ankara Üniversitesi, Universitair Ziekenhuis Brussel = University Hospital of Brussels (UZ Brussel), Kurukshetra University, Clinic Barcelona Hospital Universitari, Fundació Puigvert Barcelona, Spain, Abant Izzet Baysal University - Bolu Abant İzzet Baysal Üniversitesi, Università degli Studi di Roma "La Sapienza" = Sapienza University Rome (UNIROMA), Hammersmith Hospital NHS Imperial College Healthcare

المصدر: ISSN: 2287-4208.

مصطلحات موضوعية: Delphi method, DNA fragmentation, Male infertility, Practice guidelines, Sperm, Survey, [SDV]Life Sciences [q-bio]

الوصف: All authors are members of the Global Andrology Forum ; International audience ; Sperm DNA fragmentation (SDF) testing was recently added to the sixth edition of the World Health Organization laboratory manual for the examination and processing of human semen. Many conditions and risk factors have been associated with elevated SDF ; therefore, it is important to identify the population of infertile men who might benefit from this test.The purpose of this study was to investigate global practices related to indications for SDF testing, compare the relevant professional society guideline recommendations, and provide expert recommendations.Clinicians managing male infertility were invited to take part in a global online survey on SDF clinical practices. This was conducted following the CHERRIES checklist criteria. The responses were compared to professional society guideline recommendations related to SDF and the appropriate available evidence. Expert recommendations on indications for SDF testing were then formulated, and the Delphi method was used to reach consensus.The survey was completed by 436 experts from 55 countries. Almost 75% of respondents test for SDF in all or some men with unexplained or idiopathic infertility, 39% order it routinely in the work-up of recurrent pregnancy loss (RPL), and 62.2% investigate SDF in smokers. While 47% of reproductive urologists test SDF to support the decision for varicocele repair surgery when conventional semen parameters are normal, significantly fewer general urologists (23%; p=0.008) do the same.Nearly 70% would assess SDF before assisted reproductive technologies (ART), either always or for certain conditions. Recurrent ART failure is a common indication for SDF testing. Very few society recommendations were found regarding SDF testing.This article presents the largest global survey on the indications for SDF testing in infertile men, and demonstrates diverse practices. Furthermore, it highlights the paucity of professional society guideline recommendations. Expert ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37118960; hal-04122488; https://hal.inrae.fr/hal-04122488Test; https://hal.inrae.fr/hal-04122488/documentTest; https://hal.inrae.fr/hal-04122488/file/2074WJMH_wjmh-41-e43.pdfTest; PUBMED: 37118960; WOS: 001081903000008

الإتاحة: https://doi.org/10.5534/wjmh.220282Test
https://hal.inrae.fr/hal-04122488Test
https://hal.inrae.fr/hal-04122488/documentTest
https://hal.inrae.fr/hal-04122488/file/2074WJMH_wjmh-41-e43.pdfTest

المؤلفون: Farkouh, Ala’a, Agarwal, Ashok, Hamoda, Taha Abo-Almagd Abdel-Meguid, Kavoussi, Parviz, Saleh, Ramadan, Zini, Armand, Arafa, Mohamed, Harraz, Ahmed, M, Gul, Murat, Karthikeyan, Vilvapathy, Senguttuvan, Durairajanayagam, Damayanthi, Rambhatla, Amarnath, Boitrelle, Florence, Chung, Eric, Birowo, Ponco, Toprak, Tuncay, Ghayda, Ramy, Abou, Cannarella, Rossella, Phuoc, Nguyen, Ho Vinh, Dimitriadis, Fotios, Russo, Giorgio, I, Sokolakis, Ioannis, Mostafa, Taymour, Makarounis, Konstantinos, Ziouziou, Imad, Kuroda, Shinnosuke, Bendayan, Marion, Kaiyal, Raneen, Sawaid, Japari, Andrian, Simopoulou, Mara, Rocco, Lucia, Garrido, Nicolas, Gherabi, Nazim, Bocu, Kadir, Kahraman, Oguzhan, Le, Tan, V, Wyns, Christine, Tremellen, Kelton, Sarikaya, Selcuk, Lewis, Sheena, Evenson, Donald, P, Ko, Edmund, Calogero, Aldo, E, Bahar, Fahmi, Martinez, Marlon, Crafa, Andrea, Nguyen, Quang, Ambar, Rafael, F, Colpi, Giovanni, Bakircioglu, Mustafa, Emre, Henkel, Ralf, Kandil, Hussein, Serefoglu, Ege, Can, Alarbid, Abdullah, Tsujimura, Akira, Kheradmand, Alireza, Anagnostopoulou, Christina, Marino, Angelo, Adamyan, Aram, Zilaitiene, Birute, Ozer, Cevahir, Pescatori, Edoardo, Vogiatzi, Paraskevi, Busetto, Gian, Maria, Balercia, Giancarlo, Elbardisi, Haitham, Akhavizadegan, Hamed, Sajadi, Hesamoddin, Taniguchi, Hisanori, Park, Hyun, Jun, Rosas, Israel, Maldonado, Al-Marhoon, Mohamed, Gilani, Mohammad, Ali Sadighi, Alhathal, Naif, Pinggera, Germar-Michael, Kothari, Priyank, Mogharabian, Nasser, Micic, Sava, Homa, Sheryl, Darbandi, Sara, Long, Tran, Quang Tien, Zohdy, Wael, Atmoko, Widi, Sabbaghian, Marjan, Ibrahim, Wael, Smith, Ryan, P, Ho, Christopher, Chee Kong, Rosette, Jean, de La, El-Sakka, Ahmed, I, Preto, Mirko, Zenoaga-Barbăroșie, Cătălina, Abumelha, Saad, Mohammed, Baser, Aykut, Aydos, Kaan, Ramirez-Dominguez, Liliana, Kumar, Vijay, Ong, Teng, Aik, Mierzwa, Tiago, Cesar, Adriansjah, Ricky, Banihani, Saleem, A, Bowa, Kasonde, Fukuhara, Shinichiro, Peña, Marcelo, Rodriguez, Moussa, Mohamad, Ari, Umut, Çağın, Cho, Chak-Lam, Tadros, Nicholas, N, Ugur, Muhammet, Rasit, Amar, Edouard, Falcone, Marco, Santer, Frederic, Romain, Kalkanli, Arif, Karna, Keshab, Kumar, Khalafalla, Kareim, Vishwakarma, Ranjit, B, Finocchi, Federica, Giulioni, Carlo, Ceyhan, Erman, Çeker, Gökhan, Yazbeck, Chadi, Rajmil, Osvaldo, Yilmaz, Mehmet, Altay, Baris, Barrett, Trenton, L, Ngoo, Kay, Seong, Roychoudhury, Shubhadeep, Salvio, Gianmaria, Lin, Haocheng, Kadioglu, Ates, Timpano, Massimiliano, Avidor-Reiss, Tomer, Hakim, Lukman, Sindhwani, Puneet, Franco, Giorgio, Singh, Rajender, Giacone, Filippo, Ruzaev, Mikhail, Kosgi, Raghavender, Sofikitis, Nikolaos, Palani, Ayad, Calik, Gokhan, Kulaksız, Deniz, Jezek, Davor, Al Hashmi, Manaf, Drakopoulos, Panagiotis, Omran, Huda, Leonardi, Sofia, Celik-Ozenci, Ciler, Güngör, Nur, Dokuzeylül, Ramsay, Jonathan, Amano, Toshiyasu, Sogutdelen, Emrullah, Duarsa, Gede, Wirya Kusuma, Chiba, Koji, Jindal, Sunil, Savira, Missy, Boeri, Luca, Borges, Edson, Gupte, Deepak, Gokalp, Fatih, Hebrard, Guadalupe, Hernández, Minhas, Suks, Shah, Rupin

المساهمون: Cleveland Clinic, King Abdulaziz University, Minia University, Sohag University, McGill University = Université McGill Montréal, Canada, Hamad Medical Corporation Doha, Qatar, Cairo University, Weill Cornell Medicine Qatar, Mansoura University Egypt, Faculty of Medicine Selcuk University Konya, Turkey, Selçuk University = Selçuk Üniversitesi, Apollo Hospital, Bilaspur(C. G.), India, Universiti Teknologi MARA Shah Alam (UiTM ), Vattikuti Urology Institute, Henry Ford Hospital, CHI Poissy-Saint-Germain, Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Princess Alexandra Hospital, Brisbane, Cipto Mangunkusumo Hospital Jakarta, Case Western Reserve University Cleveland, Università degli studi di Catania = University of Catania (Unict), Aristotle University of Thessaloniki, Université Ibn Zohr Agadir, Centre d’Études et de Conservation des Œufs et du Sperme CHU Jean Verdier (CECOS), Hôpital Jean Verdier AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), National and Kapodistrian University of Athens (NKUA), Università degli studi della Campania "Luigi Vanvitelli" = University of the Study of Campania Luigi Vanvitelli, University of Algiers 3 : Université d' Alger 3, Başkent University Hospital Adana, Turkey, Université Catholique de Louvain = Catholic University of Louvain (UCL), Flinders University of South Australia, Gulhane Training and Research Hospital, Loma Linda University, University of Santo Tomas Manila, Philippines, Vietnam National University Hanoï (VNU), Imperial College London, University of the Western Cape (UWC), Biruni University Istanbul (BU), Ahvaz Jundishapur University of Medical Sciences (AJUMS), Lithuanian University of health Sciences (LSMU), Baskent University, Università degli Studi di Foggia = University of Foggia (Unifg), Polytechnic University of Marche Ancona, Italy / Università Politecnica delle Marche Ancona, Italia (UNIVPM), Hamad medical corporation, Tehran University of Medical Sciences (TUMS), Royan Institute for Reproductive Biomedicine Tehran, Iran, Kansai Medical University, Pusan National University (PNU), Sultan Qaboos University (SQU), King Faisal Specialist Hospital and Research Centre (KFSH & RC), Innsbruck Medical University = Medizinische Universität Innsbruck (IMU), Dept. of Microbiology, Topiwala National Medical College and B. Y. L. Nair Charitable Hospital, Mumbai: 400008, India, Shahroud University of Medical Sciences, University of Kent Canterbury, Hope Generation Foundation, Taylor’s University, Istanbul Medipol University, Suez Canal University, Università degli studi di Torino = University of Turin (UNITO), University of Bucharest (UniBuc), King Abdulaziz Medical City, Department of Surgery, Riyadh, Saudi Arabia, Ankara University School of Medicine Turkey, Kurukshetra University, University of Malaya = Universiti Malaya Kuala Lumpur, Malaisie (UM), Universitas Padjadjaran (UnPad), Jordan University of Science and Technology Irbid, Jordan (JUST), University of Zambia Lusaka (UNZA), Osaka University Osaka, الجامعة اللبنانية بيروت = Lebanese University Beirut = Université libanaise Beyrouth (LU / ULB), Al-Zahraa Hospital University Medical Center (ZHUMC), Kafkas University, The Chinese University of Hong Kong Hong Kong (CUHK), University of Illinois Springfield, Hôpital Américain de Paris, University of Massachusetts Medical School Worcester (UMASS), University of Massachusetts System (UMASS), McGovern Medical School Houston, Texas, The University of Texas Health Science Center at Houston (UTHealth), MD Anderson Cancer Center Houston, Lilavati Hospital and Research Centre, Institut médical Reprogynes, Fundació Puigvert Barcelona, Spain, Ege University Izmir, Assam University, Peking University Beijing, Istanbul University, University of Toledo, Universitas Airlangga (UNAIR), Università degli Studi di Roma "La Sapienza" = Sapienza University Rome (UNIROMA), CSIR-Central Drug Research Institute (CSIR-CDRI), Council of Scientific and Industrial Research India (CSIR), University of Ioannina, University of Garmian, University Hospital Centre Zagreb, Partenaires INRAE, Burjeel Hospital

المصدر: ISSN: 2287-4208.

مصطلحات موضوعية: Delphi method, Disease management, DNA fragmentation, Male infertility, Practice guideline, Survey, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology

الوصف: International audience ; Sperm DNA fragmentation (SDF) has been associated with male infertility and poor outcomes of assisted reproduc-tive technology (ART). The purpose of this study was to investigate global practices related to the management of elevated SDF in infertile men, summarize the relevant professional society recommendations, and provide expert recommendations for managing this condition. An online global survey on clinical practices related to SDF was disseminated to reproductive clini-cians, according to the CHERRIES checklist criteria. Management protocols for various conditions associated with SDF were captured and compared to the relevant recommendations in professional society guidelines and the appropriate available evidence. Expert recommendations and consensus on the management of infertile men with elevated SDF were then formu-lated and adapted using the Delphi method. A total of 436 experts from 55 different countries submitted responses. As an initial approach, 79.1% of reproductive experts recommend lifestyle modifications for infertile men with elevated SDF, and 76.9% prescribe empiric antioxidants. Regarding antioxidant duration, 39.3% recommend 4-6 months and 38.1% recommend 3 months. For men with unex-plained or idiopathic infertility, and couples experiencing recurrent miscarriages associated with elevated SDF, most respon-dents refer to ART 6 months after failure of conservative and empiric medical management. Infertile men with clinical vari-cocele, normal conventional semen parameters, and elevated SDF are offered varicocele repair immediately after diagnosis by 31.4%, and after failure of antioxidants and conservative measures by 40.9%. Sperm selection techniques and testicular sperm extraction are also management options for couples undergoing ART. For most questions, heterogenous practices were demonstrated.This paper presents the results of a large global survey on the management of infertile men with elevated SDF and reveals a lack of consensus among clinicians. ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37118965; hal-04161008; https://hal.inrae.fr/hal-04161008Test; https://hal.inrae.fr/hal-04161008/documentTest; https://hal.inrae.fr/hal-04161008/file/2074WJMH_wjmh-41-e48.pdfTest; PUBMED: 37118965; WOS: 000992530900001

الإتاحة: https://doi.org/10.5534/wjmh.230008Test
https://hal.inrae.fr/hal-04161008Test
https://hal.inrae.fr/hal-04161008/documentTest
https://hal.inrae.fr/hal-04161008/file/2074WJMH_wjmh-41-e48.pdfTest

المؤلفون: Agarwal, Ashok, Farkouh, Ala’a, Saleh, Ramadan, Hamoda, Taha Abo-Almagd Abdel-Meguid, Salvio, Gianmaria, Boitrelle, Florence, Harraz, Ahmed, Abou Ghayda, Ramy, Kavoussi, Parviz, Gül, Murat, Toprak, Tuncay, Russo, Giorgio Ivan, Durairajanayagam, Damayanthi, Rambhatla, Amarnath, Birowo, Ponco, Cannarella, Rossella, Phuoc, Nguyen Ho Vinh, Zini, Armand, Arafa, Mohamed, Wyns, Christine, Tremellen, Kelton, Sarıkaya, Selçuk, Lewis, Sheena, Evenson, Donald, Ko, Edmund, Calogero, Aldo, Bahar, Fahmi, Martínez, Marlon, Ambar, Rafael, Colpi, Giovanni, Bakircioglu, Mustafa Emre, Henkel, Ralf, Kandil, Hussein, Serefoglu, Ege Can, Alfakhri, Abdullah, Tsujimura, Akira, Kheradmand, Alireza, Marino, Angelo, Adamyan, Aram, Zilaitiene, Birute, Ozer, Cevahir, Pescatori, Edoardo, Vogiatzi, Paraskevi, Busetto, Gian Maria, Balercia, Giancarlo, Elbardisi, Haitham, Akhavizadegan, Hamed, Sajadi, Hesamoddin, Taniguchi, Hisanori, Park, Hyun Jun, Maldonado Rosas, Israel, Al-Marhoon, Mohamed, Sadighi Gilani, Mohammad Ali, Alhathal, Naif, Quang, Nguyen, Pinggera, Germar-Michael, Kothari, Priyank, Micic, Sava, Homa, Sheryl, Long, Tran Quang Tien, Zohdy, Wael, Atmoko, Widi, Ibrahim, Wael, Sabbaghian, Marjan, Abumelha, Saad Mohammed, Chung, Eric, Ugur, Muhammet Rasit, Ozkent, Mehmet Serkan, Selim, Osama, Darbandi, Mahsa, Fukuhara, Shinichiro, Jamali, Mounir, de la Rosette, Jean, Kuroda, Shinnosuke, Smith, Ryan, Baser, Aykut, Kalkanli, Arif, Tadros, Nicholas, Aydos, Kaan, Mierzwa, Tiago Cesar, Khalafalla, Kareim, Malhotra, Vineet, Moussa, Mohamad, Finocchi, Federica, Rachman, Rinaldo Indra, Giulioni, Carlo, Avidor-Reiss, Tomer, Kahraman, Oguzhan, Çeker, Gökhan, Zenoaga-Barbăroşie, Cătălina, Barrett, Trenton, Yilmaz, Mehmet, Kadioglu, Ates, Jindal, Sunil, Omran, Huda, Bocu, Kadir, Karthikeyan, Vilvapathy Senguttuvan, Franco, Giorgio, Solorzano, Jesús Fernando, Vishwakarma, Ranjit, Arianto, Eko, Garrido, Nicolas, Jain, Divyanu, Gherabi, Nazim, Sokolakis, Ioannis, Palani, Ayad, Calik, Gokhan, Kulaksiz, Deniz, Simanaviciene, Vaida, Simopoulou, Mara, Güngör, Nur Dokuzeylül, Blecher, Gideon, Falcone, Marco, Jezek, Davor, Preto, Mirko, Amar, Edouard, Le, Tan, Ahn, Sun Tae, Rezano, Andri, Singh, Keerti, Rocco, Lucia, Savira, Missy, Rajmil, Osvaldo, Darbandi, Sara, Sogutdelen, Emrullah, Boeri, Luca, Hernández, Guadalupe, Hakim, Lukman, Morimoto, Yoshiharu, Japari, Andrian, Sofikitis, Nikolaos, Altay, Baris, Metin Mahmutoglu, Asli, Al Hashimi, Manaf, Ziouziou, Imad, Anagnostopoulou, Christina, Lin, Haocheng, Shah, Rupin

المساهمون: Cleveland Clinic, Sohag University, King Abdulaziz University, Minia University, Polytechnic University of Marche Ancona, Italy / Università Politecnica delle Marche Ancona, Italia (UNIVPM), CHI Poissy-Saint-Germain, Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Case Western Reserve University Cleveland, Faculty of Medicine Selcuk University Konya, Turkey, Selçuk University = Selçuk Üniversitesi, Università degli studi di Catania = University of Catania (Unict), Universiti Teknologi MARA Shah Alam (UiTM ), Vattikuti Urology Institute, Henry Ford Hospital, Cipto Mangunkusumo Hospital Jakarta, University of Medicine Pham Ngoc Thach, McGill University = Université McGill Montréal, Canada, Hamad medical corporation, Weill Cornell Medicine Qatar, Cairo University, Cliniques Universitaires Saint-Luc Bruxelles, Flinders University of South Australia, Ankara University School of Medicine Turkey, Loma Linda University, University of Santo Tomas Manila, Philippines, Imperial College London, University of the Western Cape (UWC), Biruni University Istanbul (BU), King Fahad Medical City Riyadh (KFMC), Juntendo University Hospital Tokyo, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Lithuanian University of Health Science (LUSH), Başkent University Hospital Adana, Turkey, Università degli Studi di Foggia = University of Foggia (Unifg), Qatar University, Tehran University of Medical Sciences (TUMS), Royan Institute for Reproductive Biomedicine Tehran, Iran, Kansai Medical University, Pusan National University (PNU), Sultan Qaboos University (SQU), King Faisal Specialist Hospital and Research Center, Vietnam National University Hanoï (VNU), Innsbruck Medical University = Medizinische Universität Innsbruck (IMU), University of Kent Canterbury, King Abdulaziz Medical City, The University of Queensland (UQ All campuses : Brisbane, Dutton Park Gatton, Herston, St Lucia and other locations ), Hope Generation Foundation, Osaka University Osaka, Hôpital militaire d'instruction Mohammed V Rabat, Maroc, Istanbul Medipol University, University of Virginia School of Medicine, Southern Illinois University School of Medicine, Ankara Üniversitesi, Hamad Medical Corporation Doha, Qatar, McGovern Medical School Houston, TX, USA (McGMS), The University of Texas Health Science Center at Houston (UTHealth), MD Anderson Cancer Center Houston, الجامعة اللبنانية بيروت = Lebanese University Beirut = Université libanaise Beyrouth (LU / ULB), Al-Zahraa Hospital University Medical Center (ZHUMC), Department of Biological Sciences Toledo, University of Toledo, University of Bucharest (UniBuc), Istanbul University, Università degli Studi di Roma "La Sapienza" = Sapienza University Rome (UNIROMA), Lilavati Hospital and Research Centre, Algiers University I, Aristotle University of Thessaloniki, University of Garmian, National and Kapodistrian University of Athens (NKUA), Bahcesehir University Istanbul, Monash University Melbourne, Molinette Hospital, Zagreb University Hospital, American Hospital of Paris, Universitas Padjadjaran (UnPad), The University of the West Indies, Università degli studi della Campania "Luigi Vanvitelli" = University of the Study of Campania Luigi Vanvitelli, Fundació Puigvert Barcelona, Spain, Universitas Airlangga (UNAIR), University of Ioannina, Ege University Izmir, Bozok University, Yozgat, Burjeel Hospital, Peking University Beijing

المصدر: ISSN: 2287-4208.

مصطلحات موضوعية: Diagnostic test, Infertility, male, Survey, DNA fragmentation, Delphi method, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology

الوصف: International audience ; Purpose: Sperm DNA fragmentation (SDF) is a functional sperm abnormality that can impact reproductive potential, for which four assays have been described in the recently published sixth edition of the WHO laboratory manual for the examination and processing of human semen. The purpose of this study was to examine the global practices related to the use of SDF assays and investigate the barriers and limitations that clinicians face in incorporating these tests into their practice.Materials and methods: Clinicians managing male infertility were invited to complete an online survey on practices related to SDF diagnostic and treatment approaches. Their responses related to the technical aspects of SDF testing, current professional society guidelines, and the literature were used to generate expert recommendations via the Delphi method. Finally, challenges related to SDF that the clinicians encounter in their daily practice were captured.Results: The survey was completed by 436 reproductive clinicians. Overall, terminal deoxynucleotidyl transferase deoxyuridine triphosphate Nick-End Labeling (TUNEL) is the most commonly used assay chosen by 28.6%, followed by the sperm chromatin structure assay (24.1%), and the sperm chromatin dispersion (19.1%). The choice of the assay was largely influenced by availability (70% of respondents). A threshold of 30% was the most selected cut-off value for elevated SDF by 33.7% of clinicians. Of respondents, 53.6% recommend SDF testing after 3 to 5 days of abstinence. Although 75.3% believe SDF testing can provide an explanation for many unknown causes of infertility, the main limiting factors selected by respondents are a lack of professional society guideline recommendations (62.7%) and an absence of globally accepted references for SDF interpretation (50.3%).Conclusions: This study represents the largest global survey on the technical aspects of SDF testing as well as the barriers encountered by clinicians. Unified global recommendations regarding clinician ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37635341; hal-04196414; https://hal.inrae.fr/hal-04196414Test; PUBMED: 37635341; WOS: 001063965600001

الإتاحة: https://doi.org/10.5534/wjmh.230076Test
https://hal.inrae.fr/hal-04196414Test

المؤلفون: GRECO, Ermanno, FRANCO, Giorgio, F. Scarselli, M. Iacobelli, L. Rienzi, Fubaldi, S. Ferrero, N. Anniballo, C. Mendoza, I. Tesarik

المساهمون: Greco, Ermanno, F., Scarselli, M., Iacobelli, L., Rienzi, Fubaldi, S., Ferrero, Franco, Giorgio, N., Anniballo, C., Mendoza, I., Tesarik

مصطلحات موضوعية: dna fragmentation, ejaculated spermatozoa, icsi, sperm fertilizing ability, testicular spermatozoa

الوصف: BACKGROUND: Sperm DNA damage (fragmentation) is a recently discovered cause of male infertility for which no efficient treatment has yet been found. Previous findings have suggested that clinically relevant sperm DNA damage may occur at the post-testicular level. This study was undertaken to assess the clinical usefulness of ICSI with testicular spermatozoa in this indication. METHODS: The percentage of spermatozoa with fragmented DNA, assessed by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labelling assay, and ICSI outcomes were compared in two sequential attempts performed, respectively, with ejaculated and testicular spermatozoa in 18 men with increased sperm DNA fragmentation. RESULTS: The incidence of DNA fragmentation was markedly lower in testicular spermatozoa as compared with ejaculated spermatozoa. No differences in fertilization and cleavage rates and in embryo morphological grade were found between the ICSI attempts performed with ejaculated and with testicular spermatozoa. However, eight ongoing clinical pregnancies (four singleton and four twin) were achieved by ICSI with testicular spermatozoa (44.4% pregnancy rate; 20.7% implantation rate), whereas ICSI with ejaculated spermatozoa led to only one pregnancy which was spontaneously aborted. CONCLUSIONS: These data show that ICSI with testicular spermatozoa provides the first efficient assisted reproduction treatment option for men with high levels of sperm DNA damage.

وصف الملف: STAMPA

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/15539441; info:eu-repo/semantics/altIdentifier/wos/WOS:000226199000033; volume:20; issue:1; firstpage:226; lastpage:230; numberofpages:5; journal:HUMAN REPRODUCTION; http://hdl.handle.net/11573/115925Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-19944433572; http://PUBMEDTest; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000226199000033&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396aTest; http://www.scopus.com/inward/record.url?eid=2-s2.0-19944433572&partnerID=65&md5=a72cbf266032ab8d02af025e4eec9475Test

الإتاحة: https://doi.org/10.1093/humrep/deh590Test
http://hdl.handle.net/11573/115925Test
http://PUBMEDTest
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000226199000033&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396aTest
http://www.scopus.com/inward/record.url?eid=2-s2.0-19944433572&partnerID=65&md5=a72cbf266032ab8d02af025e4eec9475Test

المؤلفون: Agarwal, A, Farkouh, A, Saleh, R, Abdel-Meguid Hamoda, TA-A, Harraz, AM, Kavoussi, P, Arafa, M, Salvio, G, Rambhatla, A, Toprak, T, Gül, M, Phuoc, NHV, Boitrelle, F, Birowo, P, Ghayda, RA, Cannarella, R, Kuroda, S, Durairajanayagam, D, Zini, A, Wyns, C, Sarikaya, S, Tremellen, K, Mostafa, T, Sokolakis, I, Evenson, DP, Henkel, R, Zohdy, W, Chung, E, Ziouziou, I, Falcone, M, Russo, GI, Al-Hashimi, M, Calogero, AE, Ko, E, Colpi, G, Lewis, S, Serefoglu, EC, Bahar, F, Martinez, M, Nguyen, Q, Ambar, RF, Bakircioglu, ME, Kandil, H, Mogharabian, N, Sabbaghian, M, Taniguchi, H, Tsujimura, A, Sajadi, H, Ibrahim, W, Atmoko, W, Vogiatzi, P, Gunes, S, Gilani, MAS, Roychoudhury, S, Güngör, ND, Hakim, L, Adriansjah, R, Kothari, P, Jindal, S, Amar, E, Park, HJ, Long, TQT, Homa, S, Karthikeyan, VS, Zilaitiene, B, Rosas, IM, Marino, A, Pescatori, E, Ozer, C, Akhavizadegan, H, Garrido, N, Busetto, GM, Adamyan, A, Al-Marhoon, M, Elbardisi, H, Dolati, P, Darbandi, M, Darbandi, S, Balercia, G, Pinggera, G-M, Micic, S, Ho, CCK, Moussa, M, Preto, M, Zenoaga-Barbăroșie, C, Smith, RP, Kosgi, R, Rosette, JDL, El-Sakka, AI, Abumelha, SM, Mierzwa, TC, Ong, TA, Banihani, SA, Bowa, K, Fukuhara, S, Boeri, L, Danacıoğlu, YO, Gokalp, F, Selim, OM, Cho, C-L, Tadros, NN, Ugur, MR, Ozkent, MS, Chiu, P, Kalkanli, A, Khalafalla, K, Vishwakarma, RB, Finocchi, F, Andreadakis, S, Giulioni, C, Çeker, G, Ceyhan, E, Malhotra, V, Yilmaz, M, Timpano, M, Barrett, TL, Kim, SHK, Ahn, S-T, Giacone, F, Palani, A, Duarsa, GWK, Kadioglu, A, Gadda, F, Zylbersztejn, DS, Aydos, K, Kulaksız, D, Gupte, D, Calik, G, Karna, KK, Drakopoulos, P, Baser, A, Kumar, V, Molina, JMC, Rajmil, O, Ferreira, RH, Leonardi, S, Avoyan, A, Sogutdelen, E, Franco, G, Ramsay, J, Ramirez, L, Shah, R, Global Andrology Forum

المساهمون: Agarwal, Ashok, Farkouh, Ala'A, Saleh, Ramadan, Abdel-Meguid Hamoda, Taha Abo-Almagd, Harraz, Ahmed M, Kavoussi, Parviz, Arafa, Mohamed, Salvio, Gianmaria, Rambhatla, Amarnath, Toprak, Tuncay, Gül, Murat, Phuoc, Nguyen Ho Vinh, Boitrelle, Florence, Birowo, Ponco, Ghayda, Ramy Abou, Cannarella, Rossella, Kuroda, Shinnosuke, Durairajanayagam, Damayanthi, Zini, Armand, Wyns, Christine, Sarikaya, Selcuk, Tremellen, Kelton, Mostafa, Taymour, Sokolakis, Ioanni, Evenson, Donald P, Henkel, Ralf, Zohdy, Wael, Chung, Eric, Ziouziou, Imad, Falcone, Marco, Russo, Giorgio I, Al-Hashimi, Manaf, Calogero, Aldo E, Ko, Edmund, Colpi, Giovanni, Lewis, Sheena, Serefoglu, Ege Can, Bahar, Fahmi, Martinez, Marlon, Nguyen, Quang, Ambar, Rafael F, Bakircioglu, Mustafa Emre, Kandil, Hussein, Mogharabian, Nasser, Sabbaghian, Marjan, Taniguchi, Hisanori, Tsujimura, Akira, Sajadi, Hesamoddin, Ibrahim, Wael, Atmoko, Widi, Vogiatzi, Paraskevi, Gunes, Sezgin, Gilani, Mohammad Ali Sadighi, Roychoudhury, Shubhadeep, Güngör, Nur Dokuzeylül, Hakim, Lukman, Adriansjah, Ricky, Kothari, Priyank, Jindal, Sunil, Amar, Edouard, Park, Hyun Jun, Long, Tran Quang Tien, Homa, Sheryl, Karthikeyan, Vilvapathy Senguttuvan, Zilaitiene, Birute, Rosas, Israel Maldonado, Marino, Angelo, Pescatori, Edoardo, Ozer, Cevahir, Akhavizadegan, Hamed, Garrido, Nicola, Busetto, Gian Maria, Adamyan, Aram, Al-Marhoon, Mohamed, Elbardisi, Haitham, Dolati, Parisa, Darbandi, Mahsa, Darbandi, Sara, Balercia, Giancarlo, Pinggera, Germar-Michael, Micic, Sava, Ho, Christopher Chee Kong, Moussa, Mohamad, Preto, Mirko, Zenoaga-Barbăroșie, Cătălina, Smith, Ryan P, Kosgi, Raghavender, Rosette, Jean de la, El-Sakka, Ahmed I, Abumelha, Saad Mohammed, Mierzwa, Tiago Cesar, Ong, Teng Aik, Banihani, Saleem A, Bowa, Kasonde, Fukuhara, Shinichiro, Boeri, Luca, Danacıoğlu, Yavuz Onur, Gokalp, Fatih, Selim, Osama Mohamed, Cho, Chak-Lam, Tadros, Nicholas N, Ugur, Muhammet Rasit, Ozkent, Mehmet Serkan, Chiu, Peter, Kalkanli, Arif, Khalafalla, Kareim, Vishwakarma, Ranjit B, Finocchi, Federica, Andreadakis, Sotiri, Giulioni, Carlo, Çeker, Gökhan, Ceyhan, Erman, Malhotra, Vineet, Yilmaz, Mehmet, Timpano, Massimiliano, Barrett, Trenton L, Kim, Shannon Hee Kyung, Ahn, Sun-Tae, Giacone, Filippo, Palani, Ayad, Duarsa, Gede Wirya Kusuma, Kadioglu, Ate, Gadda, Franco, Zylbersztejn, Daniel Suslik, Aydos, Kaan, Kulaksız, Deniz, Gupte, Deepak, Calik, Gokhan, Karna, Keshab Kumar, Drakopoulos, Panagioti, Baser, Aykut, Kumar, Vijay, Molina, Juan Manuel Corral, Rajmil, Osvaldo, Ferreira, Raphael H, Leonardi, Sofia, Avoyan, Armen, Sogutdelen, Emrullah, Franco, Giorgio, Ramsay, Jonathan, Ramirez, Liliana, Shah, Rupin, Rocco, Lucia

مصطلحات موضوعية: Male infertility, Practice guideline, Delphi method, DNA fragmentation, Survey, Sperm

الوصف: PURPOSE: Sperm DNA fragmentation (SDF) testing was recently added to the sixth edition of the World Health Organization laboratory manual for the examination and processing of human semen. Many conditions and risk factors have been associated with elevated SDF; therefore, it is important to identify the population of infertile men who might benefit from this test. The purpose of this study was to investigate global practices related to indications for SDF testing, compare the relevant professional society guideline recommendations, and provide expert recommendations. MATERIALS AND METHODS: Clinicians managing male infertility were invited to take part in a global online survey on SDF clinical practices. This was conducted following the CHERRIES checklist criteria. The responses were compared to professional society guideline recommendations related to SDF and the appropriate available evidence. Expert recommendations on indications for SDF testing were then formulated, and the Delphi method was used to reach consensus. RESULTS: The survey was completed by 436 experts from 55 countries. Almost 75% of respondents test for SDF in all or some men with unexplained or idiopathic infertility, 39% order it routinely in the work-up of recurrent pregnancy loss (RPL), and 62.2% investigate SDF in smokers. While 47% of reproductive urologists test SDF to support the decision for varicocele repair surgery when conventional semen parameters are normal, significantly fewer general urologists (23%; p=0.008) do the same. Nearly 70% would assess SDF before assisted reproductive technologies (ART), either always or for certain conditions. Recurrent ART failure is a common indication for SDF testing. Very few society recommendations were found regarding SDF testing. CONCLUSIONS: This article presents the largest global survey on the indications for SDF testing in infertile men, and demonstrates diverse practices. Furthermore, it highlights the paucity of professional society guideline recommendations. Expert recommendations are proposed to help guide clinicians.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::21c6308f43cbd338be7c3d0122b0ee10Test
https://hdl.handle.net/11591/497179Test