التفاصيل البيبلوغرافية
| العنوان: |
Nuclear caspase-3 and capase-7 activation, and Poly(ADP-ribose) polymerase cleavage are early events in camptothecin-induced apoptosis. |
| المؤلفون: |
Rodríguez-Hernández, Á., Brea-Calvo, G., Fernández-Ayala, D. J. M., Cordero, M., Navas, P., Sánchez-Alcázar, J. A. |
| المصدر: |
Apoptosis; Jan2006, Vol. 11 Issue 1, p131-139, 9p, 7 Diagrams, 1 Graph |
| مصطلحات موضوعية: |
APOPTOSIS, CAMPTOTHECIN, ADENOSINE diphosphate, POLYMERASE chain reaction, CYTOCHROME c, DNA damage |
| مستخلص: |
Chemotherapy-induced apoptosis by DNA-damaging drugs is thought to be generally dependent on the release of cytochrome c and the subsequent activation of caspase-9 and -3. However, the molecular mechanism of how damaged DNA triggers the apoptotic process is not clear. To better understand the mechanisms underlying this process, we examined drug-induced apoptosis in cultured H-460 cells. Using cell fractionation, western blotting, and immunofluorescence assays, we show that the activation of nuclear caspases-7 and -3, and poly(ADP-ribose) polymerase (PARP) cleavage, are early events in camptothecin-induced apoptosis. Moreover, we demonstrate that these events precede the release of cytochrome c and apoptotic inducing factor, and the activation of caspases 2, 8, 9 and 12. Together our results suggest that drugs acting at the DNA level can initiate apoptosis via nuclear caspase activation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
