دورية أكاديمية
DNA damage reduces heterogeneity and coherence of chromatin motions
| العنوان: | DNA damage reduces heterogeneity and coherence of chromatin motions |
|---|---|
| المؤلفون: | Locatelli, Maëlle, Lawrimore, Josh, Lin, Hua, Sanaullah, Sarvath, Seitz, Clayton, Segall, Dave, Kefer, Paul, Moreno, Naike Salvador, Lietz, Benton, Anderson, Rebecca, Holmes, Julia, Yuan, Chongli, Holzwarth, George, Bloom, Kerry S., Liu, Jing, Bonin, Keith, Vidi, Pierre-Alexandre |
| المساهمون: | Physics, School of Science |
| المصدر: | PMC |
| بيانات النشر: | National Academy of Science |
| سنة النشر: | 2022 |
| المجموعة: | Indiana University - Purdue University Indianapolis: IUPUI Scholar Works |
| مصطلحات موضوعية: | Structured illumination, Chromatin mobility, Heterogeneity, DNA damage, Phase separation |
| الوصف: | Chromatin motions depend on and may regulate genome functions, in particular the DNA damage response. In yeast, DNA double-strand breaks (DSBs) globally increase chromatin diffusion, whereas in higher eukaryotes the impact of DSBs on chromatin dynamics is more nuanced. We mapped the motions of chromatin microdomains in mammalian cells using diffractive optics and photoactivatable chromatin probes and found a high level of spatial heterogeneity. DNA damage reduces heterogeneity and imposes spatially defined shifts in motions: Distal to DNA breaks, chromatin motions are globally reduced, whereas chromatin retains higher mobility at break sites. These effects are driven by context-dependent changes in chromatin compaction. Photoactivated lattices of chromatin microdomains are ideal to quantify microscale coupling of chromatin motion. We measured correlation distances up to 2 µm in the cell nucleus, spanning chromosome territories, and speculate that this correlation distance between chromatin microdomains corresponds to the physical separation of A and B compartments identified in chromosome conformation capture experiments. After DNA damage, chromatin motions become less correlated, a phenomenon driven by phase separation at DSBs. Our data indicate tight spatial control of chromatin motions after genomic insults, which may facilitate repair at the break sites and prevent deleterious contacts of DSBs, thereby reducing the risk of genomic rearrangements. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | Proceedings of the National Academy of Sciences of the United States of America; Locatelli M, Lawrimore J, Lin H, et al. DNA damage reduces heterogeneity and coherence of chromatin motions. Proc Natl Acad Sci U S A. 2022;119(29):e2205166119. doi:10.1073/pnas.2205166119; https://hdl.handle.net/1805/36477Test |
| الإتاحة: | https://doi.org/10.1073/pnas.2205166119Test https://hdl.handle.net/1805/36477Test |
| حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 International ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.BEF798BE |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |