Evaluation of microsatellite sequence-tagged site markers for characterizing Vitis vinifera cultivars
| العنوان: | Evaluation of microsatellite sequence-tagged site markers for characterizing Vitis vinifera cultivars |
|---|---|
| المؤلفون: | Botta, R., Scott, N. S., Eynard, I., Thomas, M. R. |
| بيانات النشر: | Vitis / Bundesanstalt Zuechtungsforschung:Forschung Wein Geilweilerhof, D 76833 Siebeldingen Germany:011 49 6345 41141, EMAIL: doku-vitis@bafz.de, Fax: 011 49 6345 919050, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | molecular markers, fungi, cultivar identification, food and beverages, grapevine, DNA, microsatellite analysis, STMS, database |
| الوصف: | Twenty four cultivars and clones from Italian germplasm collections were DNA typed for the four sequence-tagged microsatellite sites VVS1-FP, VVS2-JOE, VVS5-FP and VV29-TAM. The analysis was performed using primers labelled with different fluorochromes and using a GENESCAN apparatus. The results were compared with data obtained for samples from Australian collections. By comparing the combined genotype of the studied loci all the cultivars could be singularly distinguished except Favorita, Pigato and Vermentino, thus supporting the hypothesis that all three are the same cultivar. Cultivars common to Italian and Australian germplasm collections were found to have the same genotype indicating that the study of microsatellites as sequence-tagged site (STS) markers is a suitable universal system for worldwide grapevine cultivar identification. Application of the semi-automated GENESCAN system made the analysis of microsatellite STS markers fast and reproducible between laboratories. VITIS - Journal of Grapevine Research, Vol. 34 No. 2 (1995): Vitis |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::333327423d7e12085ffcc7fdc60bb2a7Test http://hdl.handle.net/2318/3289Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....333327423d7e12085ffcc7fdc60bb2a7 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |