دورية أكاديمية
The effect of extracorporeal shock wave on joint capsule fibrosis based on A2AR-Nrf2/HO-1 pathway in a rat extending knee immobilization model
| العنوان: | The effect of extracorporeal shock wave on joint capsule fibrosis based on A2AR-Nrf2/HO-1 pathway in a rat extending knee immobilization model |
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| المؤلفون: | Hai Yuan, Kui Wang, Quan-Bing Zhang, Feng Wang, Yun Zhou |
| المصدر: | Journal of Orthopaedic Surgery and Research, Vol 18, Iss 1, Pp 1-12 (2023) |
| بيانات النشر: | BMC |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Joint capsule fibrosis, Extracorporeal shock wave therapy, Adenosine A2A receptor, Neurotrophic factor e2-related factor 2, Heme oxidase-1, Orthopedic surgery, RD701-811, Diseases of the musculoskeletal system, RC925-935 |
| الوصف: | Joint capsule fibrosis, a common complication of joint immobilization, is mainly characterized by abnormal collagen deposition. The present study aimed to investigate the effect of extracorporeal shock wave therapy (ESWT) on reduced collagen deposition in the joint capsule during immobilization-induced joint capsule fibrosis. Additionally, the potential involvement of the adenosine A2A receptor (A2AR)-Neurotrophic factor e2-related factor 2 (Nrf2)/Haem oxygenase-1 (HO-1) pathway was explored. Thirty 3-month-old male Sprague–Dawley rats were randomly assigned to five groups: control (C), immobilization model (IM), natural recovery (NR), ESWT intervention (EI), and ESWT combined with A2AR antagonist SCH 58261 intervention (CI). After the left knee joints of rats in the IM, NR, EI and CI groups were immobilized using a full-extension fixation brace for 4 weeks, the EI and CI groups received ESWT twice a week for 4 weeks. The CI group was also treated with ESWT following intraperitoneal injection of SCH 58261 (0.01 mg/kg) for 4 weeks. The range of motion of the left knee joint was measured, and the protein levels of collagens I and III, A2AR, phosphorylated-protein kinase A/protein kinase A (p-PKA/PKA), p-Nrf2/Nrf2, and HO-1 were analysed by Western blotting. The IM and NR groups showed significantly greater arthrogenic contracture than the C group (P < 0.05). Compared to the NR group, the EI and CI groups exhibited significant improvement in arthrogenic contracture (P < 0.05). Conversely, the EI group showed lower contracture than the CI group (P < 0.05). Similar results were observed for collagen deposition and the protein levels of collagens I and III. The intervention groups (EI and CI groups) showed higher levels of p-Nrf2/Nrf2 and HO-1 than the NR group (P < 0.05). Moreover, the EI group exhibited higher levels of p-PKA/PKA, p-Nrf2/Nrf2, and HO-1 than the CI group (P < 0.05). However, no significant difference was found in the A2AR levels among the five groups (P > 0.05). ESWT may ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-7-89492-593-0 7-89492-593-4 |
| تدمد: | 1749-799X |
| العلاقة: | https://doi.org/10.1186/s13018-023-04420-1Test; https://doaj.org/toc/1749-799XTest; https://doaj.org/article/b7894925934041648ce70c463fbb43fcTest |
| DOI: | 10.1186/s13018-023-04420-1 |
| الإتاحة: | https://doi.org/10.1186/s13018-023-04420-1Test https://doaj.org/article/b7894925934041648ce70c463fbb43fcTest |
| رقم الانضمام: | edsbas.2059B48E |
| قاعدة البيانات: | BASE |
Full Text Finder | ردمك: | 9787894925930 7894925934 |
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| تدمد: | 1749799X |
| DOI: | 10.1186/s13018-023-04420-1 |