التفاصيل البيبلوغرافية
| العنوان: |
Genomic Analysis of Group B Streptococcus from Neonatal Sepsis Reveals Clonal CC17 Expansion and Virulence- and Resistance-Associated Traits After Intrapartum Antibiotic Prophylaxis. |
| المؤلفون: |
Zhou, Yan, Wang, Lin-Qi, Yan, Qing, Lee, Chien-Chung, Hsu, Mei-Hua, Liao, Wan-Ting, Zhang, Liang, Chiu, Cheng-Hsun |
| المصدر: |
Clinical Infectious Diseases; 12/15/2022, Vol. 75 Issue 12, p2153-2160, 8p |
| مصطلحات موضوعية: |
NEONATAL sepsis, SEQUENCE analysis, SINGLE nucleotide polymorphisms, STREPTOCOCCAL diseases, STREPTOCOCCUS, ANTIBIOTIC prophylaxis, SEROTYPES, GENOMICS, GENOTYPES, DISEASE susceptibility, AMPICILLIN, MICROBIAL virulence, DRUG resistance in microorganisms, INTRAPARTUM care, MICROBIAL sensitivity tests |
| مستخلص: |
Background Group B Streptococcus (GBS) is a leading cause of invasive neonatal infections. This study aimed to investigate the trend of GBS serotype and genotype change and their correlation with antimicrobial resistance before and after implementation of intrapartum antibiotic prophylaxis (IAP). Methods We performed serotyping, whole-genome sequencing, antimicrobial susceptibility testing, and single-nucleotide polymorphism (SNP)–based phylogenetic analysis on 238 invasive GBS isolates collected from October 1998 to February 2020 in Taiwan. Results There were 7 serotypes and 6 clonal complexes (CCs) among the 238 GBS isolates, and more than half of the isolates carried multiple antimicrobial resistance genes. The expansion of CC17 strains and the increase in late-onset disease occurred synchronously after the implementation of IAP. Analysis of the carriage isolates from pregnant women showed diverse serotype distribution in the IAP era. The antimicrobial susceptibility testing showed that all 238 strains were susceptible to ampicillin and penicillin, while the number of various resistance genes in GBS genomes was found increased with the expansion of CC17. Compared with reference genomes, 697 nonsynonymous SNPs in 443 protein-coding genes were CC17 specific. Conclusions The study revealed the expansion of GBS CC17 and the increase of late-onset disease that occurred simultaneously with the implementation of IAP. Although the susceptibility of CC17 to antimicrobial agents is not different from that of other sequence types at present, GBS with phenotypic resistance to antimicrobials may emerge in the future, given the environmental selection pressure and the continued accumulation of SNP mutations. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
