دورية أكاديمية
Bone marrow stromal cell regeneration profile in treated B-Cell precursor acute lymphoblastic leukemia patients: association with MRD status and patient outcome
| العنوان: | Bone marrow stromal cell regeneration profile in treated B-Cell precursor acute lymphoblastic leukemia patients: association with MRD status and patient outcome |
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| المؤلفون: | Oliveira, Elen, Costa, Elaine S., Ciudad, Juana, Gaipa, Giuseppe, Sedek, Lukasz, Barrena, Susana, Szczepanski, Tomasz, Buracchi, Chiara, Silvestri, Daniela, Siqueira, Patrícia F. R., Mello, Fabiana V., Torres, Rafael C., Oliveira, Leonardo M. R., Fay-Neves, Isabelle V. C., Sonneveld, Edwin, Velden, Vincent H. J. van der, Mejstrikova, Ester, Ribera, Josep-Maria, Conter, Valentino, Schrappe, Martin, Dongen, J. J. M. van, Land, Marcelo G. P., Orfao, Alberto |
| المساهمون: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundaçao Capes (Brasil), Ministerio de Educación, Cultura y Deporte (España), Centro de Investigación Biomédica en Red Cáncer (España), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute |
| سنة النشر: | 2022 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | B-cell precursor acute lymphoblastic leukemia, Stromal cells, Mesenchymal stem cells, Endothelial cells, Bone marrow microenvironment, Multiparameter flow cytometry, Disease free survival, Measurable residual disease |
| الوصف: | For the last two decades, measurable residual disease (MRD) has become one of the most powerful independent prognostic factors in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). However, the effect of therapy on the bone marrow (BM) microenvironment and its potential relationship with the MRD status and disease free survival (DFS) still remain to be investigated. Here we analyzed the distribution of mesenchymal stem cells (MSC) and endothelial cells (EC) in the BM of treated BCP-ALL patients, and its relationship with the BM MRD status and patient outcome. For this purpose, the BM MRD status and EC/MSC regeneration profile were analyzed by multiparameter flow cytometry (MFC) in 16 control BM (10 children; 6 adults) and 1204 BM samples from 347 children and 100 adult BCP-ALL patients studied at diagnosis (129 children; 100 adults) and follow-up (824 childhood samples; 151 adult samples). Patients were grouped into a discovery cohort (116 pediatric BCP-ALL patients; 338 samples) and two validation cohorts (74 pediatric BCP-ALL, 211 samples; and 74 adult BCP-ALL patients; 134 samples). Stromal cells (i.e., EC and MSC) were detected at relatively low frequencies in all control BM (16/16; 100%) and in most BCP-ALL follow-up samples (874/975; 90%), while they were undetected in BCP-ALL BM at diagnosis. In control BM samples, the overall percentage of EC plus MSC was higher in children than adults (p = 0.011), but with a similar EC/MSC ratio in both groups. According to the MRD status similar frequencies of both types of BM stromal cells were detected in BCP-ALL BM studied at different time points during the follow-up. Univariate analysis (including all relevant prognostic factors together with the percentage of stromal cells) performed in the discovery cohort was used to select covariates for a multivariate Cox regression model for predicting patient DFS. Of note, an increased percentage of EC (>32%) within the BCP-ALL BM stromal cell compartment at day +78 of therapy emerged as an independent unfavorable ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | Publisher's version; http://dx.doi.org/10.3390/cancers14133088Test; Sí; e-issn: 2072-6694; Cancers 14(13): 3088 (2022); http://hdl.handle.net/10261/296476Test; http://dx.doi.org/10.13039/501100003593Test; http://dx.doi.org/10.13039/501100004587Test; http://dx.doi.org/10.13039/501100003176Test; http://dx.doi.org/10.13039/501100003329Test; http://dx.doi.org/10.13039/501100002322Test |
| DOI: | 10.3390/cancers14133088 |
| DOI: | 10.13039/501100003593 |
| DOI: | 10.13039/501100004587 |
| DOI: | 10.13039/501100003176 |
| DOI: | 10.13039/501100003329 |
| DOI: | 10.13039/501100002322 |
| الإتاحة: | https://doi.org/10.3390/cancers14133088Test https://doi.org/10.13039/501100003593Test https://doi.org/10.13039/501100004587Test https://doi.org/10.13039/501100003176Test https://doi.org/10.13039/501100003329Test https://doi.org/10.13039/501100002322Test http://hdl.handle.net/10261/296476Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.17E0BB56 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/cancers14133088 |
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