Comparison and Sensitivity Evaluation of Three Different Commercial Real-Time Quantitative PCR Kits for SARS-CoV-2 Detection
| العنوان: | Comparison and Sensitivity Evaluation of Three Different Commercial Real-Time Quantitative PCR Kits for SARS-CoV-2 Detection |
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| المؤلفون: | Marija Vukcevic, Dragana Despot, Ana Loncar, Gordana Petrovic, Danijela Miljanovic, Ana Banko, Andja Cirkovic |
| المصدر: | Viruses, Vol 13, Iss 1321, p 1321 (2021) Viruses Volume 13 Issue 7 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Diagnostic methods, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Biology, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Microbiology, Article, 03 medical and health sciences, 0302 clinical medicine, PCR kits, Virology, Humans, 030212 general & internal medicine, diagnostic efficacy, molecular testing, SARS-CoV-2, COVID-19, Gold standard (test), Molecular biology, QR1-502, 3. Good health, Reverse transcription polymerase chain reaction, 030104 developmental biology, Infectious Diseases, Real-time polymerase chain reaction, Cross-Sectional Studies, COVID-19 Nucleic Acid Testing, Detection performance, RNA, Viral, Reagent Kits, Diagnostic, Primer (molecular biology), real-time PCR, Serbia |
| الوصف: | Real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most sensitive and specific assay and, therefore, is the “gold standard” diagnostic method for the diagnosis of SARS-CoV-2 infection. The aim of this study was to compare and analyze the detection performance of three different commercially available SARS-CoV-2 nucleic acid detection kits: Sansure Biotech, GeneFinderTM, and TaqPathTM on 354 randomly selected samples from hospitalized COVID-19 patients. All PCR reactions were performed using the same RNA isolates and one real-time PCR machine. The final result of the three evaluated kits was not statistically different (p = 0.107), and also had a strong positive association and high Cohen’s κ coefficient. In contrast, the average Ct values that refer to the ORF1ab and N gene amplification were significantly different (p < 0.001 and p < 0.001, respectively), with the lowest obtained by the TaqPathTM for the ORF1ab and by the Sansure Biotech for the N gene. The results show a high similarity in the analytical sensitivities for SARS-CoV-2 detection, which indicates that the diagnostic accuracy of the three assays is comparable. However, the SanSure Biotech kit showed a bit better diagnostic performance. Our findings suggest that the imperative for improvement should address the determination of cut-off Ct values and rapid modification of the primer sets along with the appearance of new variants. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65a087cc63b4798fc08b22f063c56601Test https://www.mdpi.com/1999-4915/13/7/1321Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....65a087cc63b4798fc08b22f063c56601 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
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