دورية أكاديمية
Differential therapeutic effect of extracellular vesicles derived by bone marrow and adipose mesenchymal stem cells on wound healing of diabetic ulcers and correlation to their cargoes
| العنوان: | Differential therapeutic effect of extracellular vesicles derived by bone marrow and adipose mesenchymal stem cells on wound healing of diabetic ulcers and correlation to their cargoes |
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| المؤلفون: | Pomatto M., Gai C., Negro F., Cedrino M., Grange C., Ceccotti E., Togliatto G., Collino F., Tapparo M., Figliolini F., Lopatina T., Brizzi M. F., Camussi G. |
| المساهمون: | M. Pomatto, C. Gai, F. Negro, M. Cedrino, C. Grange, E. Ceccotti, G. Togliatto, F. Collino, M. Tapparo, F. Figliolini, T. Lopatina, M.F. Brizzi, G. Camussi |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | Adipose, Bone marrow, Cargo, Diabete, Exosome, Extracellular vesicle, Mesenchymal, MSC, Therapy, Wound healing, Adipose Tissue, Animal, Bone Marrow Cell, Diabetes Complication, Flow Cytometry, Gene Expression Profiling, Immunohistochemistry, Mesenchymal Stem Cell, Mice, Ulcer, Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio, Settore MED/04 - Patologia Generale |
| الوصف: | Extracellular vesicles (EVs) derived from mesenchymal stem cells isolated from both bone marrow (BMSCs) and adipose tissue (ADSCs) show potential therapeutic effects. These vesicles often show a similar beneficial effect on tissue regeneration, but in some contexts, they exert different biological properties. To date, a comparison of their molecular cargo that could explain the different biological effect is not available. Here, we demonstrated that ADSC-EVs, and not BMSC-EVs, promote wound healing on a murine model of diabetic wounds. Besides a general similarity, the bioinformatic analysis of their protein and miRNA cargo highlighted important differences between these two types of EVs. Molecules present exclusively in ADSC-EVs were highly correlated to angiogenesis, whereas those expressed in BMSC-EVs were preferentially involved in cellular proliferation. Finally, in vitro analysis confirmed that both ADSC and BMSC-EVs exploited beneficial effect on cells involved in skin wound healing such as fibroblasts, keratinocytes and endothelial cells, but through different cellular processes. Consistent with the bioinformatic analyses, BMSC-EVs were shown to mainly promote proliferation, whereas ADSC-EVs demonstrated a major effect on angiogenesis. Taken together, these results provide deeper comparative information on the cargo of ADSC-EVs and BMSC-EVs and the impact on regenerative processes essential for diabetic wound healing. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/33917759; info:eu-repo/semantics/altIdentifier/wos/WOS:000644332000001; volume:22; issue:8; firstpage:1; lastpage:26; numberofpages:26; journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; http://hdl.handle.net/2434/855880Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85103820943 |
| DOI: | 10.3390/ijms22083851 |
| الإتاحة: | https://doi.org/10.3390/ijms22083851Test http://hdl.handle.net/2434/855880Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C01583E3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms22083851 |
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