التفاصيل البيبلوغرافية
| العنوان: |
TRANSFER OF PLATELET-DERIVED GROWTH FACTOR-BB GENE BY GENE GUN INCREASES CONTRACTION OF COLLAGEN LATTICE BY FIBROBLASTS IN DIABETIC AND NON-DIABETIC HUMAN SKIN. |
| المؤلفون: |
Kuwahara, Hiroaki, Mitchell, Alfred T., MacKlin, Michael D., Zhao, Jiangang, Listengarten, Dmitry, Phillips, Linda G. |
| المصدر: |
Scandinavian Journal of Plastic & Reconstructive Surgery & Hand Surgery; Nov2000, Vol. 34 Issue 4, p301-307, 7p, 4 Charts, 4 Graphs |
| مصطلحات موضوعية: |
PLATELET-derived growth factor, GENETIC transformation |
| مستخلص: |
We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGFBB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
