المؤلفون: Abdelgayed M. Younes, Mohamed S. Hassanane, Dalia M. Mabrouk, Mahmoud A. Mahmoud, Karima F. Mahrous, Alkhateib Y. Gaafar, Wagdy K. B. Khalil, Heba A.M. Abd El-Kader, Mohamad M. Aboelenin

المصدر: Developmental and comparative immunology. 112

مصطلحات موضوعية: 0301 basic medicine, Fish Proteins, Vibrio anguillarum, food.ingredient, Immunology, Pseudomonas fluorescens, Microbiology, 03 medical and health sciences, Nile tilapia, Fish Diseases, food, Gene expression, Animals, Streptococcus iniae, biology, Tilapia, 04 agricultural and veterinary sciences, Bacterial Infections, biology.organism_classification, Up-Regulation, Oreochromis, 030104 developmental biology, Aeromonas, Organ Specificity, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Transcriptome, Developmental Biology, Antimicrobial Cationic Peptides

الوصف: The antimicrobial activity of tilapia piscidin 4 (TP4) was determined in vitro against four bacterial strains, Aeromonas hydrophilla, Pseudomonas fluorescens, Streptococcus iniae and Vibrio anguillarum. Nile tilapia were infected with low and high doses of the tested pathogens; after 3, 6, 24 h and 7 days of the specific TP4 gene expression, tissue immunolocalization was also performed. Histopathological examination revealed septicaemia and necrosis of hemopoietic tissue for all of the tested bacteria. Immunolocalization showed abundance in S. iniae-infected fish tissues. Quantitative RT-PCR analysis revealed that high doses raised mRNA expression levels compared to low doses and expression levels increased in the infected fish, particularly after 24 h, indicating that TP4 exerts potent bactericidal activity against some fish pathogens and plays an essential role in fish immunity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8052f3995f13137e6e384a901f3cc97Test
https://pubmed.ncbi.nlm.nih.gov/32634526Test

المؤلفون: Mohamed S. Hassanane, El-Sayed A.M. Abd Alla, Aida I. El-Makawy

المصدر: Reproduction, nutrition, development. 41(1)

مصطلحات موضوعية: Male, Embryology, Ratón, Medicine (miscellaneous), Mitosis, Biology, medicine.disease_cause, Andrology, chemistry.chemical_compound, Mice, Pregnancy, Spermatocytes, medicine, Animals, Estrogens, Non-Steroidal, Mycotoxin, Zearalenone, Genetics, Chromosome Aberrations, Toxin, Embryo, Embryo, Mammalian, Teratology, Teratogens, Reproductive Medicine, chemistry, Toxicity, Animal Science and Zoology, Female, Genotoxicity, Developmental Biology, Food Science

الوصف: Genotoxic effects of the mycotoxin Zearalenone (ZEN) were evaluated on albino mice. The investigation was assessed using 4 criteria: chromosome aberrations in bone marrow and sper- matocytes of adult male mice; chromosome analysis and teratological effects of mice embryos. Zear- alenone was administrated to both adult males and pregnant females with 2 doses level (5 μg.kg -1 and 10 μg.kg -1 ZEN). Zearalenone was found to reduce the mitotic activity in treated males and the embryos proving that it is a cytotoxic substance. In treated males and females, it induced some chro- mosome abnormalities with no significant increase over the control at the doses investigated, except for some few figures. Similar results were observed for the teratological study. The results in general could consider zearalenone as a toxic mycotoxin for both adult animals and embryos. It is highly rec- ommended that a great attention should be paid towards the toxicity of zearalenone to mono-gastric animals and human, especially it contaminate corn that is widely used in human and animal feeding. genotoxicity / zearalenone / mice / embryo / chromosome / spermatocyte

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::daceaf89756b12191921956b399651e5Test
https://pubmed.ncbi.nlm.nih.gov/11368247Test