دورية أكاديمية
A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts.
| العنوان: | A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts. |
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| المؤلفون: | Nobis, Max, Herrmann, David, Warren, Sean C, Kadir, Shereen, Leung, Wilfred, Killen, Monica, Magenau, Astrid, Stevenson, David, Lucas, Morghan C, Reischmann, Nadine, Vennin, Claire, Conway, James RW, Boulghourjian, Alice, Zaratzian, Anaiis, Law, Andrew M, Gallego-Ortega, David, Ormandy, Christopher J, Walters, Stacey N, Grey, Shane T, Bailey, Jacqueline, Chtanova, Tatyana, Quinn, Julian MW, Baldock, Paul A, Croucher, Peter I, Schwarz, Juliane P, Mrowinska, Agata, Zhang, Lei, Herzog, Herbert, Masedunskas, Andrius, Hardeman, Edna C, Gunning, Peter W, Del Monte-Nieto, Gonzalo, Harvey, Richard P, Samuel, Michael S, Pajic, Marina, McGhee, Ewan J, Johnsson, Anna-Karin E, Sansom, Owen J, Welch, Heidi CE, Morton, Jennifer P, Strathdee, Douglas, Anderson, Kurt I, Timpson, Paul |
| بيانات النشر: | Elsevier BV //dx.doi.org/10.1016/j.celrep.2017.09.022 Cell Rep |
| سنة النشر: | 2017 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | FLIM-FRET, actin, biosensors, breast cancer, cell biology, development, immunology, intravital imaging, pancreatic cancer, small GTPase RhoA, Animals, Antineoplastic Agents, Biosensing Techniques, Bone and Bones, Cell Movement, Dasatinib, Erlotinib Hydrochloride, Female, Fluorescence Resonance Energy Transfer, Gene Expression Regulation, Intestine, Small, Intravital Microscopy, Mammary Glands, Animal, Mammary Neoplasms, Experimental, Mechanotransduction, Cellular, Mice |
| الوصف: | The small GTPase RhoA is involved in a variety of fundamental processes in normal tissue. Spatiotemporal control of RhoA is thought to govern mechanosensing, growth, and motility of cells, while its deregulation is associated with disease development. Here, we describe the generation of a RhoA-fluorescence resonance energy transfer (FRET) biosensor mouse and its utility for monitoring real-time activity of RhoA in a variety of native tissues in vivo. We assess changes in RhoA activity during mechanosensing of osteocytes within the bone and during neutrophil migration. We also demonstrate spatiotemporal order of RhoA activity within crypt cells of the small intestine and during different stages of mammary gestation. Subsequently, we reveal co-option of RhoA activity in both invasive breast and pancreatic cancers, and we assess drug targeting in these disease settings, illustrating the potential for utilizing this mouse to study RhoA activity in vivo in real time. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Print; application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/294102Test |
| DOI: | 10.17863/CAM.41203 |
| الإتاحة: | https://doi.org/10.17863/CAM.41203Test https://www.repository.cam.ac.uk/handle/1810/294102Test |
| حقوق: | Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.7DDB94F1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.41203 |
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