التفاصيل البيبلوغرافية
| العنوان: |
Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes. |
| المؤلفون: |
Tang, Li Hua1,2, Tan, Qi1, Bao, Da Peng1, Zhang, Xue Hong2, Jian, Hua Hua2, Li, Yan1, Yang, Rui heng1, Wang, Ying1 |
| المصدر: |
BioMed Research International. 10/27/2016, Vol. 2016, p1-8. 8p. |
| مصطلحات موضوعية: |
*FUNGI physiology, *PROTEIN analysis, *PROTEIN metabolism, *COMPARATIVE studies, *ELECTROPHORESIS, *MASS spectrometry, *MUSHROOMS, *OXIDATION-reduction reaction, *POLYMERASE chain reaction, *PROTEINS, *RESEARCH funding, *TRANSFERASES, *PROTEOMICS, *OXIDATIVE stress, *DATA analysis software, *DESCRIPTIVE statistics |
| مستخلص: |
Light-induced brown film (BF) formation by the vegetative mycelium of Lentinula edodes is important for ensuring the quantity and quality of this edible mushroom. Nevertheless, the molecular mechanism underlying this phenotype is still unclear. In this study, a comparative proteomic analysis of mycelial BF formation in L. edodes was performed. Seventy-three protein spots with at least a twofold difference in abundance on two-dimensional electrophoresis (2DE) maps were observed, and 52 of them were successfully identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF/MS). These proteins were classified into the following functional categories: small molecule metabolic processes (39%), response to oxidative stress (5%), and organic substance catabolic processes (5%), followed by oxidation-reduction processes (3%), single-organism catabolic processes (3%), positive regulation of protein complex assembly (3%), and protein metabolic processes (3%). Interestingly, four of the proteins that were upregulated in response to light exposure were nucleoside diphosphate kinases. To our knowledge, this is the first proteomic analysis of the mechanism of BF formation in L. edodes. Our data will provide a foundation for future detailed investigations of the proteins linked to BF formation. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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