التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of a Liquid Media MALDI-TOF MS Protocol for the Identification of Dermatophytes Isolated from Tinea capitis Infections |
| المؤلفون: |
Lecerf, Pauline, De Paepe, Roelke, Jazaeri, Yasaman, Normand, Anne Cécile, Martiny, Delphine, Packeu, Ann |
| المصدر: |
Journal of Fungi, 8 (12 |
| سنة النشر: |
2022 |
| المجموعة: |
DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
| مصطلحات موضوعية: |
Généralités, dermatophytes, fungus, in-house library, liquid culture, MALDI-TOF MS, tinea capitis |
| الوصف: |
One of the most common types of tinea is the superficial infection of the hair and scalp area known as tinea capitis. It is responsible for frequent outbreaks in nurseries and schools and represents a global health problem. Correct identification of the infection agent is essential in the determination of the infection source, epidemiological course, and treatment initiation. The conventional identification methods (direct exam, culture, DNA sequencing) are time-consuming, require experienced staff, are time-consuming, and the latter is expensive for routine identifications. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is gaining new ground for routine identification of filamentous fungi. The main advantages of MALDI-TOF MS are its rapid and accurate identification capability, relatively low cost, and easy integration into the laboratory routine. Its accuracy heavily depends on the quality of the reference spectra database. Identification of clinical isolates with MALDI-TOF MS protocol requires a sub-culturing step to ensure reliable identification. It can take days to weeks before fungal growth appears on solid medium. In this study, a unique MALDI-TOF MS protocol using liquid cultures of dermatophyte species was developed in order to shorten the turnaround time for the culture and identification of clinical isolates. Material and Method A standard MALDI-TOF MS protocol was adapted for liquid instead of solid cultures. Three different databases were tested. Results Using the liquid media MALDI-TOF MS protocol, a global rate of 62% correct identification (RCI) was obtained, compared with 87% for the protocol based on solid cultures. Trichophyton tonsurans was not correctly identified in all isolates using liquid cultures, with 88% of the isolates misidentified as Trichophyton interdigitale. The turnaround time for primary isolates for the solid and liquid protocols were respectively 11.7 and 11.6 days (no significant difference between both methods (p = 0.96)). ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
1 full-text file(s): application/pdf |
| اللغة: |
English |
| العلاقة: |
uri/info:doi/10.3390/jof8121248; uri/info:scp/85149524295; https://dipot.ulb.ac.be/dspace/bitstream/2013/371078/1/doi_354722.pdfTest; http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/371078Test |
| الإتاحة: |
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/371078Test |
| رقم الانضمام: |
edsbas.563BDD14 |
| قاعدة البيانات: |
BASE |
