دورية أكاديمية
Denoising of high-resolution single-particle electron-microscopy density maps by their approximation using three-dimensional Gaussian functions
| العنوان: | Denoising of high-resolution single-particle electron-microscopy density maps by their approximation using three-dimensional Gaussian functions |
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| المؤلفون: | Jonić, S., Vargas, J., Melero, R., Gómez-Blanco, J., Carazo, J.M., Sorzano, C.O.S. |
| المساهمون: | Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de recherche pour le développement IRD : UR206-Centre National de la Recherche Scientifique (CNRS), Biocomputing Unit Madrid |
| المصدر: | ISSN: 1047-8477. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Electron microscopy density map, single particle analysis, denoising, macromolecular complexes, structure, Gaussian functions, [INFO.INFO-BT]Computer Science [cs]/Biotechnology |
| الوصف: | International audience ; Cryo-electron microscopy (cryo-EM) of frozen-hydrated preparations of isolated macromolecular complexes is the method of choice to obtain the structure of complexes that cannot be easily studied by other experimental methods due to their flexibility or large size. An increasing number of macromolecular structures are currently being obtained at subnanometer resolution but the interpretation of structural details in such EM-derived maps is often difficult because of noise at these high-frequency signal components that reduces their contrast. In this paper, we show that the method for EM density-map approximation using Gaussian functions can be used for denoising of single-particle EM maps of high (typically subnanometer) resolution. We show its denoising performance using simulated and experimental EM density maps of several complexes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-01303768; https://hal.sorbonne-universite.fr/hal-01303768Test; https://hal.sorbonne-universite.fr/hal-01303768/documentTest; https://hal.sorbonne-universite.fr/hal-01303768/file/Jonic_Denoising_of.pdfTest |
| DOI: | 10.1016/j.jsb.2016.04.007 |
| الإتاحة: | https://doi.org/10.1016/j.jsb.2016.04.007Test https://hal.sorbonne-universite.fr/hal-01303768Test https://hal.sorbonne-universite.fr/hal-01303768/documentTest https://hal.sorbonne-universite.fr/hal-01303768/file/Jonic_Denoising_of.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.35ABE5D5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jsb.2016.04.007 |
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