التفاصيل البيبلوغرافية
| العنوان: |
EGF-coated nano-dendriplexes for tumor-targeted nucleic acid delivery in vivo. |
| المؤلفون: |
Li, Jun, Chen, Lei, Liu, Nan, Li, Shengnan, Hao, Yanli, Zhang, Xiaoning |
| المصدر: |
Drug Delivery; Jun2016, Vol. 23 Issue 5, p1718-1725, 8p |
| مصطلحات موضوعية: |
NUCLEIC acids, EPIDERMAL growth factor, DENDRIMERS synthesis, GENE delivery techniques, CANCER treatment, THERAPEUTICS |
| مستخلص: |
The clinical success of therapeutic DNA is still hindered due to the lack of effective delivery carriers. Here, we designed a tumor-targeted gene nano delivery system based on EGFR targeting strategy. Epidermal growth factor (EGF) was introduced to nano-complexes of PAMAM dendrimer and DNA via electrostatic interactions to form self-assembled PAMAM/DNA/EGF nano-complexes. The properties of self-assembled complexes were characterized by gel retardation assay and particle size and zeta potential analysis. Meanwhile, the toxicity of EGF-dendriplexes was evaluated by the MTT assay, which indicated that the complexes exhibited decreased cytotoxicity with the incorporation of EGF. We labeled polyamidoamine (PAMAM) dendrimers with FITC or a near-infrared (NIR) dye Lss670 and tested the cellular uptakein vitroand biodistribution in xenograft mouse tumor models. As compared to dendriplexes, the ternary EGF-dendriplexes showed a significantly higher cellular uptake into HepG2 cells due to the specific binding between EGF and EGF receptor (EGFR) over expressed on HepG2 cells, which resulted in the enhanced gene transfection efficiency. The biodistribution of EGF-dendriplexesin vivowas monitored within vivoimaging technique, which indicated that EGF-dendriplexes enhanced EGFR-positive tumor-targeted biodistribution. These findings indicate that this novel nano-vector realized efficiently tumor-targeting gene delivery and high efficient gene expressionin vivo, and it may possess a potential targeting gene delivery system in cancer therapy. [ABSTRACT FROM PUBLISHER] |
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| قاعدة البيانات: |
Complementary Index |
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