Abdominal γ-radiation induces an accumulation of function-impaired regulatory t cells in the small intestine
| العنوان: | Abdominal γ-radiation induces an accumulation of function-impaired regulatory t cells in the small intestine |
|---|---|
| المؤلفون: | Fabienne Billiard, Marc Benderitter, Valérie Buard, Christine Linard |
| المساهمون: | Institut de RadioProtection et de Sureté Nucléaire, Direction de la Radioprotection de l’Homme |
| المصدر: | International Journal of Radiation Oncology Biology Physics International Journal of Radiation Oncology Biology Physics, 2011, 80 (3), pp.869-876. ⟨10.1016/j.ijrobp.2010.12.041⟩ |
| بيانات النشر: | HAL CCSD, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Cancer Research, Cytotoxic, cell infiltration, T-Lymphocytes, [SDV]Life Sciences [q-bio], Messenger, Abdominal irradiation, Immunostaining, Mouse models, Small, Inbred C57BL, T-Lymphocytes, Regulatory, Seepage, Immune tolerance, In-vitro, Mice, 0302 clinical medicine, regulatory T lymphocyte, Transforming Growth Factor beta, Intestine, Small, Abdomen, Mesenteric lymph nodes, Cytotoxic T cell, CTLA-4 Antigen, Mesentery, monoclonal antibody CD28, Lymph node, Functional characteristics, 0303 health sciences, Immunity, Cellular, Radiation, biology, messenger RNA, article, FOXP3, hemic and immune systems, Forkhead Transcription Factors, Regulatory, 3. Good health, Interleukin-10, Intestine, CD, medicine.anatomical_structure, Lymphatic system, Body fluids, Oncology, priority journal, 030220 oncology & carcinogenesis, immunohistochemistry, In-control, in vitro study, T cells, transcription factor FOXP3, chemical and pharmacologic phenomena, Radiation Dosage, Peripheral tissue, 03 medical and health sciences, Antigens, CD, medicine, Immune Tolerance, Animals, Radiology, Nuclear Medicine and imaging, RNA, Messenger, Radiation-induced, Antigens, CD3 antibody, protein expression, mouse, 030304 developmental biology, Soil mechanics, Immune tolerances, Tissue, nonhuman, cytotoxic T lymphocyte antigen 4, business.industry, gamma radiation, Immunity, Transforming growth factor beta, Small intestine, Coculture, Effector cells, Mice, Inbred C57BL, mesentery lymph node, Gamma Rays, Immunology, Cancer research, biology.protein, RNA, Irradiation, Cell culture, Cellular, Lymph Nodes, interleukin 10, business |
| الوصف: | Purpose: To assess the frequency and the functional characteristics of one major component of immune tolerance, the CD4+FoxP3+ regulatory T cells (Tregs) in a mouse model of abdominal irradiation. Methods and Materials: Mice were exposed to a single abdominal dose of γ-radiation (10 Gy). We evaluated small intestine Treg infiltration by Foxp3 immunostaining and the functional suppressive activity of Tregs isolated from mesenteric lymph nodes. Results: Foxp3 immunostaining showed that radiation induced a long-term infiltration of the intestine by Tregs (levels 5.5 times greater than in controls). Co-culture of Tregs from mesenteric lymph nodes with CD4+ effector cells showed that the Tregs had lost their suppressive function. This loss was associated with a significant decrease in the levels of Foxp3, TGF-β, and CTLA-4 mRNA, all required for optimal Treg function. At Day 90 after irradiation, Tregs regained their suppressive activity as forkhead box P3 (Foxp3), transforming growth factor beta (TGF-β), and cytotoxic T-lymphocyte antigen 4 (CTLA-4) expression returned to normal. Analysis of the secretory function of mesenteric lymph node Tregs, activated in vitro with anti-CD3/anti-CD28 Abs, showed that this dysfunction was independent of a defect in interleukin-10 secretion. Conclusion: Radiation caused a long-term accumulation of function-impaired Foxp3+CD4+ Tregs in the intestine. Our study provides new insights into how radiation affects the immune tolerance in peripheral tissues. © 2011 Elsevier Inc. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::467a05e8989bbbe8dca82e46d3821767Test https://hal.archives-ouvertes.fr/hal-02954934Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....467a05e8989bbbe8dca82e46d3821767 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |