التفاصيل البيبلوغرافية
| العنوان: |
Cross-Omics Comparison of Stress Responses in Mesothelial Cells Exposed to Heat- versus Filter-Sterilized Peritoneal Dialysis Fluids. |
| المؤلفون: |
Kratochwill, Klaus, Bender, Thorsten O., Lichtenauer, Anton M., Herzog, Rebecca, Tarantino, Silvia, Bialas, Katarzyna, Jörres, Achim, Aufricht, Christoph |
| المصدر: |
BioMed Research International; 10/1/2015, Vol. 2015, p1-12, 12p |
| مصطلحات موضوعية: |
PERITONEAL dialysis, CYTOPROTECTION, HEAT shock proteins, GEL electrophoresis, GENE expression, WESTERN immunoblotting, CYTOTOXINS, MESSENGER RNA, BODY fluids, ELECTROPHORESIS, PSYCHOLOGICAL stress, GENE expression profiling |
| مستخلص: |
Recent research suggests that cytoprotective responses, such as expression of heat-shock proteins, might be inadequately induced in mesothelial cells by heat-sterilized peritoneal dialysis (PD) fluids. This study compares transcriptome data and multiple protein expression profiles for providing new insight into regulatory mechanisms. Two-dimensional difference gel electrophoresis (2D-DIGE) based proteomics and topic defined gene expression microarray-based transcriptomics techniques were used to evaluate stress responses in human omental peritoneal mesothelial cells in response to heat- or filter-sterilized PD fluids. Data from selected heat-shock proteins were validated by 2D western-blot analysis. Comparison of proteomics and transcriptomics data discriminated differentially regulated protein abundance into groups depending on correlating or noncorrelating transcripts. Inadequate abundance of several heat-shock proteins following exposure to heat-sterilized PD fluids is not reflected on the mRNA level indicating interference beyond transcriptional regulation. For the first time, this study describes evidence for posttranscriptional inadequacy of heat-shock protein expression by heat-sterilized PD fluids as a novel cytotoxic property. Cross-omics technologies introduce a novel way of understanding PDF bioincompatibility and searching for new interventions to reestablish adequate cytoprotective responses. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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