المؤلفون: Bollyky, Paul L., Wu, Rebecca P., Falk, Ben A., Lord, James D., Long, S. Alice, Preisinger, Anton, Teng, Brandon, Holt, Gregory E., Standifer, Nathan E., Braun, Kathleen R., Xie, Cindy Fang, Samuels, Peter L., Vernon, Robert B., Gebe, John A., Wight, Thomas N., Nepom, Gerald T., Germain, Ron

المصدر: Proceedings of the National Academy of Sciences of the United States of America, 2011 May 01. 108(19), 7938-7943.

الوصول الحر: https://www.jstor.org/stable/41242290Test

المؤلفون: Reichstetter, Sandra bkwok@benaroyaresearch.org, Standifer, Nathan E., Geubtner, Kelly A., Liu, Andrew W., Agar, Stacy L., Kwok, William W.¹ sreichstetter@earthlink.net

المصدر: Immunology. Mar2006, Vol. 117 Issue 3, p350-357. 8p. 2 Charts, 8 Graphs.

مصطلحات موضوعية: *HERPES simplex, *MAJOR histocompatibility complex, *TRANSPLANTATION of organs, tissues, etc., *IMMUNOREGULATION, *NUCLEOTIDE sequence, *T-cell receptor genes, *CYTOKINES, *T cells

مستخلص: Alloreactivity is one of the most serious problems in organ transplantation. It has been hypothesized that pre-existing alloreactive T cells are actually cross-reacting cells that have been primed by the autologous major histocompatibility complex (MHC) and a specific peptide. CD8+ cytotoxic T lymphocytes that are alloreactive and recognize a virus-peptide that is presented by the autologous MHC have been reported. Here we demonstrate a cross-reactivity that exists between DQ0602 restricted, herpes simplex type 2 VP16 40–50 specific CD4+ T-cell clones, which can be alloreactive to DQ0601. Though most of the DQ0602 restricted T-cell clones we isolated from two different donors were not alloreactive, weakly cross-reacting T-cell clones could be isolated from both donors. Two strongly cross-reacting T-cell clones with high affinity interaction of their T-cell receptor (TCR) with both DQ0602/VP16 40–50 and DQ0601 could be isolated from one donor. DNA sequencing of the a fragment of the Vβ gene used in their TCR confirmed that these two T cells indeed are two independent clones. These clones are cytotoxic and produce cytokines of a T helper 2-like pattern. Possible implications in a DR-matched transplantation setting are discussed. [ABSTRACT FROM AUTHOR]