دورية أكاديمية
Induction of interleukin-10 and suppressor of cytokine signalling-3 gene expression following peptide immunotherapy
| العنوان: | Induction of interleukin-10 and suppressor of cytokine signalling-3 gene expression following peptide immunotherapy |
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| المؤلفون: | Tarzi, M., Klunker, S., Texier, C., Verhoef, A., Stapel, S. O., Akdis, C. A., Maillere, B., Kay, A. B., Larché, M. |
| المساهمون: | Imperial College London (Department of Allergy εt Clinical Immunology), Faculty of Medicine-National Heart εt Lung Institute, Swiss Institute of Allergy and Asthma Research (SIAF), Universität Zürich Zürich (UZH), Commissariat a l'Energie Atomique-Saclay (Protein Engineering and Research Department), Commissariat à l'Energie Atomique-Saclay |
| المصدر: | ISSN: 0954-7894. |
| بيانات النشر: | HAL CCSD Wiley |
| سنة النشر: | 2006 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | Immunopathology, Immunology, Transcription factor, Anergy, Tolerance, T-Lymphocyte, Human, Allergy, Antigenic determinant, Treatment, Immunotherapy, Peptides, Gene expression, Cytokine, Signal transduction, Suppressor gene, Interleukin 10, MESH: Adult, MESH: Bee Venoms, MESH: Humans, MESH: Immunoglobulin G, MESH: Immunohistochemistry, MESH: Immunotherapy, Active, MESH: Injections, Intradermal, MESH: Interleukin-10, MESH: Interleukin-13, MESH: Leukocytes, Mononuclear |
| الوصف: | International audience ; BACKGROUND: Allergen-derived (T cell epitope) peptides may be safer for immunotherapy than native allergen, as they do not cross-link immunoglobulin (Ig)E. However, HLA polymorphism results in multiple potential epitopes. Synthetic peptides of phospholipase (PL) A(2) were selected for a peptide vaccine, on the basis of binding affinity for commonly expressed HLA-DR molecules. OBJECTIVE: To evaluate treatment with an HLA-DR-based PLA(2) peptide vaccine in subjects with mild honeybee allergy in an open, controlled study. METHODS: Twelve volunteers with allergy to bee venom received nine intradermal injections of PLA(2) peptides, with six untreated subjects serving as controls. Outcome was assessed by the size of the late-phase cutaneous reaction to allergen, peripheral blood mononuclear cell (PBMC) proliferation, cytokine release, and expression of genes associated with immune regulation. RESULTS: Subjects receiving peptides showed a decrease in the magnitude of the late-phase cutaneous reaction to bee venom compared with controls (P=0.03). The proliferation of venom-stimulated PBMCs decreased in treated subjects compared with controls (P=0.01). Peptide treatment reduced the production of IL-13 by PLA(2)-stimulated PBMCs (P<0.01) and IFN-gamma (P<0.01), and increased the production of IL-10 (P=0.02). Transcription of the suppressor of cytokine signalling (Socs)3 gene was significantly increased following therapy. A transient, but modest, increase in allergen-specific IgG was also observed. CONCLUSION: HLA-DR-based T cell epitopes modify surrogate markers associated with successful immunotherapy and induction of immune regulation, supporting the concept that this form of treatment may be efficacious in human allergic disease. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/16630151; hal-00529931; https://hal.archives-ouvertes.fr/hal-00529931Test; PUBMED: 16630151 |
| DOI: | 10.1111/j.1365-2222.2006.02469.x |
| الإتاحة: | https://doi.org/10.1111/j.1365-2222.2006.02469.xTest https://hal.archives-ouvertes.fr/hal-00529931Test |
| رقم الانضمام: | edsbas.CB3BEAFC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-2222.2006.02469.x |
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