Mucosal-Associated Invariant T Cells Are Depleted and Exhibit Altered Chemokine Receptor Expression and Elevated Granulocyte Macrophage-Colony Stimulating Factor Production During End-Stage Renal Disease
| العنوان: | Mucosal-Associated Invariant T Cells Are Depleted and Exhibit Altered Chemokine Receptor Expression and Elevated Granulocyte Macrophage-Colony Stimulating Factor Production During End-Stage Renal Disease |
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| المؤلفون: | Jennifer A. Juno, Jillian L. M. Waruk, Kathleen M. Wragg, Christine Mesa, Carmen Lopez, Joe Bueti, Stephen J. Kent, T. Blake Ball, Sandra A. Kiazyk |
| المصدر: | Frontiers in Immunology Frontiers in Immunology, Vol 9 (2018) |
| بيانات النشر: | Frontiers Media SA, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Receptor expression, medicine.medical_treatment, C-C chemokine receptor type 6, Lymphocyte Activation, 0302 clinical medicine, Immunology and Allergy, Cells, Cultured, Original Research, end-stage renal disease, Middle Aged, Phenotype, Granulocyte macrophage colony-stimulating factor, medicine.anatomical_structure, Cytokine, Cytokines, Female, Receptors, Chemokine, medicine.drug, lcsh:Immunologic diseases. Allergy, Adult, Immunology, Mucosal associated invariant T cell, Biology, Lymphocyte Depletion, Mucosal-Associated Invariant T Cells, Immunophenotyping, End stage renal disease, 03 medical and health sciences, Immune system, latent tuberculosis, medicine, granulocyte macrophage-colony stimulating factor, Humans, mucosal associated invariant T cells, Aged, Monocyte, Granulocyte-Macrophage Colony-Stimulating Factor, 030104 developmental biology, Gene Expression Regulation, inflammation, Leukocytes, Mononuclear, Kidney Failure, Chronic, lcsh:RC581-607, Biomarkers, Interferon-gamma Release Tests, CD161, 030215 immunology |
| الوصف: | Background End-stage renal disease (ESRD) is associated with an increased susceptibility to infectious diseases, including infection with Mycobacterium tuberculosis (Mtb). Mucosal-associated invariant T (MAIT) cells recognize vitamin B metabolites produced by many bacterial species, including Mtb, and may play an important role in providing protective immunity against tuberculosis infection in the lung. To date, little is known about MAIT cell frequency, phenotype, or function in ESRD patients. Methods MAIT cells, identified by surface marker expression or MR1 tetramer binding, were characterized in 20 ESRD and 20 healthy control participants by multicolor flow cytometry. Ex vivo MAIT cell phenotype and cytokine production following PMA/ionomycin, IL-12/IL-18, or Escherichia coli stimulation were determined. Monocyte phenotype and plasma C-reactive protein/inflammatory cytokine levels were quantified by flow cytometry, ELISA, and multiplex bead array. Results Peripheral blood MAIT cells were significantly depleted among ESRD patients compared to controls by both phenotypic and tetramer analysis and exhibited a loss of CXCR3 expression coupled to increased expression of CCR6 and CXCR6. ESRD was also associated with a shift in MAIT PMA-induced cytokine production away from IFNγ production and toward granulocyte macrophage-colony stimulating factor (GM-CSF) secretion, and a loss of E. coli-stimulated tumor necrosis factor α expression. Loss of IFNγ expression was associated with a combination of age, alterations in Tbet and Eomes expression, and inflammatory plasma cytokine levels. Conclusion The loss of peripheral blood MAIT cells and associated shifts in tissue homing receptor expression and GM-CSF production may contribute to an immune environment that is permissive to bacterial replication, particularly in the lungs. |
| تدمد: | 1664-3224 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6497d5b008b9fead932408825eef4a9Test https://doi.org/10.3389/fimmu.2018.01076Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f6497d5b008b9fead932408825eef4a9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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