دورية أكاديمية
Soluble fibrinogen-like protein 2/fibroleukin exhibits immunosuppressive properties: Suppressing T cell proliferation and inhibiting maturation of bone marrow-derived dendritic cells
| العنوان: | Soluble fibrinogen-like protein 2/fibroleukin exhibits immunosuppressive properties: Suppressing T cell proliferation and inhibiting maturation of bone marrow-derived dendritic cells |
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| المؤلفون: | Lakatoo, S, Rotstein, O, Cattral, M, Gorczynski, RM, Levy, GA, Zhang, L, Young, KJ, Chan, MWC, Khadaroo, RG, Kay, LS, Chan, CWY |
| بيانات النشر: | //www.jimmunol.org United States |
| سنة النشر: | 2003 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | Animals, Antigens, Cd80 - Biosynthesis, Apoptosis - Immunology, Baculoviridae - Genetics, Bone Marrow Cells - Cytology - Immunology - Metabolism, Cell Differentiation - Immunology, Cells, Cultured, Cytokines - Biosynthesis, Dendritic Cells - Cytology - Immunology - Metabolism, Female, Fibrinogen - Biosynthesis - Genetics - Metabolism - Physiology, Genetic Vectors, Growth Inhibitors - Biosynthesis - Genetics - Metabolism - Physiology, Histocompatibility Antigens Class Ii - Biosynthesis, Lymphocyte Activation - Immunology, Lymphocyte Culture Test, Mixed, Mice, Inbred A, Inbred Balb C, Protein Binding - Immunology, Solubility, Suppressor Factors, Immunologic - Biosynthesis - Genetics - Metabolism - Physiology, T-Lymphocytes - Cytology - Immunology - Metabolism, Th2 Cells - Immunology - Metabolism, Thromboplastin - Biosynthesis - Genetics - Metabolism - Physiology |
| الوصف: | Fibrinogen-like protein 2 (fgl2)/fibroleukin is a member of the fibrinogen-related protein superfamily. In addition to its established role in triggering thrombosis, it is known to be secreted by T cells. The soluble fgl2 (sfgl2) protein generated in a baculovirus expression system bound to both T cells and bone marrow-derived dendritic cells (DC) in a specific manner. sfgl2 exhibited immunomodulatory properties capable of inhibiting T cell proliferation stimulated by alloantigens, anti-CD3/anti-CD28 mAbs, and Con A in a dose-dependent manner; however, it had no inhibitory effects on CTL activity. The time- and dose-dependent inhibitory effect of sfgl2 on alloreactive T cell proliferation could be neutralized by a mAb against mouse fgl2. Polarization toward a Th2 cytokine profile with decreased production of IL-2 and IFN-γ and increased production of IL-4 and IL-10 was observed in sfgl2-treated allogeneic cultures. Exposure of immature DC to sfgl2 abrogated the expression of CD80high and MHC class IIhigh molecules and markedly inhibited NF-κB nuclear translocation, thus inhibiting their maturation. sFgl2-treated DC had an impaired ability to stimulate allogeneic T cell proliferation. Maximal inhibition of proliferation was observed when allogeneic T cells were cultured with sfgl2-treated DC and sfgl2 protein was added in the culture. These data provide the first evidence to demonstrate that sfgl2 exerts immunosuppressive effects on T cell proliferation and DC maturation. ; link_to_subscribed_fulltext |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0022-1767 |
| العلاقة: | Journal of Immunology; http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037446535&selection=ref&src=s&origin=recordpageTest; Journal Of Immunology, 2003, v. 170 n. 8, p. 4036-4044; 4044; WOS:000182171100013; eid_2-s2.0-0037446535; 4036; http://hdl.handle.net/10722/149614Test; 170 |
| الإتاحة: | http://hdl.handle.net/10722/149614Test |
| رقم الانضمام: | edsbas.D7CD7E6E |
| قاعدة البيانات: | BASE |
| تدمد: | 00221767 |
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