دورية أكاديمية
BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells
| العنوان: | BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells |
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| المؤلفون: | Janssen, Sanne Marlijn, Moscona, Roy, Elchebly, Mounib, Papadakis, Andreas Ioannis, Redpath, Margaret, Wang, Hangjun, Rubin, Eitan, van Kempen, Léon Cornelis, Spatz, Alan |
| المصدر: | Janssen , S M , Moscona , R , Elchebly , M , Papadakis , A I , Redpath , M , Wang , H , Rubin , E , van Kempen , L C & Spatz , A 2020 , ' BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells ' , Cell Death and Discovery , vol. 6 , no. 1 , 1 . https://doi.org/10.1038/s41420-019-0235-xTest |
| سنة النشر: | 2020 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | DIFFERENTIAL EXPRESSION ANALYSIS, CANCER-TESTIS GENE, SIGNALING PATHWAY, IMPRINTED SITES, BORIS, CTCF, METHYLATION, DEREPRESSION, CTCFL/BORIS, REGULATOR |
| الوصف: | Melanoma is among the most aggressive cancers due to its tendency to metastasize early. Phenotype switching between a proliferative and an invasive state has been suggested as a critical process for metastasis, though the mechanisms that regulate state transitions are complex and remain poorly understood. Brother of Regulator of Imprinted Sites (BORIS), also known as CCCTC binding factor-Like (CTCFL), is a transcriptional modulator that becomes aberrantly expressed in melanoma. Yet, the role of BORIS in melanoma remains elusive. Here, we show that BORIS is involved in melanoma phenotype switching. Genetic modification of BORIS expression in melanoma cells combined with whole-transcriptome analysis indicated that BORIS expression contributes to an invasion-associated transcriptome. In line with these findings, inducible BORIS overexpression in melanoma cells reduced proliferation and increased migration and invasion, demonstrating that the transcriptional switch is accompanied by a phenotypic switch. Mechanistically, we reveal that BORIS binds near the promoter of transforming growth factor-beta 1 (TFGB1), a well-recognized factor involved in the transition towards an invasive state, which coincided with increased expression of TGFB1. Overall, our study indicates a pro-invasive role for BORIS in melanoma via transcriptional reprogramming. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://research.rug.nl/en/publications/4c970ba5-dab9-4625-bb82-0778dcf682a0Test |
| DOI: | 10.1038/s41420-019-0235-x |
| الإتاحة: | https://doi.org/10.1038/s41420-019-0235-xTest https://hdl.handle.net/11370/4c970ba5-dab9-4625-bb82-0778dcf682a0Test https://research.rug.nl/en/publications/4c970ba5-dab9-4625-bb82-0778dcf682a0Test https://pure.rug.nl/ws/files/123709394/BORIS_CTCFL_promotes_a_switch_from_a_proliferative_towards_an_invasive_phenotype_in_melanoma_cells.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.EF2BD44A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41420-019-0235-x |
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