دورية أكاديمية
Cryptoccocal meningitis in Yaoundé (Cameroon) HIV infected patients: Diagnosis, frequency and Cryptococcus neoformans isolates susceptibility study to fluconazole
| العنوان: | Cryptoccocal meningitis in Yaoundé (Cameroon) HIV infected patients: Diagnosis, frequency and Cryptococcus neoformans isolates susceptibility study to fluconazole |
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| المؤلفون: | Kammalac Ngouana, T., Dongtsa, J., Kouanfack, C., Tonfack, C., Fomena, S., Mallié, M., Delaporte, E., Boyom, F.-Fekam, Bertout, S. |
| المساهمون: | Hôpital Central de Yaoundé Yaoundé, Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes (TransVIHMI), Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I (UY1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Université Cheikh Anta Diop de Dakar Sénégal (UCAD), Université de Yaoundé I (UY1), Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier) |
| المصدر: | ISSN: 1156-5233. |
| بيانات النشر: | HAL CCSD Elsevier Masson |
| سنة النشر: | 2015 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | Cryptococcose, Cryptococcosis, Diagnosis, Diagnostic, Fluconazole, Frequency, Fréquence, HIV infection, VIH, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology |
| الوصف: | International audience ; Cryptococcal meningitis is a mycosis encountered especially in patients with Acquired Immunodeficiency Syndrome and is fatal in the absence of treatment. Information on epidemiology, diagnosis and susceptibility profile to antifungal drugs, are scarce in Cameroon. Authors evaluated the diagnosis possibilities of the cryptococcal meningitis in Cameroon, and studied the antifungal susceptibility of isolated strains to fluconazole, used as first line treatment of the disease in Cameroon. Between December 2009 and July 2011, 146 cerebrospinal fluids obtained from HIV patients with suspicion of meningitis were analysed. The diagnosis procedure involved macroscopic and cyto-chemical analysis, India ink test, culture on Sabouraud chloramphenicol medium and antigen latex agglutination test. Antifungal susceptibility testing of isolated strains to fluconazole was done by the E-test(®) method. The diagnosis of cryptococcal meningitis gave 28.08% positive cases. Among these patients, 80% were at stages III and IV and 20% at stage I of the HIV infection, according to the WHO previous classification. Cyto-chemical analysis showed current findings in the case of cryptococcal meningitis. India ink test and latex agglutination test exhibited very high sensitivity and specificity (>94%). Fluconazole antifungal susceptibility testing gave MICs lower than 32μg/mL to 92.7% of isolated strains and MICs greater than this value to 7.3% of isolates. These results showed that cryptococcal meningitis remains a real problem among HIV infected patients in Yaoundé. The emergence of fluconazole reduced susceptibility strains is worrying. Nevertheless, efficacy of rapid detection tests is interesting because this will help in rapid diagnosis and treatment of patients. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/25467817; hal-02157606; https://hal.science/hal-02157606Test; PUBMED: 25467817 |
| DOI: | 10.1016/j.mycmed.2014.10.016 |
| الإتاحة: | https://doi.org/10.1016/j.mycmed.2014.10.016Test https://hal.science/hal-02157606Test |
| رقم الانضمام: | edsbas.C386E2A2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.mycmed.2014.10.016 |
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