Modular injectable matrices based on alginate solution/microsphere mixtures that gel in situ and co-deliver immunomodulatory factors
| العنوان: | Modular injectable matrices based on alginate solution/microsphere mixtures that gel in situ and co-deliver immunomodulatory factors |
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| المؤلفون: | Amy M. Winans, Darrell J. Irvine, Yuki Hori |
| المساهمون: | Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Materials Science and Engineering, Koch Institute for Integrative Cancer Research at MIT, Hori, Yuki, Winans, Amy M., Irvine, Darrell J. |
| المصدر: | PMC |
| بيانات النشر: | Elsevier BV, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | In situ, Sustained delivery, Materials science, Alginates, Biomedical Engineering, chemistry.chemical_element, Calcium, Biochemistry, Article, Injections, Microsphere, Biomaterials, Mice, chemistry.chemical_compound, Glucuronic Acid, Animals, Immunologic Factors, Molecular Biology, Hexuronic Acids, General Medicine, Cpg oligonucleotides, Glucuronic acid, Controlled release, Microspheres, Mice, Inbred C57BL, Solutions, chemistry, Chemical engineering, Self-healing hydrogels, Interleukin-2, CpG Islands, Gels, Biotechnology, Biomedical engineering |
| الوصف: | Biocompatible polymer solutions that can crosslink in situ following injection to form stable hydrogels are of interest as depots for sustained delivery of therapeutic factors or cells, and as scaffolds for regenerative medicine. Here, injectable self-gelling alginate formulations obtained by mixing alginate microspheres (as calcium reservoirs) with soluble alginate solutions were characterized for potential use in immunotherapy. Rapid redistribution of calcium ions from microspheres into the surrounding alginate solution led to crosslinking and formation of stable hydrogels. The mechanical properties of the resulting gels correlated with the concentration of calcium-reservoir microspheres added to the solution. Soluble factors such as the cytokine interleukin-2 were readily incorporated into self-gelling alginate matrices by simply mixing them with the formulation prior to gelation. Using alginate microspheres as modular components, strategies for binding immunostimulatory CpG oligonucleotides onto the surface of microspheres were also demonstrated. When injected subcutaneously in the flanks of mice, self-gelling alginate formed soft macroporous gels supporting cellular infiltration and allowing ready access to microspheres carrying therapeutic factors embedded in the matrix. This in situ gelling formulation may thus be useful for stimulating immune cells at desired locales, such as solid tumors or infection sites, as well as for other soft tissue regeneration applications. United States. Defense Advanced Research Projects Agency (Contract W81XWH-04-C-0139) National Institutes of Health (U.S.) (EB007280-02) National Science Foundation (U.S.) (Award 0348259) |
| وصف الملف: | application/pdf |
| تدمد: | 1742-7061 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::838134a0f52b23bbb042123812f0e6e1Test https://doi.org/10.1016/j.actbio.2008.11.019Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....838134a0f52b23bbb042123812f0e6e1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17427061 |
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