Rapid screening of methicillin-resistant Staphylococcus aureus using PCR and chromogenic agar: a prospective study to evaluate costs and effects
| العنوان: | Rapid screening of methicillin-resistant Staphylococcus aureus using PCR and chromogenic agar: a prospective study to evaluate costs and effects |
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| المؤلفون: | Steven F. T. Thijsen, Willem J. G. Melchers, Andreas Voss, Jan Kluytmans, A.G.M. Buiting, Marjan W. M. Wassenberg, G.A. de Wit, A. A. Van Zwet, Petra F. G. Wolffs, Christina M. J. E. Vandenbroucke-Grauls, A.T.A. Box, Annet Troelstra, M.W.H. Wulf, R.W. Bosboom, Caroline E. Visser, E.P.M. van Elzakker, Marc J. M. Bonten, M. M. L. Van Rijen |
| المساهمون: | Medische Microbiologie, RS: CAPHRI School for Public Health and Primary Care, Medical Microbiology and Infection Prevention, CCA - Innovative therapy, Amsterdam institute for Infection and Immunity |
| المصدر: | Clinical Microbiology and Infection, 16(12), 1754-1761. ELSEVIER SCI LTD Clinical Microbiology and Infection, 16, 12, pp. 1754-61 Clinical Microbiology and Infection, 16(12), 1754-1761 Wassenberg, M W M, Kluijtmans, J A J W, Box, A T A, Bosboom, R W, Buiting, A G M, van Elzakker, E P M, Melchers, W J G, van Rijen, M M L, Thijsen, S F T, Troelstra, A, Vandenbroucke-Grauls, C M J E, Visser, C E, Voss, A, Wolffs, P F G, Wulf, M W H, van Zwet, A A, de Wit, G A & Bonten, M J M 2010, ' Rapid screening of methicillin-resistant Staphylococcus aureus using PCR and chromogenic agar: a prospective study to evaluate costs and effects ', Clinical Microbiology and Infection, vol. 16, no. 12, pp. 1754-1761 . https://doi.org/10.1111/j.1469-0691.2010.03210.xTest Clinical microbiology and infection, 16(12), 1754-1761. Elsevier Limited Clinical Microbiology and Infection, 16, 1754-61 |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Methicillin-Resistant Staphylococcus aureus, Microbiology (medical), medicine.medical_specialty, food.ingredient, Micrococcaceae, Isolation (health care), Cost-Benefit Analysis, MRSA, medicine.disease_cause, Polymerase Chain Reaction, Microbiology, Patient Isolation, food, Predictive Value of Tests, cost analysis, Internal medicine, medicine, Humans, Agar, Chromogenic agar, Prospective Studies, Cross Infection, GeneXpert MTB/RIF, biology, Diagnostic Tests, Routine, Chromogenic, business.industry, Health Care Costs, General Medicine, Staphylococcal Infections, MRSA PCR, biology.organism_classification, Chromogenic Compounds, Methicillin-resistant Staphylococcus aureus, Pathogenesis and modulation of inflammation [N4i 1], Infectious Diseases, Staphylococcus aureus, Carrier State, business, rapid screening |
| الوصف: | Contains fulltext : 89508.pdf (Publisher’s version ) (Closed access) Pre-emptive isolation of suspected methicillin-resistant Staphylococcus aureus (MRSA) carriers is considered essential for controlling the spread of MRSA, but noncolonized patients will be isolated unnecessarily as a result of a delay in diagnosis of 3-5 days with conventional cultures. We determined costs per isolation day avoided, and incremental costs of rapid MRSA screening tests when added to conventional screening, but with decisions on isolation measures based on PCR results. A prospective multicentre study evaluating BD GeneOhm MRSA PCR (`IDI') (BD Diagnostics, San Diego, CA, USA), Xpert MRSA (`GeneXpert') (Cepheid, Sunnyvale, CA, USA) and chromogenic agar (MRSA-ID) (bioMerieux, Marcy-l'Etoile, France) was performed in 14 Dutch hospitals. Among 1764 patients at risk, MRSA prevalence was 3.3% (n=59). Duration of isolation was 19.7 and 16.1 h with IDI and GeneXpert, respectively, and would have been 30.0 and 76.2 h when based on chromogenic agar and conventional cultures, respectively. Negative predictive values (at a patient level) were 99.5%, 99.1% and 99.5% for IDI, GeneXpert and chromogenic agar, respectively. Numbers of isolation days were reduced by 60% and 47% with PCR-based and chromogenic agar-based screening, respectively. The cost per test was euro56.22 for IDI, euro69.62 for GeneXpert and euro2.08 for chromogenic agar, and additional costs per extra isolation day were euro26.34. Costs per isolation day avoided were euro95.77 (IDI) and euro125.43 (GeneXpert). PCR-based decision-making added euro153.64 (IDI) and euro193.84 (GeneXpert) per patient to overall costs and chromogenic testing would have saved euro30.79 per patient. Rapid diagnostic testing safely reduces the number of unnecessary isolation days, but only chromogenic screening, and not PCR-based screening, can be considered as cost saving. 01 december 2010 |
| وصف الملف: | application/pdf |
| تدمد: | 1198-743X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9937c0845d4e470a495331ef95fe5a26Test https://doi.org/10.1111/j.1469-0691.2010.03210.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9937c0845d4e470a495331ef95fe5a26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1198743X |
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