دورية أكاديمية
Temperature Sensitivity: A Potential Method for the Generation of Vaccines against the Avian Coronavirus Infectious Bronchitis Virus.
| العنوان: | Temperature Sensitivity: A Potential Method for the Generation of Vaccines against the Avian Coronavirus Infectious Bronchitis Virus. |
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| المؤلفون: | Keep, Sarah, Stevenson-Leggett, Phoebe, Steyn, Angela, Oade, Michael S, Webb, Isobel, Stuart, Jamie, Vervelde, Lonneke, Britton, Paul, Maier, Helena J, Bickerton, Erica |
| بيانات النشر: | MDPI AG //dx.doi.org/10.3390/v12070754 Viruses |
| سنة النشر: | 2020 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | IBV, RNA synthesis, coronavirus, replicase, temperature sensitivity, Animals, Cell Line, Chick Embryo, Chickens, Coronavirus Infections, Infectious bronchitis virus, Poultry, Poultry Diseases, RNA, Viral, Temperature, Vaccination, Vaccines, Attenuated, Viral Vaccines, Virus Replication |
| الوصف: | The Gammacoronavirus infectious bronchitis virus (IBV) is a highly contagious economically important respiratory pathogen of domestic fowl. Reverse genetics allows for the molecular study of pathogenic determinants to enable rational vaccine design. The recombinant IBV (rIBV) Beau-R, a molecular clone of the apathogenic Beaudette strain, has previously been investigated as a vaccine platform. To determine tissues in which Beau-R could effectively deliver antigenic genes, an in vivo study in chickens, the natural host, was used to compare the pattern of viral dissemination of Beau-R to the pathogenic strain M41-CK. Replication of Beau-R was found to be restricted to soft tissue within the beak, whereas M41-CK was detected in beak tissue, trachea and eyelid up to seven days post infection. In vitro assays further identified that, unlike M41-CK, Beau-R could not replicate at 41 °C, the core body temperature of a chicken, but is able to replicate a 37 °C, a temperature relatable to the very upper respiratory tract. Using a panel of rIBVs with defined mutations in the structural and accessory genes, viral replication at permissive and non-permissive temperatures was investigated, identifying that the Beau-R replicase gene was a determinant of temperature sensitivity and that sub-genomic mRNA synthesis had been affected. The identification of temperature sensitive allelic lesions within the Beau-R replicase gene opens up the possibility of using this method of attenuation in other IBV strains for future vaccine development as well as a method to investigate the functions of the IBV replicase proteins. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/zip; text/xml; application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/308053Test |
| DOI: | 10.17863/CAM.55148 |
| الإتاحة: | https://doi.org/10.17863/CAM.55148Test https://www.repository.cam.ac.uk/handle/1810/308053Test |
| حقوق: | Attribution 4.0 International (CC BY 4.0) ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.FAF33479 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.55148 |
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