دورية أكاديمية
Interferon-beta induces a long-lasting antiviral state in human respiratory epithelial cells
| العنوان: | Interferon-beta induces a long-lasting antiviral state in human respiratory epithelial cells |
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| المؤلفون: | Gaajetaan, G.R., Geelen, T.H., Vernooy, J.H.J., Dentener, M.A., Reynaert, N.L., Rohde, G.G., Beuken, E. V., Grauls, G.E., Bruggeman, C.A.M.V.A., Stassen, F.R.M. |
| المصدر: | Gaajetaan , G R , Geelen , T H , Vernooy , J H J , Dentener , M A , Reynaert , N L , Rohde , G G , Beuken , E V , Grauls , G E , Bruggeman , C A M V A & Stassen , F R M 2013 , ' Interferon-beta induces a long-lasting antiviral state in human respiratory epithelial cells ' , Journal of Infection , vol. 66 , no. 2 , pp. 163-169 . https://doi.org/10.1016/j.jinf.2012.11.008Test |
| سنة النشر: | 2013 |
| المجموعة: | Maastricht University Research Publications |
| مصطلحات موضوعية: | Interferon beta, Rhinovirus, A549 cells, Primary bronchial epithelial cells, Asthma, COPD, OBSTRUCTIVE PULMONARY-DISEASE, INTRANASAL INTERFERON-ALPHA-2, RHINOVIRUS INFECTIONS, ASTHMA EXACERBATIONS, VIRAL-INFECTIONS, VIRUSES, COLDS, ALPHA |
| الوصف: | OBJECTIVES: Interferon-beta (IFNbeta) induces strong antiviral effects and is therefore an attractive agent to prevent or reduce the incidence of virus-mediated exacerbations in asthmatic or chronic obstructive pulmonary disease (COPD) patients. We therefore investigated the effects of prophylactic IFNbeta on respiratory epithelial cells infected with rhinovirus (RV). METHODS: A549 cells and primary bronchial epithelial cells (PBECs) were exposed for 18 h to IFNbeta. Then, IFNbeta was either removed or maintained in the supernatant for the rest of the experiment and cells were infected with RV-1B at t = 0 or 72 h after the initial exposure to IFNbeta. RESULTS: Viral RNA levels were decreased in both cell types. Furthermore, both viral RNA and infectious virus levels in the supernatant of infected A549 cells were still significantly reduced at 72 h after removal of IFNbeta. This pronounced antiviral pre-treatment effect was associated with increased expression of the antiviral genes IFN-stimulated protein of MR15000 (ISG15) and Myxovirus resistance 1 (Mx1) and the effect was maintained even when IFNbeta levels in the supernatant of A549 cells were undetectable. CONCLUSIONS: These data show that IFNbeta has not only a strong, but also a long-lasting protective effect against RV infection of respiratory epithelium. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.jinf.2012.11.008 |
| الإتاحة: | https://doi.org/10.1016/j.jinf.2012.11.008Test https://cris.maastrichtuniversity.nl/en/publications/7b679d20-34ae-4059-876e-fa862cc0604dTest |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.5BAFF14A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jinf.2012.11.008 |
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