التفاصيل البيبلوغرافية
| العنوان: |
Calibration of two validated SARS-CoV-2 pseudovirus neutralization assays for COVID-19 vaccine evaluation. |
| المؤلفون: |
Huang, Yunda, Borisov, Oleg, Kee, Jia Jin, Carpp, Lindsay N., Wrin, Terri, Cai, Suqin, Sarzotti-Kelsoe, Marcella, McDanal, Charlene, Eaton, Amanda, Pajon, Rolando, Hural, John, Posavad, Christine M., Gill, Katherine, Karuna, Shelly, Corey, Lawrence, McElrath, M. Juliana, Gilbert, Peter B., Petropoulos, Christos J., Montefiori, David C. |
| المصدر: |
Scientific Reports; 12/14/2021, Vol. 11 Issue 1, p1-13, 13p |
| مصطلحات موضوعية: |
COVID-19 vaccines, SARS-CoV-2, CONVALESCENT plasma, COVID-19, VACCINE effectiveness, IMMUNOGLOBULINS |
| الشركة/الكيان: |
WORLD Health Organization |
| مستخلص: |
Vaccine-induced neutralizing antibodies (nAbs) are key biomarkers considered to be associated with vaccine efficacy. In United States government-sponsored phase 3 efficacy trials of COVID-19 vaccines, nAbs are measured by two different validated pseudovirus-based SARS-CoV-2 neutralization assays, with each trial using one of the two assays. Here we describe and compare the nAb titers obtained in the two assays. We observe that one assay consistently yielded higher nAb titers than the other when both assays were performed on the World Health Organization's anti-SARS-CoV-2 immunoglobulin International Standard, COVID-19 convalescent sera, and mRNA-1273 vaccinee sera. To overcome the challenge this difference in readout poses in comparing/combining data from the two assays, we evaluate three calibration approaches and show that readouts from the two assays can be calibrated to a common scale. These results may aid decision-making based on data from these assays for the evaluation and licensure of new or adapted COVID-19 vaccines. [ABSTRACT FROM AUTHOR] |
|
Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder