مؤتمر
Diversity of spoilage fungi in dairy products and environment, and their resistance to chemical preservatives
| العنوان: | Diversity of spoilage fungi in dairy products and environment, and their resistance to chemical preservatives |
|---|---|
| المؤلفون: | Garnier, L., Valence, Florence, Pawtowski, A., Galerne, L., Deniel, F., Frotté, N., Coton, E., Mounier, J. |
| المساهمون: | Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Université de Brest (UBO), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST |
| المصدر: | 3.International Conference on Microbial Diversity The challenge of Complexity https://hal.science/hal-01454545Test 3.International Conference on Microbial Diversity The challenge of Complexity, Oct 2015, Pérouse, Italy. , 2015 https://archives-publications.inrae.fr/331892.pdfTest |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2015 |
| المجموعة: | Université de Bretagne Occidentale: HAL |
| مصطلحات موضوعية: | contamination fongique, préservation chimique, diversité fongique, résistance chimique, food contamination, chemical treatment, food preservation, dairy product, resistance to chemicals, contamination alimentaire, traitement chimique, conservation des aliments, produit laitier, aliment santé pour l'homme, altération fongique, résistance aux produits chimiques, biodiversité fongique, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.IDA]Life Sciences [q-bio]/Food engineering |
| جغرافية الموضوع: | Pérouse, Italy |
| الوصف: | Fungal spoilage is one of the causes of consequential losses in the dairy industry. In this context, the use of bioprotective cultures can be an alternative or a complementary approach to be considered. Lactic acid bacteria (LAB) and propionibacteria, as well as some fungal species, can exhibit antifungal activities with large differences in activity between strains. Therefore, it is necessary to develop high-throughput screening methods to test a large number of strains and find the most efficient ones. In the present study, we developed a miniaturized high-throughput screening technique to rapidly detect antifungal activities in a cheese-like model. This model, distributed in a 24-well plate, consisted of 5-fold concentrated whole milk ultrafiltration retentate (final fat concentration of 45%), rennet (0.03%) and inoculated with a mesophilic lactic commercial starter and a pH indicator. Each well of the plate could be considered as a miniature cheese of ~2 g. Potent antifungal isolates were cultured in two dairy media; (i) a 10%-reconstituted low heat skim milk supplemented with 45% anhydrous milk fat (LH) and (ii) a 6-fold concentrated milk ultrafiltration permeate sterilized by 0.22 μm filtration and complemented with 10 g/l yeast extract and a pH indicator (UF). After cultivation, cultures (100 µl) were deposited on the miniature cheese surfaces followed by inoculation in duplicate with 50 spores or cells of 4 different fungal targets (1 fungi/plate), e.g., Mucor racemosus, Galactomyces geotrichum, Penicillium commune and Yarrowia lipolytica, and incubation at 12°C for up to 15 days. We screened 505 bacterial isolates belonging to Lactobacillus, Lactococcus, Pediococcus, Leuconostoc and Propiobacterium genera and 198 fungal isolates belonging to 28 genera. This high-throughput screening for antifungal activity revealed that 52 and 216 bacteria, and, 53 and 89 fungi, inhibited at least one fungal target after cultivation in UF and LH, respectively. Among the 4 tested fungal targets, P. commune was the most ... |
| نوع الوثيقة: | conference object still image |
| اللغة: | English |
| العلاقة: | hal-01454545; https://hal.science/hal-01454545Test; PRODINRA: 331892 |
| الإتاحة: | https://hal.science/hal-01454545Test |
| رقم الانضمام: | edsbas.B5A82186 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |